Job ID = 6458863 SRX = SRX5717034 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:43:07 prefetch.2.10.7: 1) Downloading 'SRR8936394'... 2020-06-21T12:43:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:45:17 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:45:17 prefetch.2.10.7: 1) 'SRR8936394' was downloaded successfully Read 25164570 spots for SRR8936394/SRR8936394.sra Written 25164570 spots for SRR8936394/SRR8936394.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:03 25164570 reads; of these: 25164570 (100.00%) were unpaired; of these: 2498848 (9.93%) aligned 0 times 17580772 (69.86%) aligned exactly 1 time 5084950 (20.21%) aligned >1 times 90.07% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8327097 / 22665722 = 0.3674 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:56:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:56:55: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:56:55: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:57:02: 1000000 INFO @ Sun, 21 Jun 2020 21:57:08: 2000000 INFO @ Sun, 21 Jun 2020 21:57:14: 3000000 INFO @ Sun, 21 Jun 2020 21:57:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:25: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:25: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:57:27: 5000000 INFO @ Sun, 21 Jun 2020 21:57:33: 1000000 INFO @ Sun, 21 Jun 2020 21:57:34: 6000000 INFO @ Sun, 21 Jun 2020 21:57:39: 2000000 INFO @ Sun, 21 Jun 2020 21:57:41: 7000000 INFO @ Sun, 21 Jun 2020 21:57:46: 3000000 INFO @ Sun, 21 Jun 2020 21:57:48: 8000000 INFO @ Sun, 21 Jun 2020 21:57:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:55: 9000000 INFO @ Sun, 21 Jun 2020 21:57:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:00: 5000000 INFO @ Sun, 21 Jun 2020 21:58:02: 10000000 INFO @ Sun, 21 Jun 2020 21:58:03: 1000000 INFO @ Sun, 21 Jun 2020 21:58:07: 6000000 INFO @ Sun, 21 Jun 2020 21:58:09: 11000000 INFO @ Sun, 21 Jun 2020 21:58:10: 2000000 INFO @ Sun, 21 Jun 2020 21:58:15: 7000000 INFO @ Sun, 21 Jun 2020 21:58:16: 12000000 INFO @ Sun, 21 Jun 2020 21:58:17: 3000000 INFO @ Sun, 21 Jun 2020 21:58:22: 8000000 INFO @ Sun, 21 Jun 2020 21:58:23: 13000000 INFO @ Sun, 21 Jun 2020 21:58:24: 4000000 INFO @ Sun, 21 Jun 2020 21:58:29: 9000000 INFO @ Sun, 21 Jun 2020 21:58:30: 14000000 INFO @ Sun, 21 Jun 2020 21:58:31: 5000000 INFO @ Sun, 21 Jun 2020 21:58:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:58:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:58:33: #1 total tags in treatment: 14338625 INFO @ Sun, 21 Jun 2020 21:58:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:58:33: #1 tags after filtering in treatment: 14338511 INFO @ Sun, 21 Jun 2020 21:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:58:33: #1 finished! INFO @ Sun, 21 Jun 2020 21:58:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:58:34: #2 number of paired peaks: 1927 INFO @ Sun, 21 Jun 2020 21:58:34: start model_add_line... INFO @ Sun, 21 Jun 2020 21:58:35: start X-correlation... INFO @ Sun, 21 Jun 2020 21:58:35: end of X-cor INFO @ Sun, 21 Jun 2020 21:58:35: #2 finished! INFO @ Sun, 21 Jun 2020 21:58:35: #2 predicted fragment length is 0 bps INFO @ Sun, 21 Jun 2020 21:58:35: #2 alternative fragment length(s) may be 0,46,100,130,170,529,598 bps INFO @ Sun, 21 Jun 2020 21:58:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.05_model.r WARNING @ Sun, 21 Jun 2020 21:58:35: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 21:58:35: #2 You may need to consider one of the other alternative d(s): 0,46,100,130,170,529,598 WARNING @ Sun, 21 Jun 2020 21:58:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 21:58:35: #3 Call peaks... INFO @ Sun, 21 Jun 2020 21:58:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 21:58:36: 10000000 INFO @ Sun, 21 Jun 2020 21:58:38: 6000000 INFO @ Sun, 21 Jun 2020 21:58:43: 11000000 INFO @ Sun, 21 Jun 2020 21:58:45: 7000000 INFO @ Sun, 21 Jun 2020 21:58:50: 12000000 INFO @ Sun, 21 Jun 2020 21:58:52: 8000000 INFO @ Sun, 21 Jun 2020 21:58:57: 13000000 INFO @ Sun, 21 Jun 2020 21:58:59: 9000000 INFO @ Sun, 21 Jun 