Job ID = 6458838 SRX = SRX5661484 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:37:56 prefetch.2.10.7: 1) Downloading 'SRR8874491'... 2020-06-21T12:37:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:41:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:41:45 prefetch.2.10.7: 1) 'SRR8874491' was downloaded successfully 2020-06-21T12:41:45 prefetch.2.10.7: 'SRR8874491' has 0 unresolved dependencies Read 50603172 spots for SRR8874491/SRR8874491.sra Written 50603172 spots for SRR8874491/SRR8874491.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:47 50603172 reads; of these: 50603172 (100.00%) were unpaired; of these: 1936078 (3.83%) aligned 0 times 36089041 (71.32%) aligned exactly 1 time 12578053 (24.86%) aligned >1 times 96.17% overall alignment rate Time searching: 00:16:47 Overall time: 00:16:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9297699 / 48667094 = 0.1910 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:10:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:10:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:10:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:10:25: 1000000 INFO @ Sun, 21 Jun 2020 22:10:30: 2000000 INFO @ Sun, 21 Jun 2020 22:10:35: 3000000 INFO @ Sun, 21 Jun 2020 22:10:41: 4000000 INFO @ Sun, 21 Jun 2020 22:10:46: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:10:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:10:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:10:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:10:52: 6000000 INFO @ Sun, 21 Jun 2020 22:10:55: 1000000 INFO @ Sun, 21 Jun 2020 22:10:58: 7000000 INFO @ Sun, 21 Jun 2020 22:11:01: 2000000 INFO @ Sun, 21 Jun 2020 22:11:03: 8000000 INFO @ Sun, 21 Jun 2020 22:11:07: 3000000 INFO @ Sun, 21 Jun 2020 22:11:09: 9000000 INFO @ Sun, 21 Jun 2020 22:11:12: 4000000 INFO @ Sun, 21 Jun 2020 22:11:15: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:11:18: 5000000 INFO @ Sun, 21 Jun 2020 22:11:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:11:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:11:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:11:21: 11000000 INFO @ Sun, 21 Jun 2020 22:11:24: 6000000 INFO @ Sun, 21 Jun 2020 22:11:25: 1000000 INFO @ Sun, 21 Jun 2020 22:11:27: 12000000 INFO @ Sun, 21 Jun 2020 22:11:30: 7000000 INFO @ Sun, 21 Jun 2020 22:11:31: 2000000 INFO @ Sun, 21 Jun 2020 22:11:32: 13000000 INFO @ Sun, 21 Jun 2020 22:11:35: 8000000 INFO @ Sun, 21 Jun 2020 22:11:37: 3000000 INFO @ Sun, 21 Jun 2020 22:11:38: 14000000 INFO @ Sun, 21 Jun 2020 22:11:41: 9000000 INFO @ Sun, 21 Jun 2020 22:11:43: 4000000 INFO @ Sun, 21 Jun 2020 22:11:44: 15000000 INFO @ Sun, 21 Jun 2020 22:11:47: 10000000 INFO @ Sun, 21 Jun 2020 22:11:48: 5000000 INFO @ Sun, 21 Jun 2020 22:11:50: 16000000 INFO @ Sun, 21 Jun 2020 22:11:53: 11000000 INFO @ Sun, 21 Jun 2020 22:11:54: 6000000 INFO @ Sun, 21 Jun 2020 22:11:56: 17000000 INFO @ Sun, 21 Jun 2020 22:11:59: 12000000 INFO @ Sun, 21 Jun 2020 22:12:00: 7000000 INFO @ Sun, 21 Jun 2020 22:12:02: 18000000 INFO @ Sun, 21 Jun 2020 22:12:04: 13000000 INFO @ Sun, 21 Jun 2020 22:12:06: 8000000 INFO @ Sun, 21 Jun 2020 22:12:08: 19000000 INFO @ Sun, 21 Jun 2020 22:12:10: 14000000 INFO @ Sun, 21 Jun 2020 22:12:11: 9000000 INFO @ Sun, 21 Jun 2020 22:12:13: 20000000 INFO @ Sun, 21 Jun 2020 22:12:16: 15000000 INFO @ Sun, 21 Jun 2020 22:12:17: 10000000 INFO @ Sun, 21 Jun 2020 22:12:19: 21000000 INFO @ Sun, 21 Jun 2020 22:12:22: 16000000 INFO @ Sun, 21 Jun 2020 22:12:23: 11000000 INFO @ Sun, 21 Jun 2020 22:12:25: 22000000 INFO @ Sun, 21 Jun 2020 22:12:28: 17000000 INFO @ Sun, 21 Jun 2020 22:12:29: 12000000 INFO @ Sun, 21 Jun 2020 22:12:31: 23000000 INFO @ Sun, 21 Jun 2020 22:12:34: 18000000 INFO @ Sun, 21 Jun 2020 22:12:34: 13000000 INFO @ Sun, 21 Jun 2020 22:12:37: 24000000 INFO @ Sun, 21 Jun 2020 22:12:39: 19000000 INFO @ Sun, 21 Jun 2020 22:12:40: 14000000 INFO @ Sun, 21 Jun 2020 22:12:42: 25000000 INFO @ Sun, 21 Jun 2020 22:12:45: 20000000 INFO @ Sun, 21 Jun 2020 22:12:46: 15000000 INFO @ Sun, 21 Jun 2020 22:12:48: 26000000 INFO @ Sun, 21 Jun 2020 22:12:51: 21000000 INFO @ Sun, 21 Jun 2020 22:12:52: 16000000 INFO @ Sun, 21 Jun 2020 22:12:54: 27000000 INFO @ Sun, 21 Jun 2020 22:12:57: 22000000 INFO @ Sun, 21 Jun 2020 22:12:57: 17000000 INFO @ Sun, 21 Jun 2020 22:13:00: 28000000 INFO @ Sun, 21 Jun 2020 22:13:03: 23000000 INFO @ Sun, 21 Jun 2020 22:13:03: 18000000 INFO @ Sun, 21 Jun 2020 22:13:06: 29000000 INFO @ Sun, 