Job ID = 6626614 SRX = SRX5734954 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:01 19743692 reads; of these: 19743692 (100.00%) were unpaired; of these: 3445647 (17.45%) aligned 0 times 14599948 (73.95%) aligned exactly 1 time 1698097 (8.60%) aligned >1 times 82.55% overall alignment rate Time searching: 00:06:01 Overall time: 00:06:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7189112 / 16298045 = 0.4411 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:29: 1000000 INFO @ Tue, 14 Jul 2020 08:04:34: 2000000 INFO @ Tue, 14 Jul 2020 08:04:40: 3000000 INFO @ Tue, 14 Jul 2020 08:04:46: 4000000 INFO @ Tue, 14 Jul 2020 08:04:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:57: 6000000 INFO @ Tue, 14 Jul 2020 08:05:00: 1000000 INFO @ Tue, 14 Jul 2020 08:05:03: 7000000 INFO @ Tue, 14 Jul 2020 08:05:07: 2000000 INFO @ Tue, 14 Jul 2020 08:05:09: 8000000 INFO @ Tue, 14 Jul 2020 08:05:14: 3000000 INFO @ Tue, 14 Jul 2020 08:05:15: 9000000 INFO @ Tue, 14 Jul 2020 08:05:16: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:05:16: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:05:16: #1 total tags in treatment: 9108933 INFO @ Tue, 14 Jul 2020 08:05:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:16: #1 tags after filtering in treatment: 9108403 INFO @ Tue, 14 Jul 2020 08:05:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:16: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:17: #2 number of paired peaks: 3782 INFO @ Tue, 14 Jul 2020 08:05:17: start model_add_line... INFO @ Tue, 14 Jul 2020 08:05:17: start X-correlation... INFO @ Tue, 14 Jul 2020 08:05:17: end of X-cor INFO @ Tue, 14 Jul 2020 08:05:17: #2 finished! INFO @ Tue, 14 Jul 2020 08:05:17: #2 predicted fragment length is 0 bps INFO @ Tue, 14 Jul 2020 08:05:17: #2 alternative fragment length(s) may be 0 bps INFO @ Tue, 14 Jul 2020 08:05:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.05_model.r WARNING @ Tue, 14 Jul 2020 08:05:17: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 08:05:17: #2 You may need to consider one of the other alternative d(s): 0 WARNING @ Tue, 14 Jul 2020 08:05:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 08:05:17: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:05:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:05:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:05:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:05:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:05:28: 5000000 INFO @ Tue, 14 Jul 2020 08:05:29: 1000000 INFO @ Tue, 14 Jul 2020 08:05:35: 6000000 INFO @ Tue, 14 Jul 2020 08:05:36: 2000000 INFO @ Tue, 14 Jul 2020 08:05:42: 3000000 INFO @ Tue, 14 Jul 2020 08:05:42: 7000000 INFO @ Tue, 14 Jul 2020 08:05:48: 4000000 INFO @ Tue, 14 Jul 2020 08:05:49: 8000000 INFO @ Tue, 14 Jul 2020 08:05:54: 5000000 INFO @ Tue, 14 Jul 2020 08:05:56: 9000000 INFO @ Tue, 14 Jul 2020 08:05:57: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:05:57: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:05:57: #1 total tags in treatment: 9108933 INFO @ Tue, 14 Jul 2020 08:05:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:57: #1 tags after filtering in treatment: 9108403 INFO @ Tue, 14 Jul 2020 08:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:57: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:58: #2 number of paired peaks: 3782 INFO @ Tue, 14 Jul 2020 08:05:58: start model_add_line... INFO @ Tue, 14 Jul 2020 08:05:58: start X-correlation... INFO @ Tue, 14 Jul 2020 08:05:58: end of X-cor INFO @ Tue, 14 Jul 2020 08:05:58: #2 finished! INFO @ Tue, 14 Jul 2020 08:05:58: #2 predicted fragment length is 0 bps INFO @ Tue, 14 Jul 2020 08:05:58: #2 alternative fragment length(s) may be 0 bps INFO @ Tue, 14 Jul 2020 08:05:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.10_model.r WARNING @ Tue, 14 Jul 2020 08:05:58: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 08:05:58: #2 You may need to consider one of the other alternative d(s): 0 WARNING @ Tue, 14 Jul 2020 08:05:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 08:05:58: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:05:58: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 08:06:00: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:06:06: 7000000 INFO @ Tue, 14 Jul 2020 08:06:12: 8000000 INFO @ Tue, 14 Jul 2020 08:06:18: 9000000 INFO @ Tue, 14 Jul 2020 08:06:18: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:06:18: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:06:18: #1 total tags in treatment: 9108933 INFO @ Tue, 14 Jul 2020 08:06:18: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:06:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:06:19: #1 tags after filtering in treatment: 9108403 INFO @ Tue, 14 Jul 2020 08:06:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:06:19: #1 finished! INFO @ Tue, 14 Jul 2020 08:06:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:06:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:06:20: #2 number of paired peaks: 3782 INFO @ Tue, 14 Jul 2020 08:06:20: start model_add_line... INFO @ Tue, 14 Jul 2020 08:06:20: start X-correlation... INFO @ Tue, 14 Jul 2020 08:06:20: end of X-cor INFO @ Tue, 14 Jul 2020 08:06:20: #2 finished! INFO @ Tue, 14 Jul 2020 08:06:20: #2 predicted fragment length is 0 bps INFO @ Tue, 14 Jul 2020 08:06:20: #2 alternative fragment length(s) may be 0 bps INFO @ Tue, 14 Jul 2020 08:06:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5734954/SRX5734954.20_model.r WARNING @ Tue, 14 Jul 2020 08:06:20: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 08:06:20: #2 You may need to consider one of the other alternative d(s): 0 WARNING @ Tue, 14 Jul 2020 08:06:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 08:06:20: #3 Call peaks... INFO @ Tue, 14 Jul 2020 08:06:20: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 /var/spool/uge/at154/job_scripts/6626614: line 297: 103343 Terminated MACS $i /var/spool/uge/at154/job_scripts/6626614: line 297: 103470 Terminated MACS $i /var/spool/uge/at154/job_scripts/6626614: line 297: 103608 Terminated MACS $i ls: cannot access SRX5734954.05.bed: No such file or directory mv: cannot stat ‘SRX5734954.05.bed’: No such file or directory mv: cannot stat ‘SRX5734954.05.bb’: No such file or directory ls: cannot access SRX5734954.10.bed: No such file or directory mv: cannot stat ‘SRX5734954.10.bed’: No such file or directory mv: cannot stat ‘SRX5734954.10.bb’: No such file or directory ls: cannot access SRX5734954.20.bed: No such file or directory mv: cannot stat ‘SRX5734954.20.bed’: No such file or directory mv: cannot stat ‘SRX5734954.20.bb’: No such file or directory