Job ID = 6458836 SRX = SRX5661482 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:37:26 prefetch.2.10.7: 1) Downloading 'SRR8874489'... 2020-06-21T12:37:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:44:16 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:44:16 prefetch.2.10.7: 1) 'SRR8874489' was downloaded successfully 2020-06-21T12:44:16 prefetch.2.10.7: 'SRR8874489' has 0 unresolved dependencies Read 76006596 spots for SRR8874489/SRR8874489.sra Written 76006596 spots for SRR8874489/SRR8874489.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:54 76006596 reads; of these: 76006596 (100.00%) were unpaired; of these: 2610194 (3.43%) aligned 0 times 53334291 (70.17%) aligned exactly 1 time 20062111 (26.40%) aligned >1 times 96.57% overall alignment rate Time searching: 00:24:54 Overall time: 00:24:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 17152793 / 73396402 = 0.2337 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:26:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:26:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:26:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:26:33: 1000000 INFO @ Sun, 21 Jun 2020 22:26:38: 2000000 INFO @ Sun, 21 Jun 2020 22:26:43: 3000000 INFO @ Sun, 21 Jun 2020 22:26:48: 4000000 INFO @ Sun, 21 Jun 2020 22:26:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:26:58: 6000000 INFO @ Sun, 21 Jun 2020 22:26:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:26:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:26:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:27:03: 7000000 INFO @ Sun, 21 Jun 2020 22:27:04: 1000000 INFO @ Sun, 21 Jun 2020 22:27:08: 8000000 INFO @ Sun, 21 Jun 2020 22:27:09: 2000000 INFO @ Sun, 21 Jun 2020 22:27:13: 9000000 INFO @ Sun, 21 Jun 2020 22:27:14: 3000000 INFO @ Sun, 21 Jun 2020 22:27:18: 10000000 INFO @ Sun, 21 Jun 2020 22:27:19: 4000000 INFO @ Sun, 21 Jun 2020 22:27:23: 11000000 INFO @ Sun, 21 Jun 2020 22:27:25: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:27:28: 12000000 INFO @ Sun, 21 Jun 2020 22:27:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:27:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:27:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:27:30: 6000000 INFO @ Sun, 21 Jun 2020 22:27:34: 13000000 INFO @ Sun, 21 Jun 2020 22:27:34: 1000000 INFO @ Sun, 21 Jun 2020 22:27:35: 7000000 INFO @ Sun, 21 Jun 2020 22:27:39: 14000000 INFO @ Sun, 21 Jun 2020 22:27:39: 2000000 INFO @ Sun, 21 Jun 2020 22:27:40: 8000000 INFO @ Sun, 21 Jun 2020 22:27:44: 15000000 INFO @ Sun, 21 Jun 2020 22:27:44: 3000000 INFO @ Sun, 21 Jun 2020 22:27:46: 9000000 INFO @ Sun, 21 Jun 2020 22:27:49: 16000000 INFO @ Sun, 21 Jun 2020 22:27:50: 4000000 INFO @ Sun, 21 Jun 2020 22:27:51: 10000000 INFO @ Sun, 21 Jun 2020 22:27:54: 17000000 INFO @ Sun, 21 Jun 2020 22:27:55: 5000000 INFO @ Sun, 21 Jun 2020 22:27:56: 11000000 INFO @ Sun, 21 Jun 2020 22:28:00: 18000000 INFO @ Sun, 21 Jun 2020 22:28:01: 6000000 INFO @ Sun, 21 Jun 2020 22:28:02: 12000000 INFO @ Sun, 21 Jun 2020 22:28:05: 19000000 INFO @ Sun, 21 Jun 2020 22:28:06: 7000000 INFO @ Sun, 21 Jun 2020 22:28:07: 13000000 INFO @ Sun, 21 Jun 2020 22:28:10: 20000000 INFO @ Sun, 21 Jun 2020 22:28:11: 8000000 INFO @ Sun, 21 Jun 2020 22:28:12: 14000000 INFO @ Sun, 21 Jun 2020 22:28:15: 21000000 INFO @ Sun, 21 Jun 2020 22:28:16: 9000000 INFO @ Sun, 21 Jun 2020 22:28:17: 15000000 INFO @ Sun, 21 Jun 2020 22:28:20: 22000000 INFO @ Sun, 21 Jun 2020 22:28:22: 10000000 INFO @ Sun, 21 Jun 2020 22:28:23: 16000000 INFO @ Sun, 21 Jun 2020 22:28:25: 23000000 INFO @ Sun, 21 Jun 2020 22:28:27: 11000000 INFO @ Sun, 21 Jun 2020 22:28:28: 17000000 INFO @ Sun, 21 Jun 2020 22:28:30: 24000000 INFO @ Sun, 21 Jun 