2020 21:59:04: 14000000 INFO @ Sun, 21 Jun 2020 21:59:07: 10000000 INFO @ Sun, 21 Jun 2020 21:59:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:59:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:59:07: #1 total tags in treatment: 14338625 INFO @ Sun, 21 Jun 2020 21:59:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:59:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:59:07: #1 tags after filtering in treatment: 14338511 INFO @ Sun, 21 Jun 2020 21:59:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:59:07: #1 finished! INFO @ Sun, 21 Jun 2020 21:59:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:59:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:59:08: #2 number of paired peaks: 1927 INFO @ Sun, 21 Jun 2020 21:59:08: start model_add_line... INFO @ Sun, 21 Jun 2020 21:59:09: start X-correlation... INFO @ Sun, 21 Jun 2020 21:59:09: end of X-cor INFO @ Sun, 21 Jun 2020 21:59:09: #2 finished! INFO @ Sun, 21 Jun 2020 21:59:09: #2 predicted fragment length is 0 bps INFO @ Sun, 21 Jun 2020 21:59:09: #2 alternative fragment length(s) may be 0,46,100,130,170,529,598 bps INFO @ Sun, 21 Jun 2020 21:59:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.10_model.r WARNING @ Sun, 21 Jun 2020 21:59:09: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 21:59:09: #2 You may need to consider one of the other alternative d(s): 0,46,100,130,170,529,598 WARNING @ Sun, 21 Jun 2020 21:59:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 21:59:09: #3 Call peaks... INFO @ Sun, 21 Jun 2020 21:59:09: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:59:13: 11000000 INFO @ Sun, 21 Jun 2020 21:59:20: 12000000 INFO @ Sun, 21 Jun 2020 21:59:27: 13000000 INFO @ Sun, 21 Jun 2020 21:59:33: 14000000 INFO @ Sun, 21 Jun 2020 21:59:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:59:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:59:36: #1 total tags in treatment: 14338625 INFO @ Sun, 21 Jun 2020 21:59:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:59:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:59:36: #1 tags after filtering in treatment: 14338511 INFO @ Sun, 21 Jun 2020 21:59:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:59:36: #1 finished! INFO @ Sun, 21 Jun 2020 21:59:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:59:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:59:37: #2 number of paired peaks: 1927 INFO @ Sun, 21 Jun 2020 21:59:37: start model_add_line... INFO @ Sun, 21 Jun 2020 21:59:37: start X-correlation... INFO @ Sun, 21 Jun 2020 21:59:37: end of X-cor INFO @ Sun, 21 Jun 2020 21:59:37: #2 finished! INFO @ Sun, 21 Jun 2020 21:59:37: #2 predicted fragment length is 0 bps INFO @ Sun, 21 Jun 2020 21:59:37: #2 alternative fragment length(s) may be 0,46,100,130,170,529,598 bps INFO @ Sun, 21 Jun 2020 21:59:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5717034/SRX5717034.20_model.r WARNING @ Sun, 21 Jun 2020 21:59:37: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 21:59:37: #2 You may need to consider one of the other alternative d(s): 0,46,100,130,170,529,598 WARNING @ Sun, 21 Jun 2020 21:59:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 21:59:37: #3 Call peaks... INFO @ Sun, 21 Jun 2020 21:59:37: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 /var/spool/uge/at150/job_scripts/6458863: line 273: 22051 Terminated MACS $i /var/spool/uge/at150/job_scripts/6458863: line 273: 34687 Terminated MACS $i /var/spool/uge/at150/job_scripts/6458863: line 273: 35049 Terminated MACS $i ls: cannot access SRX5717034.05.bed: No such file or directory mv: cannot stat ‘SRX5717034.05.bed’: No such file or directory mv: cannot stat ‘SRX5717034.05.bb’: No such file or directory ls: cannot access SRX5717034.10.bed: No such file or directory mv: cannot stat ‘SRX5717034.10.bed’: No such file or directory mv: cannot stat ‘SRX5717034.10.bb’: No such file or directory ls: cannot access SRX5717034.20.bed: No such file or directory mv: cannot stat ‘SRX5717034.20.bed’: No such file or directory mv: cannot stat ‘SRX5717034.20.bb’: No such file or directory