Job ID = 6529981 SRX = SRX5717033 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:34 25658952 reads; of these: 25658952 (100.00%) were unpaired; of these: 2912324 (11.35%) aligned 0 times 17774524 (69.27%) aligned exactly 1 time 4972104 (19.38%) aligned >1 times 88.65% overall alignment rate Time searching: 00:06:34 Overall time: 00:06:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8524081 / 22746628 = 0.3747 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:15:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:15:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:15:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:15:47: 1000000 INFO @ Tue, 30 Jun 2020 03:15:53: 2000000 INFO @ Tue, 30 Jun 2020 03:15:59: 3000000 INFO @ Tue, 30 Jun 2020 03:16:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:16:11: 5000000 INFO @ Tue, 30 Jun 2020 03:16:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:16:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:16:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:16:17: 6000000 INFO @ Tue, 30 Jun 2020 03:16:19: 1000000 INFO @ Tue, 30 Jun 2020 03:16:24: 7000000 INFO @ Tue, 30 Jun 2020 03:16:25: 2000000 INFO @ Tue, 30 Jun 2020 03:16:31: 8000000 INFO @ Tue, 30 Jun 2020 03:16:31: 3000000 INFO @ Tue, 30 Jun 2020 03:16:37: 9000000 INFO @ Tue, 30 Jun 2020 03:16:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:16:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:16:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:16:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:16:44: 10000000 INFO @ Tue, 30 Jun 2020 03:16:44: 5000000 INFO @ Tue, 30 Jun 2020 03:16:49: 1000000 INFO @ Tue, 30 Jun 2020 03:16:51: 11000000 INFO @ Tue, 30 Jun 2020 03:16:51: 6000000 INFO @ Tue, 30 Jun 2020 03:16:57: 2000000 INFO @ Tue, 30 Jun 2020 03:16:58: 12000000 INFO @ Tue, 30 Jun 2020 03:16:58: 7000000 INFO @ Tue, 30 Jun 2020 03:17:04: 13000000 INFO @ Tue, 30 Jun 2020 03:17:05: 8000000 INFO @ Tue, 30 Jun 2020 03:17:05: 3000000 INFO @ Tue, 30 Jun 2020 03:17:11: 14000000 INFO @ Tue, 30 Jun 2020 03:17:12: 9000000 INFO @ Tue, 30 Jun 2020 03:17:12: 4000000 INFO @ Tue, 30 Jun 2020 03:17:13: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:17:13: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:17:13: #1 total tags in treatment: 14222547 INFO @ Tue, 30 Jun 2020 03:17:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:17:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:17:14: #1 tags after filtering in treatment: 14222461 INFO @ Tue, 30 Jun 2020 03:17:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:17:14: #1 finished! INFO @ Tue, 30 Jun 2020 03:17:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:17:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:17:15: #2 number of paired peaks: 2431 INFO @ Tue, 30 Jun 2020 03:17:15: start model_add_line... INFO @ Tue, 30 Jun 2020 03:17:15: start X-correlation... INFO @ Tue, 30 Jun 2020 03:17:15: end of X-cor INFO @ Tue, 30 Jun 2020 03:17:15: #2 finished! INFO @ Tue, 30 Jun 2020 03:17:15: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 03:17:15: #2 alternative fragment length(s) may be 0,12,59,118,145,150,166,197,227,285,529 bps INFO @ Tue, 30 Jun 2020 03:17:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.05_model.r WARNING @ Tue, 30 Jun 2020 03:17:15: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:17:15: #2 You may need to consider one of the other alternative d(s): 0,12,59,118,145,150,166,197,227,285,529 WARNING @ Tue, 30 Jun 2020 03:17:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:17:15: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:17:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 30 Jun 2020 03:17:18: 10000000 INFO @ Tue, 30 Jun 2020 03:17:20: 5000000 INFO @ Tue, 30 Jun 2020 03:17:25: 11000000 INFO @ Tue, 30 Jun 2020 03:17:27: 6000000 INFO @ Tue, 30 Jun 2020 03:17:31: 12000000 INFO @ Tue, 30 Jun 2020 03:17:35: 7000000 INFO @ Tue, 30 Jun 2020 03:17:38: 13000000 INFO @ Tue, 30 Jun 2020 03:17:42: 8000000 INFO @ Tue, 30 Jun 2020 03:17:44: 14000000 INFO @ Tue, 30 Jun 2020 03:17:46: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:17:46: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:17:46: #1 total tags in treatment: 14222547 INFO @ Tue, 30 Jun 2020 03:17:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:17:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:17:47: #1 tags after filtering in treatment: 14222461 INFO @ Tue, 30 Jun 2020 03:17:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:17:47: #1 finished! INFO @ Tue, 30 Jun 2020 03:17:47: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:17:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:17:48: #2 number of paired peaks: 2431 INFO @ Tue, 30 Jun 2020 03:17:48: start model_add_line... INFO @ Tue, 30 Jun 2020 03:17:48: start X-correlation... INFO @ Tue, 30 Jun 2020 03:17:48: end of X-cor INFO @ Tue, 30 Jun 2020 03:17:48: #2 finished! INFO @ Tue, 30 Jun 2020 03:17:48: #2 predicted fragment length is 0 bps INFO @ Tue, 30 Jun 2020 03:17:48: #2 alternative fragment length(s) may be 0,12,59,118,145,150,166,197,227,285,529 bps INFO @ Tue, 30 Jun 2020 03:17:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5717033/SRX5717033.10_model.r WARNING @ Tue, 30 Jun 2020 03:17:48: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 30 Jun 2020 03:17:48: #2 You may need to consider one of the other alternative d(s): 0,12,59,118,145,150,166,197,227,285,529 WARNING @ Tue, 30 Jun 2020 03:17:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 30 Jun 2020 03:17:48: #3 Call peaks... INFO @ Tue, 30 Jun 2020 03:17:48: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 03:17:49: 9000000 INFO @ Tue, 30 Jun 2020 03:17:57: 10000000 INFO @ Tue, 30 Jun 2020 03:18:03: 11000000 INFO @ Tue, 30 Jun 2020 03:18:10: 12000000 INFO @ Tue, 30 Jun 2020 03:18:17: 13000000 BigWig に変換しました。 /var/spool/uge/at157/job_scripts/6529981: line 293: 22377 Terminated MACS $i /var/spool/uge/at157/job_scripts/6529981: line 293: 32006 Terminated MACS $i /var/spool/uge/at157/job_scripts/6529981: line 293: 51466 Terminated MACS $i ls: cannot access SRX5717033.05.bed: No such file or directory mv: cannot stat ‘SRX5717033.05.bed’: No such file or directory mv: cannot stat ‘SRX5717033.05.bb’: No such file or directory ls: cannot access SRX5717033.10.bed: No such file or directory mv: cannot stat ‘SRX5717033.10.bed’: No such file or directory mv: cannot stat ‘SRX5717033.10.bb’: No such file or directory ls: cannot access SRX5717033.20.bed: No such file or directory mv: cannot stat ‘SRX5717033.20.bed’: No such file or directory mv: cannot stat ‘SRX5717033.20.bb’: No such file or directory