Job ID = 6626615 SRX = SRX5734952 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:52 21471874 reads; of these: 21471874 (100.00%) were unpaired; of these: 3995830 (18.61%) aligned 0 times 15633789 (72.81%) aligned exactly 1 time 1842255 (8.58%) aligned >1 times 81.39% overall alignment rate Time searching: 00:06:52 Overall time: 00:06:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7957251 / 17476044 = 0.4553 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:06:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:06:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:06:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:06:52: 1000000 INFO @ Tue, 14 Jul 2020 08:06:58: 2000000 INFO @ Tue, 14 Jul 2020 08:07:04: 3000000 INFO @ Tue, 14 Jul 2020 08:07:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:07:16: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:07:16: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:07:17: 5000000 INFO @ Tue, 14 Jul 2020 08:07:24: 1000000 INFO @ Tue, 14 Jul 2020 08:07:24: 6000000 INFO @ Tue, 14 Jul 2020 08:07:31: 2000000 INFO @ Tue, 14 Jul 2020 08:07:31: 7000000 INFO @ Tue, 14 Jul 2020 08:07:38: 3000000 INFO @ Tue, 14 Jul 2020 08:07:39: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:07:45: 4000000 INFO @ Tue, 14 Jul 2020 08:07:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:07:46: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:07:46: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:07:46: 9000000 INFO @ Tue, 14 Jul 2020 08:07:50: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:07:50: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:07:50: #1 total tags in treatment: 9518793 INFO @ Tue, 14 Jul 2020 08:07:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:50: #1 tags after filtering in treatment: 9518276 INFO @ Tue, 14 Jul 2020 08:07:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:07:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:07:52: #2 number of paired peaks: 3982 INFO @ Tue, 14 Jul 2020 08:07:52: start model_add_line... INFO @ Tue, 14 Jul 2020 08:07:52: start X-correlation... Traceback (most recent call last): File "/usr/local/bin/macs2", line 4, in __import__('pkg_resources').run_script('MACS2==2.1.1.20160309', 'macs2') File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 719, in run_script File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 1511, in run_script File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 617, in File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 57, in main File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/MACS2/callpeak_cmd.py", line 178, in run File "MACS2/PeakModel.pyx", line 108, in MACS2.PeakModel.PeakModel.__init__ (MACS2/PeakModel.c:2438) File "MACS2/PeakModel.pyx", line 150, in MACS2.PeakModel.PeakModel.build (MACS2/PeakModel.c:3010) File "MACS2/PeakModel.pyx", line 230, in MACS2.PeakModel.PeakModel.__paired_peak_model (MACS2/PeakModel.c:4266) ValueError: max() arg is an empty sequence cut: /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:07:53: 5000000 INFO @ Tue, 14 Jul 2020 08:07:54: 1000000 INFO @ Tue, 14 Jul 2020 08:08:00: 6000000 INFO @ Tue, 14 Jul 2020 08:08:01: 2000000 INFO @ Tue, 14 Jul 2020 08:08:07: 7000000 INFO @ Tue, 14 Jul 2020 08:08:08: 3000000 INFO @ Tue, 14 Jul 2020 08:08:14: 8000000 INFO @ Tue, 14 Jul 2020 08:08:15: 4000000 INFO @ Tue, 14 Jul 2020 08:08:20: 9000000 INFO @ Tue, 14 Jul 2020 08:08:21: 5000000 INFO @ Tue, 14 Jul 2020 08:08:24: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:08:24: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:08:24: #1 total tags in treatment: 9518793 INFO @ Tue, 14 Jul 2020 08:08:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:08:24: #1 tags after filtering in treatment: 9518276 INFO @ Tue, 14 Jul 2020 08:08:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:08:24: #1 finished! INFO @ Tue, 14 Jul 2020 08:08:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:08:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:08:25: #2 number of paired peaks: 3982 INFO @ Tue, 14 Jul 2020 08:08:25: start model_add_line... INFO @ Tue, 14 Jul 2020 08:08:25: start X-correlation... Traceback (most recent call last): File "/usr/local/bin/macs2", line 4, in __import__('pkg_resources').run_script('MACS2==2.1.1.20160309', 'macs2') File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 719, in run_script File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 1511, in run_script File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 617, in File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 57, in main File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/MACS2/callpeak_cmd.py", line 178, in run File "MACS2/PeakModel.pyx", line 108, in MACS2.PeakModel.PeakModel.__init__ (MACS2/PeakModel.c:2438) File "MACS2/PeakModel.pyx", line 150, in MACS2.PeakModel.PeakModel.build (MACS2/PeakModel.c:3010) File "MACS2/PeakModel.pyx", line 230, in MACS2.PeakModel.PeakModel.__paired_peak_model (MACS2/PeakModel.c:4266) ValueError: max() arg is an empty sequence cut: /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:08:28: 6000000 INFO @ Tue, 14 Jul 2020 08:08:34: 7000000 INFO @ Tue, 14 Jul 2020 08:08:40: 8000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:08:47: 9000000 INFO @ Tue, 14 Jul 2020 08:08:50: #1 tag size is determined as 78 bps INFO @ Tue, 14 Jul 2020 08:08:50: #1 tag size = 78 INFO @ Tue, 14 Jul 2020 08:08:50: #1 total tags in treatment: 9518793 INFO @ Tue, 14 Jul 2020 08:08:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:08:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:08:50: #1 tags after filtering in treatment: 9518276 INFO @ Tue, 14 Jul 2020 08:08:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:08:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:08:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:08:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:08:51: #2 number of paired peaks: 3982 INFO @ Tue, 14 Jul 2020 08:08:51: start model_add_line... INFO @ Tue, 14 Jul 2020 08:08:52: start X-correlation... Traceback (most recent call last): File "/usr/local/bin/macs2", line 4, in __import__('pkg_resources').run_script('MACS2==2.1.1.20160309', 'macs2') File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 719, in run_script File "/usr/local/lib/python2.7/site-packages/setuptools-20.7.0-py2.7.egg/pkg_resources/__init__.py", line 1511, in run_script File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 617, in File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/EGG-INFO/scripts/macs2", line 57, in main File "/usr/local/lib/python2.7/site-packages/MACS2-2.1.1.20160309-py2.7-linux-x86_64.egg/MACS2/callpeak_cmd.py", line 178, in run File "MACS2/PeakModel.pyx", line 108, in MACS2.PeakModel.PeakModel.__init__ (MACS2/PeakModel.c:2438) File "MACS2/PeakModel.pyx", line 150, in MACS2.PeakModel.PeakModel.build (MACS2/PeakModel.c:3010) File "MACS2/PeakModel.pyx", line 230, in MACS2.PeakModel.PeakModel.__paired_peak_model (MACS2/PeakModel.c:4266) ValueError: max() arg is an empty sequence cut: /home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5734952/SRX5734952.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling