Job ID = 6458878 SRX = SRX5717047 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:42:57 prefetch.2.10.7: 1) Downloading 'SRR8936408'... 2020-06-21T12:42:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:48:03 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:48:03 prefetch.2.10.7: 1) 'SRR8936408' was downloaded successfully Read 29934211 spots for SRR8936408/SRR8936408.sra Written 29934211 spots for SRR8936408/SRR8936408.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 29934211 reads; of these: 29934211 (100.00%) were unpaired; of these: 3107015 (10.38%) aligned 0 times 20546695 (68.64%) aligned exactly 1 time 6280501 (20.98%) aligned >1 times 89.62% overall alignment rate Time searching: 00:07:22 Overall time: 00:07:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8256791 / 26827196 = 0.3078 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:02:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:02:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:02:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:02:15: 1000000 INFO @ Sun, 21 Jun 2020 22:02:20: 2000000 INFO @ Sun, 21 Jun 2020 22:02:25: 3000000 INFO @ Sun, 21 Jun 2020 22:02:30: 4000000 INFO @ Sun, 21 Jun 2020 22:02:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:02:40: 6000000 INFO @ Sun, 21 Jun 2020 22:02:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:02:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:02:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:02:45: 7000000 INFO @ Sun, 21 Jun 2020 22:02:45: 1000000 INFO @ Sun, 21 Jun 2020 22:02:50: 8000000 INFO @ Sun, 21 Jun 2020 22:02:50: 2000000 INFO @ Sun, 21 Jun 2020 22:02:56: 9000000 INFO @ Sun, 21 Jun 2020 22:02:56: 3000000 INFO @ Sun, 21 Jun 2020 22:03:01: 10000000 INFO @ Sun, 21 Jun 2020 22:03:01: 4000000 INFO @ Sun, 21 Jun 2020 22:03:06: 5000000 INFO @ Sun, 21 Jun 2020 22:03:07: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:12: 6000000 INFO @ Sun, 21 Jun 2020 22:03:12: 12000000 INFO @ Sun, 21 Jun 2020 22:03:15: 1000000 INFO @ Sun, 21 Jun 2020 22:03:17: 7000000 INFO @ Sun, 21 Jun 2020 22:03:18: 13000000 INFO @ Sun, 21 Jun 2020 22:03:21: 2000000 INFO @ Sun, 21 Jun 2020 22:03:23: 8000000 INFO @ Sun, 21 Jun 2020 22:03:23: 14000000 INFO @ Sun, 21 Jun 2020 22:03:26: 3000000 INFO @ Sun, 21 Jun 2020 22:03:28: 9000000 INFO @ Sun, 21 Jun 2020 22:03:29: 15000000 INFO @ Sun, 21 Jun 2020 22:03:32: 4000000 INFO @ Sun, 21 Jun 2020 22:03:33: 10000000 INFO @ Sun, 21 Jun 2020 22:03:35: 16000000 INFO @ Sun, 21 Jun 2020 22:03:37: 5000000 INFO @ Sun, 21 Jun 2020 22:03:39: 11000000 INFO @ Sun, 21 Jun 2020 22:03:40: 17000000 INFO @ Sun, 21 Jun 2020 22:03:43: 6000000 INFO @ Sun, 21 Jun 2020 22:03:44: 12000000 INFO @ Sun, 21 Jun 2020 22:03:46: 18000000 INFO @ Sun, 21 Jun 2020 22:03:48: 7000000 INFO @ Sun, 21 Jun 2020 22:03:50: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:03:50: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:03:50: #1 total tags in treatment: 18570405 INFO @ Sun, 21 Jun 2020 22:03:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:03:50: 13000000 INFO @ Sun, 21 Jun 2020 22:03:50: #1 tags after filtering in treatment: 18570340 INFO @ Sun, 21 Jun 2020 22:03:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:03:50: #1 finished! INFO @ Sun, 21 Jun 2020 22:03:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:03:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:03:51: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 22:03:51: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:03:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:03:53: 8000000 INFO @ Sun, 21 Jun 2020 22:03:55: 14000000 INFO @ Sun, 21 Jun 2020 22:03:59: 9000000 INFO @ Sun, 21 Jun 2020 22:04:01: 15000000 INFO @ Sun, 21 Jun 2020 22:04:04: 10000000 INFO @ Sun, 21 Jun 2020 22:04:07: 16000000 INFO @ Sun, 21 Jun 2020 22:04:10: 11000000 INFO @ Sun, 21 Jun 2020 22:04:12: 17000000 INFO @ Sun, 21 Jun 2020 22:04:15: 12000000 INFO @ Sun, 21 Jun 2020 22:04:18: 18000000 INFO @ Sun, 21 Jun 2020 22:04:21: 13000000 INFO @ Sun, 21 Jun 2020 22:04:21: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:04:21: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:04:21: #1 total tags in treatment: 18570405 INFO @ Sun, 21 Jun 2020 22:04:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:22: #1 tags after filtering in treatment: 18570340 INFO @ Sun, 21 Jun 2020 22:04:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:22: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:23: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 22:04:23: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:04:26: 14000000 INFO @ Sun, 21 Jun 2020 22:04:31: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:04:37: 16000000 INFO @ Sun, 21 Jun 2020 22:04:43: 17000000 INFO @ Sun, 21 Jun 2020 22:04:48: 18000000 INFO @ Sun, 21 Jun 2020 22:04:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:04:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:04:51: #1 total tags in treatment: 18570405 INFO @ Sun, 21 Jun 2020 22:04:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:52: #1 tags after filtering in treatment: 18570340 INFO @ Sun, 21 Jun 2020 22:04:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:52: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:53: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 22:04:53: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5717047/SRX5717047.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。