Job ID = 6458811 SRX = SRX5535383 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:37:00 prefetch.2.10.7: 1) Downloading 'SRR8743311'... 2020-06-21T12:37:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:43:36 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:43:36 prefetch.2.10.7: 1) 'SRR8743311' was downloaded successfully Read 39578038 spots for SRR8743311/SRR8743311.sra Written 39578038 spots for SRR8743311/SRR8743311.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:10 39578038 reads; of these: 39578038 (100.00%) were unpaired; of these: 2108996 (5.33%) aligned 0 times 25101426 (63.42%) aligned exactly 1 time 12367616 (31.25%) aligned >1 times 94.67% overall alignment rate Time searching: 00:11:10 Overall time: 00:11:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14365659 / 37469042 = 0.3834 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:02:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:02:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:02:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:02:59: 1000000 INFO @ Sun, 21 Jun 2020 22:03:05: 2000000 INFO @ Sun, 21 Jun 2020 22:03:10: 3000000 INFO @ Sun, 21 Jun 2020 22:03:16: 4000000 INFO @ Sun, 21 Jun 2020 22:03:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:26: 6000000 INFO @ Sun, 21 Jun 2020 22:03:30: 1000000 INFO @ Sun, 21 Jun 2020 22:03:32: 7000000 INFO @ Sun, 21 Jun 2020 22:03:35: 2000000 INFO @ Sun, 21 Jun 2020 22:03:37: 8000000 INFO @ Sun, 21 Jun 2020 22:03:41: 3000000 INFO @ Sun, 21 Jun 2020 22:03:43: 9000000 INFO @ Sun, 21 Jun 2020 22:03:47: 4000000 INFO @ Sun, 21 Jun 2020 22:03:49: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:53: 5000000 INFO @ Sun, 21 Jun 2020 22:03:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:54: 11000000 INFO @ Sun, 21 Jun 2020 22:03:58: 6000000 INFO @ Sun, 21 Jun 2020 22:03:59: 1000000 INFO @ Sun, 21 Jun 2020 22:04:00: 12000000 INFO @ Sun, 21 Jun 2020 22:04:04: 7000000 INFO @ Sun, 21 Jun 2020 22:04:04: 2000000 INFO @ Sun, 21 Jun 2020 22:04:06: 13000000 INFO @ Sun, 21 Jun 2020 22:04:09: 8000000 INFO @ Sun, 21 Jun 2020 22:04:09: 3000000 INFO @ Sun, 21 Jun 2020 22:04:11: 14000000 INFO @ Sun, 21 Jun 2020 22:04:14: 4000000 INFO @ Sun, 21 Jun 2020 22:04:14: 9000000 INFO @ Sun, 21 Jun 2020 22:04:17: 15000000 INFO @ Sun, 21 Jun 2020 22:04:19: 5000000 INFO @ Sun, 21 Jun 2020 22:04:20: 10000000 INFO @ Sun, 21 Jun 2020 22:04:22: 16000000 INFO @ Sun, 21 Jun 2020 22:04:24: 6000000 INFO @ Sun, 21 Jun 2020 22:04:26: 11000000 INFO @ Sun, 21 Jun 2020 22:04:28: 17000000 INFO @ Sun, 21 Jun 2020 22:04:29: 7000000 INFO @ Sun, 21 Jun 2020 22:04:31: 12000000 INFO @ Sun, 21 Jun 2020 22:04:33: 18000000 INFO @ Sun, 21 Jun 2020 22:04:34: 8000000 INFO @ Sun, 21 Jun 2020 22:04:37: 13000000 INFO @ Sun, 21 Jun 2020 22:04:39: 19000000 INFO @ Sun, 21 Jun 2020 22:04:39: 9000000 INFO @ Sun, 21 Jun 2020 22:04:42: 14000000 INFO @ Sun, 21 Jun 2020 22:04:44: 10000000 INFO @ Sun, 21 Jun 2020 22:04:45: 20000000 INFO @ Sun, 21 Jun 2020 22:04:48: 15000000 INFO @ Sun, 21 Jun 2020 22:04:49: 11000000 INFO @ Sun, 21 Jun 2020 22:04:51: 21000000 INFO @ Sun, 21 Jun 2020 22:04:53: 16000000 INFO @ Sun, 21 Jun 2020 22:04:54: 12000000 INFO @ Sun, 21 Jun 2020 22:04:56: 22000000 INFO @ Sun, 21 Jun 2020 22:04:59: 17000000 INFO @ Sun, 21 Jun 2020 22:04:59: 13000000 INFO @ Sun, 21 Jun 2020 22:05:03: 23000000 INFO @ Sun, 21 Jun 2020 22:05:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:05:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:05:03: #1 total tags in treatment: 23103383 INFO @ Sun, 21 Jun 2020 22:05:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:05:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:05:04: #1 tags after filtering in treatment: 23103320 INFO @ Sun, 21 Jun 2020 22:05:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:05:04: #1 finished! INFO @ Sun, 21 Jun 2020 22:05:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:05:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:05:04: 18000000 INFO @ Sun, 21 Jun 2020 22:05:04: 14000000 INFO @ Sun, 21 Jun 2020 22:05:05: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 22:05:05: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:05:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:05:09: 15000000 INFO @ Sun, 21 Jun 2020 22:05:10: 19000000 INFO @ Sun, 21 Jun 2020 22:05:14: 16000000 INFO @ Sun, 21 Jun 2020 22:05:16: 20000000 INFO @ Sun, 21 Jun 2020 22:05:19: 17000000 INFO @ Sun, 21 Jun 2020 22:05:21: 21000000 INFO @ Sun, 21 Jun 2020 22:05:24: 18000000 INFO @ Sun, 21 Jun 2020 22:05:27: 22000000 INFO @ Sun, 21 Jun 2020 22:05:29: 19000000 INFO @ Sun, 21 Jun 2020 22:05:32: 23000000 INFO @ Sun, 21 Jun 2020 22:05:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:05:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:05:33: #1 total tags in treatment: 23103383 INFO @ Sun, 21 Jun 2020 22:05:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:05:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:05:34: #1 tags after filtering in treatment: 23103320 INFO @ Sun, 21 Jun 2020 22:05:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:05:34: #1 finished! INFO @ Sun, 21 Jun 2020 22:05:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:05:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:05:34: 20000000 INFO @ Sun, 21 Jun 2020 22:05:35: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 22:05:35: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:05:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:05:39: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:05:44: 22000000 INFO @ Sun, 21 Jun 2020 22:05:49: 23000000 INFO @ Sun, 21 Jun 2020 22:05:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:05:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:05:49: #1 total tags in treatment: 23103383 INFO @ Sun, 21 Jun 2020 22:05:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:05:50: #1 tags after filtering in treatment: 23103320 INFO @ Sun, 21 Jun 2020 22:05:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:05:50: #1 finished! INFO @ Sun, 21 Jun 2020 22:05:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:05:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:05:51: #2 number of paired peaks: 30 WARNING @ Sun, 21 Jun 2020 22:05:51: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:05:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535383/SRX5535383.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。