21 Jun 2020 22:13:08: 24000000 INFO @ Sun, 21 Jun 2020 22:13:09: 19000000 INFO @ Sun, 21 Jun 2020 22:13:11: 30000000 INFO @ Sun, 21 Jun 2020 22:13:14: 25000000 INFO @ Sun, 21 Jun 2020 22:13:15: 20000000 INFO @ Sun, 21 Jun 2020 22:13:17: 31000000 INFO @ Sun, 21 Jun 2020 22:13:20: 26000000 INFO @ Sun, 21 Jun 2020 22:13:21: 21000000 INFO @ Sun, 21 Jun 2020 22:13:23: 32000000 INFO @ Sun, 21 Jun 2020 22:13:26: 27000000 INFO @ Sun, 21 Jun 2020 22:13:27: 22000000 INFO @ Sun, 21 Jun 2020 22:13:29: 33000000 INFO @ Sun, 21 Jun 2020 22:13:32: 28000000 INFO @ Sun, 21 Jun 2020 22:13:33: 23000000 INFO @ Sun, 21 Jun 2020 22:13:35: 34000000 INFO @ Sun, 21 Jun 2020 22:13:38: 29000000 INFO @ Sun, 21 Jun 2020 22:13:38: 24000000 INFO @ Sun, 21 Jun 2020 22:13:41: 35000000 INFO @ Sun, 21 Jun 2020 22:13:43: 30000000 INFO @ Sun, 21 Jun 2020 22:13:44: 25000000 INFO @ Sun, 21 Jun 2020 22:13:47: 36000000 INFO @ Sun, 21 Jun 2020 22:13:49: 31000000 INFO @ Sun, 21 Jun 2020 22:13:50: 26000000 INFO @ Sun, 21 Jun 2020 22:13:53: 37000000 INFO @ Sun, 21 Jun 2020 22:13:55: 32000000 INFO @ Sun, 21 Jun 2020 22:13:56: 27000000 INFO @ Sun, 21 Jun 2020 22:13:58: 38000000 INFO @ Sun, 21 Jun 2020 22:14:01: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:14:02: 28000000 INFO @ Sun, 21 Jun 2020 22:14:04: 39000000 INFO @ Sun, 21 Jun 2020 22:14:07: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:14:07: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:14:07: #1 total tags in treatment: 39369395 INFO @ Sun, 21 Jun 2020 22:14:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:14:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:14:07: 34000000 INFO @ Sun, 21 Jun 2020 22:14:08: 29000000 INFO @ Sun, 21 Jun 2020 22:14:08: #1 tags after filtering in treatment: 39369394 INFO @ Sun, 21 Jun 2020 22:14:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:14:08: #1 finished! INFO @ Sun, 21 Jun 2020 22:14:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:14:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:14:10: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 22:14:10: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:14:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:14:13: 35000000 INFO @ Sun, 21 Jun 2020 22:14:13: 30000000 INFO @ Sun, 21 Jun 2020 22:14:19: 36000000 INFO @ Sun, 21 Jun 2020 22:14:19: 31000000 INFO @ Sun, 21 Jun 2020 22:14:24: 37000000 INFO @ Sun, 21 Jun 2020 22:14:25: 32000000 INFO @ Sun, 21 Jun 2020 22:14:30: 38000000 INFO @ Sun, 21 Jun 2020 22:14:31: 33000000 INFO @ Sun, 21 Jun 2020 22:14:36: 39000000 INFO @ Sun, 21 Jun 2020 22:14:37: 34000000 INFO @ Sun, 21 Jun 2020 22:14:38: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:14:38: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:14:38: #1 total tags in treatment: 39369395 INFO @ Sun, 21 Jun 2020 22:14:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:14:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:14:39: #1 tags after filtering in treatment: 39369394 INFO @ Sun, 21 Jun 2020 22:14:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:14:39: #1 finished! INFO @ Sun, 21 Jun 2020 22:14:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:14:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:14:42: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 22:14:42: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:14:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:14:43: 35000000 INFO @ Sun, 21 Jun 2020 22:14:48: 36000000 INFO @ Sun, 21 Jun 2020 22:14:54: 37000000 INFO @ Sun, 21 Jun 2020 22:14:59: 38000000 INFO @ Sun, 21 Jun 2020 22:15:04: 39000000 INFO @ Sun, 21 Jun 2020 22:15:07: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:15:07: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:15:07: #1 total tags in treatment: 39369395 INFO @ Sun, 21 Jun 2020 22:15:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:15:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:15:07: #1 tags after filtering in treatment: 39369394 INFO @ Sun, 21 Jun 2020 22:15:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:15:07: #1 finished! INFO @ Sun, 21 Jun 2020 22:15:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:15:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:15:10: #2 number of paired peaks: 48 WARNING @ Sun, 21 Jun 2020 22:15:10: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:15:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661484/SRX5661484.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。