Job ID = 6459468 SRX = SRX681827 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:16:01 prefetch.2.10.7: 1) Downloading 'SRR1552296'... 2020-06-21T13:16:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:23:17 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:23:17 prefetch.2.10.7: 1) 'SRR1552296' was downloaded successfully Read 40755850 spots for SRR1552296/SRR1552296.sra Written 40755850 spots for SRR1552296/SRR1552296.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:43 40755850 reads; of these: 40755850 (100.00%) were unpaired; of these: 2820812 (6.92%) aligned 0 times 33531125 (82.27%) aligned exactly 1 time 4403913 (10.81%) aligned >1 times 93.08% overall alignment rate Time searching: 00:09:43 Overall time: 00:09:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6167183 / 37935038 = 0.1626 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:45:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:45:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:45:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:45:15: 1000000 INFO @ Sun, 21 Jun 2020 22:45:21: 2000000 INFO @ Sun, 21 Jun 2020 22:45:27: 3000000 INFO @ Sun, 21 Jun 2020 22:45:32: 4000000 INFO @ Sun, 21 Jun 2020 22:45:38: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:45:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:45:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:45:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:45:43: 6000000 INFO @ Sun, 21 Jun 2020 22:45:46: 1000000 INFO @ Sun, 21 Jun 2020 22:45:49: 7000000 INFO @ Sun, 21 Jun 2020 22:45:52: 2000000 INFO @ Sun, 21 Jun 2020 22:45:55: 8000000 INFO @ Sun, 21 Jun 2020 22:45:58: 3000000 INFO @ Sun, 21 Jun 2020 22:46:01: 9000000 INFO @ Sun, 21 Jun 2020 22:46:04: 4000000 INFO @ Sun, 21 Jun 2020 22:46:07: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:46:10: 5000000 INFO @ Sun, 21 Jun 2020 22:46:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:46:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:46:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:46:13: 11000000 INFO @ Sun, 21 Jun 2020 22:46:16: 6000000 INFO @ Sun, 21 Jun 2020 22:46:17: 1000000 INFO @ Sun, 21 Jun 2020 22:46:19: 12000000 INFO @ Sun, 21 Jun 2020 22:46:22: 7000000 INFO @ Sun, 21 Jun 2020 22:46:23: 2000000 INFO @ Sun, 21 Jun 2020 22:46:25: 13000000 INFO @ Sun, 21 Jun 2020 22:46:28: 8000000 INFO @ Sun, 21 Jun 2020 22:46:29: 3000000 INFO @ Sun, 21 Jun 2020 22:46:31: 14000000 INFO @ Sun, 21 Jun 2020 22:46:34: 9000000 INFO @ Sun, 21 Jun 2020 22:46:36: 4000000 INFO @ Sun, 21 Jun 2020 22:46:37: 15000000 INFO @ Sun, 21 Jun 2020 22:46:40: 10000000 INFO @ Sun, 21 Jun 2020 22:46:42: 5000000 INFO @ Sun, 21 Jun 2020 22:46:44: 16000000 INFO @ Sun, 21 Jun 2020 22:46:47: 11000000 INFO @ Sun, 21 Jun 2020 22:46:48: 6000000 INFO @ Sun, 21 Jun 2020 22:46:50: 17000000 INFO @ Sun, 21 Jun 2020 22:46:53: 12000000 INFO @ Sun, 21 Jun 2020 22:46:55: 7000000 INFO @ Sun, 21 Jun 2020 22:46:56: 18000000 INFO @ Sun, 21 Jun 2020 22:46:59: 13000000 INFO @ Sun, 21 Jun 2020 22:47:01: 8000000 INFO @ Sun, 21 Jun 2020 22:47:02: 19000000 INFO @ Sun, 21 Jun 2020 22:47:06: 14000000 INFO @ Sun, 21 Jun 2020 22:47:07: 9000000 INFO @ Sun, 21 Jun 2020 22:47:08: 20000000 INFO @ Sun, 21 Jun 2020 22:47:12: 15000000 INFO @ Sun, 21 Jun 2020 22:47:14: 10000000 INFO @ Sun, 21 Jun 2020 22:47:14: 21000000 INFO @ Sun, 21 Jun 2020 22:47:19: 16000000 INFO @ Sun, 21 Jun 2020 22:47:20: 22000000 INFO @ Sun, 21 Jun 2020 22:47:20: 11000000 INFO @ Sun, 21 Jun 2020 22:47:25: 17000000 INFO @ Sun, 21 Jun 2020 22:47:26: 23000000 INFO @ Sun, 21 Jun 2020 22:47:27: 12000000 INFO @ Sun, 21 Jun 2020 22:47:32: 18000000 INFO @ Sun, 21 Jun 2020 22:47:33: 24000000 INFO @ Sun, 21 Jun 2020 22:47:33: 13000000 INFO @ Sun, 21 Jun 2020 22:47:38: 19000000 INFO @ Sun, 21 Jun 2020 22:47:39: 25000000 INFO @ Sun, 21 Jun 2020 22:47:40: 14000000 INFO @ Sun, 21 Jun 