2020 22:28:32: 12000000 INFO @ Sun, 21 Jun 2020 22:28:33: 18000000 INFO @ Sun, 21 Jun 2020 22:28:36: 25000000 INFO @ Sun, 21 Jun 2020 22:28:37: 13000000 INFO @ Sun, 21 Jun 2020 22:28:38: 19000000 INFO @ Sun, 21 Jun 2020 22:28:41: 26000000 INFO @ Sun, 21 Jun 2020 22:28:43: 14000000 INFO @ Sun, 21 Jun 2020 22:28:43: 20000000 INFO @ Sun, 21 Jun 2020 22:28:46: 27000000 INFO @ Sun, 21 Jun 2020 22:28:48: 15000000 INFO @ Sun, 21 Jun 2020 22:28:49: 21000000 INFO @ Sun, 21 Jun 2020 22:28:51: 28000000 INFO @ Sun, 21 Jun 2020 22:28:53: 16000000 INFO @ Sun, 21 Jun 2020 22:28:54: 22000000 INFO @ Sun, 21 Jun 2020 22:28:56: 29000000 INFO @ Sun, 21 Jun 2020 22:28:58: 17000000 INFO @ Sun, 21 Jun 2020 22:28:59: 23000000 INFO @ Sun, 21 Jun 2020 22:29:02: 30000000 INFO @ Sun, 21 Jun 2020 22:29:04: 18000000 INFO @ Sun, 21 Jun 2020 22:29:04: 24000000 INFO @ Sun, 21 Jun 2020 22:29:07: 31000000 INFO @ Sun, 21 Jun 2020 22:29:09: 19000000 INFO @ Sun, 21 Jun 2020 22:29:09: 25000000 INFO @ Sun, 21 Jun 2020 22:29:12: 32000000 INFO @ Sun, 21 Jun 2020 22:29:14: 20000000 INFO @ Sun, 21 Jun 2020 22:29:14: 26000000 INFO @ Sun, 21 Jun 2020 22:29:17: 33000000 INFO @ Sun, 21 Jun 2020 22:29:19: 21000000 INFO @ Sun, 21 Jun 2020 22:29:20: 27000000 INFO @ Sun, 21 Jun 2020 22:29:22: 34000000 INFO @ Sun, 21 Jun 2020 22:29:24: 22000000 INFO @ Sun, 21 Jun 2020 22:29:25: 28000000 INFO @ Sun, 21 Jun 2020 22:29:27: 35000000 INFO @ Sun, 21 Jun 2020 22:29:29: 23000000 INFO @ Sun, 21 Jun 2020 22:29:30: 29000000 INFO @ Sun, 21 Jun 2020 22:29:32: 36000000 INFO @ Sun, 21 Jun 2020 22:29:34: 24000000 INFO @ Sun, 21 Jun 2020 22:29:35: 30000000 INFO @ Sun, 21 Jun 2020 22:29:38: 37000000 INFO @ Sun, 21 Jun 2020 22:29:40: 25000000 INFO @ Sun, 21 Jun 2020 22:29:41: 31000000 INFO @ Sun, 21 Jun 2020 22:29:43: 38000000 INFO @ Sun, 21 Jun 2020 22:29:45: 26000000 INFO @ Sun, 21 Jun 2020 22:29:46: 32000000 INFO @ Sun, 21 Jun 2020 22:29:48: 39000000 INFO @ Sun, 21 Jun 2020 22:29:50: 27000000 INFO @ Sun, 21 Jun 2020 22:29:51: 33000000 INFO @ Sun, 21 Jun 2020 22:29:53: 40000000 INFO @ Sun, 21 Jun 2020 22:29:55: 28000000 INFO @ Sun, 21 Jun 2020 22:29:56: 34000000 INFO @ Sun, 21 Jun 2020 22:29:58: 41000000 INFO @ Sun, 21 Jun 2020 22:30:00: 29000000 INFO @ Sun, 21 Jun 2020 22:30:01: 35000000 INFO @ Sun, 21 Jun 2020 22:30:03: 42000000 INFO @ Sun, 21 Jun 2020 22:30:06: 30000000 INFO @ Sun, 21 Jun 2020 22:30:06: 36000000 INFO @ Sun, 21 Jun 2020 22:30:08: 43000000 INFO @ Sun, 21 Jun 2020 22:30:11: 31000000 INFO @ Sun, 21 Jun 2020 22:30:11: 37000000 INFO @ Sun, 21 Jun 2020 22:30:13: 44000000 INFO @ Sun, 21 Jun 2020 22:30:16: 32000000 INFO @ Sun, 21 Jun 2020 22:30:17: 38000000 INFO @ Sun, 21 Jun 2020 22:30:19: 45000000 INFO @ Sun, 21 Jun 2020 22:30:21: 33000000 INFO @ Sun, 21 Jun 2020 22:30:22: 39000000 INFO @ Sun, 21 Jun 2020 22:30:24: 46000000 INFO @ Sun, 21 Jun 2020 22:30:27: 34000000 INFO @ Sun, 21 Jun 2020 22:30:27: 40000000 INFO @ Sun, 21 Jun 2020 22:30:29: 47000000 INFO @ Sun, 21 Jun 2020 22:30:32: 35000000 INFO @ Sun, 21 Jun 2020 22:30:32: 41000000 INFO @ Sun, 21 Jun 2020 22:30:35: 48000000 INFO @ Sun, 21 Jun 2020 22:30:37: 36000000 INFO @ Sun, 21 Jun 2020 22:30:38: 42000000 INFO @ Sun, 21 Jun 2020 22:30:40: 49000000 INFO @ Sun, 21 Jun 2020 22:30:42: 37000000 INFO @ Sun, 21 Jun 2020 22:30:43: 43000000 INFO @ Sun, 21 Jun 2020 22:30:45: 50000000 INFO @ Sun, 21 Jun 2020 22:30:48: 38000000 INFO @ Sun, 21 Jun 2020 22:30:49: 44000000 INFO @ Sun, 21 Jun 2020 22:30:50: 51000000 INFO @ Sun, 21 Jun 2020 22:30:53: 39000000 INFO @ Sun, 21 Jun 2020 22:30:55: 45000000 INFO @ Sun, 21 Jun 2020 22:30:55: 52000000 INFO @ Sun, 21 Jun 2020 22:30:59: 40000000 INFO @ Sun, 21 Jun 2020 22:31:00: 46000000 INFO @ Sun, 21 Jun 2020 22:31:01: 