Job ID = 6459251 SRX = SRX6468494 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:00:34 prefetch.2.10.7: 1) Downloading 'SRR9710580'... 2020-06-21T13:00:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:05:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:05:44 prefetch.2.10.7: 1) 'SRR9710580' was downloaded successfully Read 30070766 spots for SRR9710580/SRR9710580.sra Written 30070766 spots for SRR9710580/SRR9710580.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:25 30070766 reads; of these: 30070766 (100.00%) were unpaired; of these: 1514545 (5.04%) aligned 0 times 19618257 (65.24%) aligned exactly 1 time 8937964 (29.72%) aligned >1 times 94.96% overall alignment rate Time searching: 00:08:25 Overall time: 00:08:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8892109 / 28556221 = 0.3114 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:22:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:22:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:22:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:22:14: 1000000 INFO @ Sun, 21 Jun 2020 22:22:19: 2000000 INFO @ Sun, 21 Jun 2020 22:22:24: 3000000 INFO @ Sun, 21 Jun 2020 22:22:29: 4000000 INFO @ Sun, 21 Jun 2020 22:22:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:22:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:22:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:22:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:22:39: 6000000 INFO @ Sun, 21 Jun 2020 22:22:44: 1000000 INFO @ Sun, 21 Jun 2020 22:22:44: 7000000 INFO @ Sun, 21 Jun 2020 22:22:50: 2000000 INFO @ Sun, 21 Jun 2020 22:22:50: 8000000 INFO @ Sun, 21 Jun 2020 22:22:55: 3000000 INFO @ Sun, 21 Jun 2020 22:22:55: 9000000 INFO @ Sun, 21 Jun 2020 22:23:00: 4000000 INFO @ Sun, 21 Jun 2020 22:23:00: 10000000 INFO @ Sun, 21 Jun 2020 22:23:06: 5000000 INFO @ Sun, 21 Jun 2020 22:23:06: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:23:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:23:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:23:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:23:11: 6000000 INFO @ Sun, 21 Jun 2020 22:23:11: 12000000 INFO @ Sun, 21 Jun 2020 22:23:15: 1000000 INFO @ Sun, 21 Jun 2020 22:23:17: 7000000 INFO @ Sun, 21 Jun 2020 22:23:17: 13000000 INFO @ Sun, 21 Jun 2020 22:23:21: 2000000 INFO @ Sun, 21 Jun 2020 22:23:22: 8000000 INFO @ Sun, 21 Jun 2020 22:23:22: 14000000 INFO @ Sun, 21 Jun 2020 22:23:26: 3000000 INFO @ Sun, 21 Jun 2020 22:23:28: 15000000 INFO @ Sun, 21 Jun 2020 22:23:28: 9000000 INFO @ Sun, 21 Jun 2020 22:23:32: 4000000 INFO @ Sun, 21 Jun 2020 22:23:33: 10000000 INFO @ Sun, 21 Jun 2020 22:23:34: 16000000 INFO @ Sun, 21 Jun 2020 22:23:37: 5000000 INFO @ Sun, 21 Jun 2020 22:23:39: 11000000 INFO @ Sun, 21 Jun 2020 22:23:39: 17000000 INFO @ Sun, 21 Jun 2020 22:23:43: 6000000 INFO @ Sun, 21 Jun 2020 22:23:44: 12000000 INFO @ Sun, 21 Jun 2020 22:23:45: 18000000 INFO @ Sun, 21 Jun 2020 22:23:48: 7000000 INFO @ Sun, 21 Jun 2020 22:23:50: 13000000 INFO @ Sun, 21 Jun 2020 22:23:50: 19000000 INFO @ Sun, 21 Jun 2020 22:23:54: 8000000 INFO @ Sun, 21 Jun 2020 22:23:54: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:23:54: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:23:54: #1 total tags in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:23:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:23:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:23:54: #1 tags after filtering in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:23:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:23:54: #1 finished! INFO @ Sun, 21 Jun 2020 22:23:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:23:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:23:55: 14000000 INFO @ Sun, 21 Jun 2020 22:23:56: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 22:23:56: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:23:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:23:59: 9000000 INFO @ Sun, 21 Jun 2020 22:24:01: 15000000 INFO @ Sun, 21 Jun 2020 22:24:05: 10000000 INFO @ Sun, 21 Jun 2020 22:24:07: 16000000 INFO @ Sun, 21 Jun 2020 22:24:10: 11000000 INFO @ Sun, 21 Jun 2020 22:24:12: 17000000 INFO @ Sun, 21 Jun 2020 22:24:16: 12000000 INFO @ Sun, 21 Jun 2020 22:24:18: 18000000 INFO @ Sun, 21 Jun 2020 22:24:21: 13000000 INFO @ Sun, 21 Jun 2020 22:24:23: 19000000 INFO @ Sun, 21 Jun 2020 22:24:27: 14000000 INFO @ Sun, 21 Jun 2020 22:24:27: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:24:27: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:24:27: #1 total tags in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:24:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:24:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:24:27: #1 tags after filtering in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:24:27: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:24:27: #1 finished! INFO @ Sun, 21 Jun 2020 22:24:27: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:24:27: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:24:29: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 22:24:29: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:24:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:24:32: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:24:37: 16000000 INFO @ Sun, 21 Jun 2020 22:24:43: 17000000 INFO @ Sun, 21 Jun 2020 22:24:48: 18000000 INFO @ Sun, 21 Jun 2020 22:24:53: 19000000 INFO @ Sun, 21 Jun 2020 22:24:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:24:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:24:57: #1 total tags in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:24:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:24:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:24:57: #1 tags after filtering in treatment: 19664112 INFO @ Sun, 21 Jun 2020 22:24:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:24:57: #1 finished! INFO @ Sun, 21 Jun 2020 22:24:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:24:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:24:58: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 22:24:58: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:24:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468494/SRX6468494.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。