2020 22:47:45: 20000000 INFO @ Sun, 21 Jun 2020 22:47:45: 26000000 INFO @ Sun, 21 Jun 2020 22:47:46: 15000000 INFO @ Sun, 21 Jun 2020 22:47:52: 21000000 INFO @ Sun, 21 Jun 2020 22:47:52: 27000000 INFO @ Sun, 21 Jun 2020 22:47:53: 16000000 INFO @ Sun, 21 Jun 2020 22:47:58: 22000000 INFO @ Sun, 21 Jun 2020 22:47:58: 28000000 INFO @ Sun, 21 Jun 2020 22:47:59: 17000000 INFO @ Sun, 21 Jun 2020 22:48:05: 29000000 INFO @ Sun, 21 Jun 2020 22:48:05: 23000000 INFO @ Sun, 21 Jun 2020 22:48:06: 18000000 INFO @ Sun, 21 Jun 2020 22:48:11: 30000000 INFO @ Sun, 21 Jun 2020 22:48:11: 24000000 INFO @ Sun, 21 Jun 2020 22:48:12: 19000000 INFO @ Sun, 21 Jun 2020 22:48:17: 31000000 INFO @ Sun, 21 Jun 2020 22:48:18: 25000000 INFO @ Sun, 21 Jun 2020 22:48:19: 20000000 INFO @ Sun, 21 Jun 2020 22:48:23: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:48:23: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:48:23: #1 total tags in treatment: 31767855 INFO @ Sun, 21 Jun 2020 22:48:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:48:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:48:24: #1 tags after filtering in treatment: 31767854 INFO @ Sun, 21 Jun 2020 22:48:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:48:24: #1 finished! INFO @ Sun, 21 Jun 2020 22:48:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:48:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:48:24: 26000000 INFO @ Sun, 21 Jun 2020 22:48:25: 21000000 INFO @ Sun, 21 Jun 2020 22:48:26: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 22:48:26: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:48:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:48:31: 22000000 INFO @ Sun, 21 Jun 2020 22:48:31: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:48:37: 23000000 INFO @ Sun, 21 Jun 2020 22:48:38: 28000000 INFO @ Sun, 21 Jun 2020 22:48:43: 24000000 INFO @ Sun, 21 Jun 2020 22:48:45: 29000000 INFO @ Sun, 21 Jun 2020 22:48:50: 25000000 INFO @ Sun, 21 Jun 2020 22:48:51: 30000000 INFO @ Sun, 21 Jun 2020 22:48:56: 26000000 INFO @ Sun, 21 Jun 2020 22:48:58: 31000000 INFO @ Sun, 21 Jun 2020 22:49:02: 27000000 INFO @ Sun, 21 Jun 2020 22:49:04: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:49:04: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:49:04: #1 total tags in treatment: 31767855 INFO @ Sun, 21 Jun 2020 22:49:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:49:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:49:05: #1 tags after filtering in treatment: 31767854 INFO @ Sun, 21 Jun 2020 22:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:49:05: #1 finished! INFO @ Sun, 21 Jun 2020 22:49:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:49:07: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 22:49:07: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:49:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:49:08: 28000000 INFO @ Sun, 21 Jun 2020 22:49:13: 29000000 INFO @ Sun, 21 Jun 2020 22:49:19: 30000000 INFO @ Sun, 21 Jun 2020 22:49:25: 31000000 INFO @ Sun, 21 Jun 2020 22:49:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:49:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:49:29: #1 total tags in treatment: 31767855 INFO @ Sun, 21 Jun 2020 22:49:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:49:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:49:30: #1 tags after filtering in treatment: 31767854 INFO @ Sun, 21 Jun 2020 22:49:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:49:30: #1 finished! INFO @ Sun, 21 Jun 2020 22:49:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:49:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:49:32: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 22:49:32: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:49:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX681827/SRX681827.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。