Job ID = 6459245 SRX = SRX6468488 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T13:13:04 prefetch.2.10.7: 1) Downloading 'SRR9710574'... 2020-06-21T13:13:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:20:20 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:20:20 prefetch.2.10.7: 1) 'SRR9710574' was downloaded successfully Read 31411937 spots for SRR9710574/SRR9710574.sra Written 31411937 spots for SRR9710574/SRR9710574.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:06 31411937 reads; of these: 31411937 (100.00%) were unpaired; of these: 1892108 (6.02%) aligned 0 times 22312477 (71.03%) aligned exactly 1 time 7207352 (22.94%) aligned >1 times 93.98% overall alignment rate Time searching: 00:08:06 Overall time: 00:08:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12204274 / 29519829 = 0.4134 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:36:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:36:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:36:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:36:15: 1000000 INFO @ Sun, 21 Jun 2020 22:36:20: 2000000 INFO @ Sun, 21 Jun 2020 22:36:26: 3000000 INFO @ Sun, 21 Jun 2020 22:36:31: 4000000 INFO @ Sun, 21 Jun 2020 22:36:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:36:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:36:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:36:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:36:42: 6000000 INFO @ Sun, 21 Jun 2020 22:36:45: 1000000 INFO @ Sun, 21 Jun 2020 22:36:48: 7000000 INFO @ Sun, 21 Jun 2020 22:36:51: 2000000 INFO @ Sun, 21 Jun 2020 22:36:54: 8000000 INFO @ Sun, 21 Jun 2020 22:36:57: 3000000 INFO @ Sun, 21 Jun 2020 22:37:00: 9000000 INFO @ Sun, 21 Jun 2020 22:37:03: 4000000 INFO @ Sun, 21 Jun 2020 22:37:06: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:37:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:37:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:37:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:37:09: 5000000 INFO @ Sun, 21 Jun 2020 22:37:12: 11000000 INFO @ Sun, 21 Jun 2020 22:37:15: 6000000 INFO @ Sun, 21 Jun 2020 22:37:16: 1000000 INFO @ Sun, 21 Jun 2020 22:37:18: 12000000 INFO @ Sun, 21 Jun 2020 22:37:22: 7000000 INFO @ Sun, 21 Jun 2020 22:37:22: 2000000 INFO @ Sun, 21 Jun 2020 22:37:24: 13000000 INFO @ Sun, 21 Jun 2020 22:37:28: 8000000 INFO @ Sun, 21 Jun 2020 22:37:28: 3000000 INFO @ Sun, 21 Jun 2020 22:37:31: 14000000 INFO @ Sun, 21 Jun 2020 22:37:34: 9000000 INFO @ Sun, 21 Jun 2020 22:37:34: 4000000 INFO @ Sun, 21 Jun 2020 22:37:37: 15000000 INFO @ Sun, 21 Jun 2020 22:37:40: 10000000 INFO @ Sun, 21 Jun 2020 22:37:41: 5000000 INFO @ Sun, 21 Jun 2020 22:37:43: 16000000 INFO @ Sun, 21 Jun 2020 22:37:46: 11000000 INFO @ Sun, 21 Jun 2020 22:37:47: 6000000 INFO @ Sun, 21 Jun 2020 22:37:49: 17000000 INFO @ Sun, 21 Jun 2020 22:37:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:37:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:37:51: #1 total tags in treatment: 17315555 INFO @ Sun, 21 Jun 2020 22:37:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:37:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:37:52: #1 tags after filtering in treatment: 17315553 INFO @ Sun, 21 Jun 2020 22:37:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:37:52: #1 finished! INFO @ Sun, 21 Jun 2020 22:37:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:37:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:37:52: 12000000 INFO @ Sun, 21 Jun 2020 22:37:53: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:37:53: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:37:53: 7000000 INFO @ Sun, 21 Jun 2020 22:37:58: 13000000 INFO @ Sun, 21 Jun 2020 22:37:59: 8000000 INFO @ Sun, 21 Jun 2020 22:38:05: 14000000 INFO @ Sun, 21 Jun 2020 22:38:05: 9000000 INFO @ Sun, 21 Jun 2020 22:38:11: 15000000 INFO @ Sun, 21 Jun 2020 22:38:12: 10000000 INFO @ Sun, 21 Jun 2020 22:38:17: 16000000 INFO @ Sun, 21 Jun 2020 22:38:18: 11000000 INFO @ Sun, 21 Jun 2020 22:38:23: 17000000 INFO @ Sun, 21 Jun 2020 22:38:24: 12000000 INFO @ Sun, 21 Jun 2020 22:38:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:38:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:38:25: #1 total tags in treatment: 17315555 INFO @ Sun, 21 Jun 2020 22:38:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:38:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:38:26: #1 tags after filtering in treatment: 17315553 INFO @ Sun, 21 Jun 2020 22:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:38:26: #1 finished! INFO @ Sun, 21 Jun 2020 22:38:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:38:27: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:38:27: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:38:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:38:30: 13000000 INFO @ Sun, 21 Jun 2020 22:38:36: 14000000 INFO @ Sun, 21 Jun 2020 22:38:41: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:38:47: 16000000 INFO @ Sun, 21 Jun 2020 22:38:53: 17000000 INFO @ Sun, 21 Jun 2020 22:38:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:38:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:38:55: #1 total tags in treatment: 17315555 INFO @ Sun, 21 Jun 2020 22:38:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:38:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:38:55: #1 tags after filtering in treatment: 17315553 INFO @ Sun, 21 Jun 2020 22:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:38:55: #1 finished! INFO @ Sun, 21 Jun 2020 22:38:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:38:56: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:38:56: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:38:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX6468488/SRX6468488.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。