Job ID = 6459248 SRX = SRX6468491 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:00:01 prefetch.2.10.7: 1) Downloading 'SRR9710577'... 2020-06-21T13:00:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:04:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:04:16 prefetch.2.10.7: 1) 'SRR9710577' was downloaded successfully Read 31565794 spots for SRR9710577/SRR9710577.sra Written 31565794 spots for SRR9710577/SRR9710577.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:58 31565794 reads; of these: 31565794 (100.00%) were unpaired; of these: 1774194 (5.62%) aligned 0 times 23761355 (75.28%) aligned exactly 1 time 6030245 (19.10%) aligned >1 times 94.38% overall alignment rate Time searching: 00:07:58 Overall time: 00:07:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15267040 / 29791600 = 0.5125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:10: 1000000 INFO @ Sun, 21 Jun 2020 22:20:15: 2000000 INFO @ Sun, 21 Jun 2020 22:20:20: 3000000 INFO @ Sun, 21 Jun 2020 22:20:25: 4000000 INFO @ Sun, 21 Jun 2020 22:20:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:35: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:35: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:35: 6000000 INFO @ Sun, 21 Jun 2020 22:20:40: 1000000 INFO @ Sun, 21 Jun 2020 22:20:41: 7000000 INFO @ Sun, 21 Jun 2020 22:20:45: 2000000 INFO @ Sun, 21 Jun 2020 22:20:46: 8000000 INFO @ Sun, 21 Jun 2020 22:20:50: 3000000 INFO @ Sun, 21 Jun 2020 22:20:51: 9000000 INFO @ Sun, 21 Jun 2020 22:20:56: 4000000 INFO @ Sun, 21 Jun 2020 22:20:56: 10000000 INFO @ Sun, 21 Jun 2020 22:21:01: 5000000 INFO @ Sun, 21 Jun 2020 22:21:02: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:21:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:21:05: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:21:05: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:21:07: 6000000 INFO @ Sun, 21 Jun 2020 22:21:08: 12000000 INFO @ Sun, 21 Jun 2020 22:21:10: 1000000 INFO @ Sun, 21 Jun 2020 22:21:12: 7000000 INFO @ Sun, 21 Jun 2020 22:21:13: 13000000 INFO @ Sun, 21 Jun 2020 22:21:16: 2000000 INFO @ Sun, 21 Jun 2020 22:21:18: 8000000 INFO @ Sun, 21 Jun 2020 22:21:19: 14000000 INFO @ Sun, 21 Jun 2020 22:21:21: 3000000 INFO @ Sun, 21 Jun 2020 22:21:22: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:21:22: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:21:22: #1 total tags in treatment: 14524560 INFO @ Sun, 21 Jun 2020 22:21:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:21:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:21:22: #1 tags after filtering in treatment: 14524545 INFO @ Sun, 21 Jun 2020 22:21:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:21:22: #1 finished! INFO @ Sun, 21 Jun 2020 22:21:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:21:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:21:23: 9000000 INFO @ Sun, 21 Jun 2020 22:21:23: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 22:21:23: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:21:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:21:27: 4000000 INFO @ Sun, 21 Jun 2020 22:21:29: 10000000 INFO @ Sun, 21 Jun 2020 22:21:32: 5000000 INFO @ Sun, 21 Jun 2020 22:21:34: 11000000 INFO @ Sun, 21 Jun 2020 22:21:38: 6000000 INFO @ Sun, 21 Jun 2020 22:21:40: 12000000 INFO @ Sun, 21 Jun 2020 22:21:43: 7000000 INFO @ Sun, 21 Jun 2020 22:21:45: 13000000 INFO @ Sun, 21 Jun 2020 22:21:49: 8000000 INFO @ Sun, 21 Jun 2020 22:21:51: 14000000 INFO @ Sun, 21 Jun 2020 22:21:54: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:21:54: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:21:54: #1 total tags in treatment: 14524560 INFO @ Sun, 21 Jun 2020 22:21:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:21:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:21:54: 9000000 INFO @ Sun, 21 Jun 2020 22:21:54: #1 tags after filtering in treatment: 14524545 INFO @ Sun, 21 Jun 2020 22:21:54: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:21:54: #1 finished! INFO @ Sun, 21 Jun 2020 22:21:54: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:21:54: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:21:55: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 22:21:55: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:21:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:21:59: 10000000 INFO @ Sun, 21 Jun 2020 22:22:04: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:22:10: 12000000 INFO @ Sun, 21 Jun 2020 22:22:15: 13000000 INFO @ Sun, 21 Jun 2020 22:22:20: 14000000 INFO @ Sun, 21 Jun 2020 22:22:23: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:22:23: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:22:23: #1 total tags in treatment: 14524560 INFO @ Sun, 21 Jun 2020 22:22:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:24: #1 tags after filtering in treatment: 14524545 INFO @ Sun, 21 Jun 2020 22:22:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:24: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:25: #2 number of paired peaks: 60 WARNING @ Sun, 21 Jun 2020 22:22:25: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468491/SRX6468491.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。