Job ID = 6459247 SRX = SRX6468490 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:01:34 prefetch.2.10.7: 1) Downloading 'SRR9710576'... 2020-06-21T13:01:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:05:59 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:05:59 prefetch.2.10.7: 1) 'SRR9710576' was downloaded successfully Read 28868123 spots for SRR9710576/SRR9710576.sra Written 28868123 spots for SRR9710576/SRR9710576.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:09 28868123 reads; of these: 28868123 (100.00%) were unpaired; of these: 1747681 (6.05%) aligned 0 times 20870744 (72.30%) aligned exactly 1 time 6249698 (21.65%) aligned >1 times 93.95% overall alignment rate Time searching: 00:07:09 Overall time: 00:07:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10697683 / 27120442 = 0.3945 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:33: 1000000 INFO @ Sun, 21 Jun 2020 22:20:39: 2000000 INFO @ Sun, 21 Jun 2020 22:20:45: 3000000 INFO @ Sun, 21 Jun 2020 22:20:52: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:58: 5000000 INFO @ Sun, 21 Jun 2020 22:21:03: 1000000 INFO @ Sun, 21 Jun 2020 22:21:04: 6000000 INFO @ Sun, 21 Jun 2020 22:21:10: 2000000 INFO @ Sun, 21 Jun 2020 22:21:11: 7000000 INFO @ Sun, 21 Jun 2020 22:21:17: 3000000 INFO @ Sun, 21 Jun 2020 22:21:18: 8000000 INFO @ Sun, 21 Jun 2020 22:21:23: 4000000 INFO @ Sun, 21 Jun 2020 22:21:24: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:21:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:21:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:21:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:21:30: 5000000 INFO @ Sun, 21 Jun 2020 22:21:31: 10000000 INFO @ Sun, 21 Jun 2020 22:21:33: 1000000 INFO @ Sun, 21 Jun 2020 22:21:36: 6000000 INFO @ Sun, 21 Jun 2020 22:21:37: 11000000 INFO @ Sun, 21 Jun 2020 22:21:40: 2000000 INFO @ Sun, 21 Jun 2020 22:21:43: 7000000 INFO @ Sun, 21 Jun 2020 22:21:44: 12000000 INFO @ Sun, 21 Jun 2020 22:21:47: 3000000 INFO @ Sun, 21 Jun 2020 22:21:49: 8000000 INFO @ Sun, 21 Jun 2020 22:21:50: 13000000 INFO @ Sun, 21 Jun 2020 22:21:53: 4000000 INFO @ Sun, 21 Jun 2020 22:21:56: 9000000 INFO @ Sun, 21 Jun 2020 22:21:57: 14000000 INFO @ Sun, 21 Jun 2020 22:22:00: 5000000 INFO @ Sun, 21 Jun 2020 22:22:03: 10000000 INFO @ Sun, 21 Jun 2020 22:22:04: 15000000 INFO @ Sun, 21 Jun 2020 22:22:07: 6000000 INFO @ Sun, 21 Jun 2020 22:22:10: 11000000 INFO @ Sun, 21 Jun 2020 22:22:10: 16000000 INFO @ Sun, 21 Jun 2020 22:22:13: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:22:13: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:22:13: #1 total tags in treatment: 16422759 INFO @ Sun, 21 Jun 2020 22:22:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:13: 7000000 INFO @ Sun, 21 Jun 2020 22:22:14: #1 tags after filtering in treatment: 16422753 INFO @ Sun, 21 Jun 2020 22:22:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:14: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:15: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 22:22:15: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:22:17: 12000000 INFO @ Sun, 21 Jun 2020 22:22:20: 8000000 INFO @ Sun, 21 Jun 2020 22:22:23: 13000000 INFO @ Sun, 21 Jun 2020 22:22:27: 9000000 INFO @ Sun, 21 Jun 2020 22:22:30: 14000000 INFO @ Sun, 21 Jun 2020 22:22:34: 10000000 INFO @ Sun, 21 Jun 2020 22:22:37: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:22:40: 11000000 INFO @ Sun, 21 Jun 2020 22:22:44: 16000000 INFO @ Sun, 21 Jun 2020 22:22:46: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:22:46: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:22:46: #1 total tags in treatment: 16422759 INFO @ Sun, 21 Jun 2020 22:22:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:47: 12000000 INFO @ Sun, 21 Jun 2020 22:22:47: #1 tags after filtering in treatment: 16422753 INFO @ Sun, 21 Jun 2020 22:22:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:47: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:48: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 22:22:48: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:22:53: 13000000 INFO @ Sun, 21 Jun 2020 22:23:00: 14000000 INFO @ Sun, 21 Jun 2020 22:23:06: 15000000 INFO @ Sun, 21 Jun 2020 22:23:13: 16000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:23:16: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 22:23:16: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 22:23:16: #1 total tags in treatment: 16422759 INFO @ Sun, 21 Jun 2020 22:23:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:23:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:23:16: #1 tags after filtering in treatment: 16422753 INFO @ Sun, 21 Jun 2020 22:23:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:23:16: #1 finished! INFO @ Sun, 21 Jun 2020 22:23:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:23:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:23:17: #2 number of paired peaks: 38 WARNING @ Sun, 21 Jun 2020 22:23:17: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:23:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468490/SRX6468490.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling