Job ID = 6626689 SRX = SRX6813093 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:57 14233466 reads; of these: 14233466 (100.00%) were unpaired; of these: 494156 (3.47%) aligned 0 times 11324132 (79.56%) aligned exactly 1 time 2415178 (16.97%) aligned >1 times 96.53% overall alignment rate Time searching: 00:02:57 Overall time: 00:02:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1387994 / 13739310 = 0.1010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:31: 1000000 INFO @ Tue, 14 Jul 2020 08:25:35: 2000000 INFO @ Tue, 14 Jul 2020 08:25:43: 3000000 INFO @ Tue, 14 Jul 2020 08:25:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:57: 5000000 INFO @ Tue, 14 Jul 2020 08:25:59: 1000000 INFO @ Tue, 14 Jul 2020 08:26:04: 2000000 INFO @ Tue, 14 Jul 2020 08:26:04: 6000000 INFO @ Tue, 14 Jul 2020 08:26:08: 3000000 INFO @ Tue, 14 Jul 2020 08:26:10: 7000000 INFO @ Tue, 14 Jul 2020 08:26:13: 4000000 INFO @ Tue, 14 Jul 2020 08:26:17: 8000000 INFO @ Tue, 14 Jul 2020 08:26:18: 5000000 BedGraph に変換中... INFO @ Tue, 14 Jul 2020 08:26:23: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:26:24: 9000000 INFO @ Tue, 14 Jul 2020 08:26:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:26:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:26:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:26:27: 7000000 INFO @ Tue, 14 Jul 2020 08:26:29: 1000000 INFO @ Tue, 14 Jul 2020 08:26:31: 10000000 INFO @ Tue, 14 Jul 2020 08:26:32: 8000000 INFO @ Tue, 14 Jul 2020 08:26:34: 2000000 INFO @ Tue, 14 Jul 2020 08:26:37: 9000000 INFO @ Tue, 14 Jul 2020 08:26:38: 11000000 INFO @ Tue, 14 Jul 2020 08:26:39: 3000000 INFO @ Tue, 14 Jul 2020 08:26:41: 10000000 INFO @ Tue, 14 Jul 2020 08:26:43: 4000000 INFO @ Tue, 14 Jul 2020 08:26:46: 12000000 INFO @ Tue, 14 Jul 2020 08:26:46: 11000000 INFO @ Tue, 14 Jul 2020 08:26:48: 5000000 INFO @ Tue, 14 Jul 2020 08:26:48: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:26:48: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:26:48: #1 total tags in treatment: 12351316 INFO @ Tue, 14 Jul 2020 08:26:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:49: #1 tags after filtering in treatment: 12351279 INFO @ Tue, 14 Jul 2020 08:26:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:26:49: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:50: #2 number of paired peaks: 3104 INFO @ Tue, 14 Jul 2020 08:26:50: start model_add_line... INFO @ Tue, 14 Jul 2020 08:26:50: start X-correlation... INFO @ Tue, 14 Jul 2020 08:26:50: end of X-cor INFO @ Tue, 14 Jul 2020 08:26:50: #2 finished! INFO @ Tue, 14 Jul 2020 08:26:50: #2 predicted fragment length is 185 bps INFO @ Tue, 14 Jul 2020 08:26:50: #2 alternative fragment length(s) may be 185 bps INFO @ Tue, 14 Jul 2020 08:26:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05_model.r INFO @ Tue, 14 Jul 2020 08:26:50: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:26:50: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:26:51: 12000000 INFO @ Tue, 14 Jul 2020 08:26:53: 6000000 INFO @ Tue, 14 Jul 2020 08:26:54: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:26:54: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:26:54: #1 total tags in treatment: 12351316 INFO @ Tue, 14 Jul 2020 08:26:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:54: #1 tags after filtering in treatment: 12351279 INFO @ Tue, 14 Jul 2020 08:26:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:26:54: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:55: #2 number of paired peaks: 3104 INFO @ Tue, 14 Jul 2020 08:26:55: start model_add_line... INFO @ Tue, 14 Jul 2020 08:26:55: start X-correlation... INFO @ Tue, 14 Jul 2020 08:26:55: end of X-cor INFO @ Tue, 14 Jul 2020 08:26:55: #2 finished! INFO @ Tue, 14 Jul 2020 08:26:55: #2 predicted fragment length is 185 bps INFO @ Tue, 14 Jul 2020 08:26:55: #2 alternative fragment length(s) may be 185 bps INFO @ Tue, 14 Jul 2020 08:26:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10_model.r INFO @ Tue, 14 Jul 2020 08:26:55: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:26:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:26:58: 7000000 INFO @ Tue, 14 Jul 2020 08:27:03: 8000000 INFO @ Tue, 14 Jul 2020 08:27:09: 9000000 INFO @ Tue, 14 Jul 2020 08:27:14: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:27:20: 11000000 INFO @ Tue, 14 Jul 2020 08:27:21: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:27:25: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:27:25: 12000000 INFO @ Tue, 14 Jul 2020 08:27:28: #1 tag size is determined as 51 bps INFO @ Tue, 14 Jul 2020 08:27:28: #1 tag size = 51 INFO @ Tue, 14 Jul 2020 08:27:28: #1 total tags in treatment: 12351316 INFO @ Tue, 14 Jul 2020 08:27:28: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:27:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:27:28: #1 tags after filtering in treatment: 12351279 INFO @ Tue, 14 Jul 2020 08:27:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:27:28: #1 finished! INFO @ Tue, 14 Jul 2020 08:27:28: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:27:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:27:29: #2 number of paired peaks: 3104 INFO @ Tue, 14 Jul 2020 08:27:29: start model_add_line... INFO @ Tue, 14 Jul 2020 08:27:29: start X-correlation... INFO @ Tue, 14 Jul 2020 08:27:29: end of X-cor INFO @ Tue, 14 Jul 2020 08:27:29: #2 finished! INFO @ Tue, 14 Jul 2020 08:27:29: #2 predicted fragment length is 185 bps INFO @ Tue, 14 Jul 2020 08:27:29: #2 alternative fragment length(s) may be 185 bps INFO @ Tue, 14 Jul 2020 08:27:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20_model.r INFO @ Tue, 14 Jul 2020 08:27:29: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:27:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:27:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05_peaks.xls INFO @ Tue, 14 Jul 2020 08:27:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:27:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.05_summits.bed INFO @ Tue, 14 Jul 2020 08:27:36: Done! pass1 - making usageList (309 chroms): 2 millis pass2 - checking and writing primary data (4161 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:27:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10_peaks.xls INFO @ Tue, 14 Jul 2020 08:27:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:27:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.10_summits.bed INFO @ Tue, 14 Jul 2020 08:27:38: Done! pass1 - making usageList (212 chroms): 1 millis pass2 - checking and writing primary data (1482 records, 4 fields): 8 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:27:59: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 08:28:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20_peaks.xls INFO @ Tue, 14 Jul 2020 08:28:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 08:28:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX6813093/SRX6813093.20_summits.bed INFO @ Tue, 14 Jul 2020 08:28:12: Done! pass1 - making usageList (113 chroms): 1 millis pass2 - checking and writing primary data (418 records, 4 fields): 4 millis CompletedMACS2peakCalling