53000000 INFO @ Sun, 21 Jun 2020 22:31:04: 41000000 INFO @ Sun, 21 Jun 2020 22:31:06: 47000000 INFO @ Sun, 21 Jun 2020 22:31:06: 54000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:31:09: 42000000 INFO @ Sun, 21 Jun 2020 22:31:11: 48000000 INFO @ Sun, 21 Jun 2020 22:31:11: 55000000 INFO @ Sun, 21 Jun 2020 22:31:15: 43000000 INFO @ Sun, 21 Jun 2020 22:31:16: 49000000 INFO @ Sun, 21 Jun 2020 22:31:16: 56000000 INFO @ Sun, 21 Jun 2020 22:31:18: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:31:18: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:31:18: #1 total tags in treatment: 56243609 INFO @ Sun, 21 Jun 2020 22:31:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:31:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:31:19: #1 tags after filtering in treatment: 56243608 INFO @ Sun, 21 Jun 2020 22:31:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:31:19: #1 finished! INFO @ Sun, 21 Jun 2020 22:31:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:31:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:31:20: 44000000 INFO @ Sun, 21 Jun 2020 22:31:22: 50000000 INFO @ Sun, 21 Jun 2020 22:31:23: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:31:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:31:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:31:25: 45000000 INFO @ Sun, 21 Jun 2020 22:31:27: 51000000 INFO @ Sun, 21 Jun 2020 22:31:31: 46000000 INFO @ Sun, 21 Jun 2020 22:31:32: 52000000 INFO @ Sun, 21 Jun 2020 22:31:36: 47000000 INFO @ Sun, 21 Jun 2020 22:31:37: 53000000 INFO @ Sun, 21 Jun 2020 22:31:41: 48000000 INFO @ Sun, 21 Jun 2020 22:31:42: 54000000 INFO @ Sun, 21 Jun 2020 22:31:47: 49000000 INFO @ Sun, 21 Jun 2020 22:31:48: 55000000 INFO @ Sun, 21 Jun 2020 22:31:52: 50000000 INFO @ Sun, 21 Jun 2020 22:31:53: 56000000 INFO @ Sun, 21 Jun 2020 22:31:55: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:31:55: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:31:55: #1 total tags in treatment: 56243609 INFO @ Sun, 21 Jun 2020 22:31:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:31:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:31:56: #1 tags after filtering in treatment: 56243608 INFO @ Sun, 21 Jun 2020 22:31:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:31:56: #1 finished! INFO @ Sun, 21 Jun 2020 22:31:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:31:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:31:57: 51000000 INFO @ Sun, 21 Jun 2020 22:32:00: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:32:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:32:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:32:03: 52000000 INFO @ Sun, 21 Jun 2020 22:32:08: 53000000 INFO @ Sun, 21 Jun 2020 22:32:13: 54000000 INFO @ Sun, 21 Jun 2020 22:32:18: 55000000 INFO @ Sun, 21 Jun 2020 22:32:24: 56000000 INFO @ Sun, 21 Jun 2020 22:32:25: #1 tag size is determined as 62 bps INFO @ Sun, 21 Jun 2020 22:32:25: #1 tag size = 62 INFO @ Sun, 21 Jun 2020 22:32:25: #1 total tags in treatment: 56243609 INFO @ Sun, 21 Jun 2020 22:32:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:32:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:32:26: #1 tags after filtering in treatment: 56243608 INFO @ Sun, 21 Jun 2020 22:32:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:32:26: #1 finished! INFO @ Sun, 21 Jun 2020 22:32:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:32:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:32:30: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:32:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:32:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5661482/SRX5661482.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。