Job ID = 6458810 SRX = SRX5535382 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:42:37 prefetch.2.10.7: 1) Downloading 'SRR8743310'... 2020-06-21T12:42:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:47:23 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:47:23 prefetch.2.10.7: 1) 'SRR8743310' was downloaded successfully Read 31371192 spots for SRR8743310/SRR8743310.sra Written 31371192 spots for SRR8743310/SRR8743310.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:53 31371192 reads; of these: 31371192 (100.00%) were unpaired; of these: 896073 (2.86%) aligned 0 times 20098346 (64.07%) aligned exactly 1 time 10376773 (33.08%) aligned >1 times 97.14% overall alignment rate Time searching: 00:08:53 Overall time: 00:08:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10452267 / 30475119 = 0.3430 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:23: 1000000 INFO @ Sun, 21 Jun 2020 22:03:28: 2000000 INFO @ Sun, 21 Jun 2020 22:03:32: 3000000 INFO @ Sun, 21 Jun 2020 22:03:37: 4000000 INFO @ Sun, 21 Jun 2020 22:03:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:46: 6000000 INFO @ Sun, 21 Jun 2020 22:03:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:51: 7000000 INFO @ Sun, 21 Jun 2020 22:03:53: 1000000 INFO @ Sun, 21 Jun 2020 22:03:56: 8000000 INFO @ Sun, 21 Jun 2020 22:03:58: 2000000 INFO @ Sun, 21 Jun 2020 22:04:01: 9000000 INFO @ Sun, 21 Jun 2020 22:04:03: 3000000 INFO @ Sun, 21 Jun 2020 22:04:06: 10000000 INFO @ Sun, 21 Jun 2020 22:04:08: 4000000 INFO @ Sun, 21 Jun 2020 22:04:11: 11000000 INFO @ Sun, 21 Jun 2020 22:04:13: 5000000 INFO @ Sun, 21 Jun 2020 22:04:16: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:18: 6000000 INFO @ Sun, 21 Jun 2020 22:04:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:04:21: 13000000 INFO @ Sun, 21 Jun 2020 22:04:23: 7000000 INFO @ Sun, 21 Jun 2020 22:04:24: 1000000 INFO @ Sun, 21 Jun 2020 22:04:26: 14000000 INFO @ Sun, 21 Jun 2020 22:04:28: 8000000 INFO @ Sun, 21 Jun 2020 22:04:29: 2000000 INFO @ Sun, 21 Jun 2020 22:04:31: 15000000 INFO @ Sun, 21 Jun 2020 22:04:34: 9000000 INFO @ Sun, 21 Jun 2020 22:04:35: 3000000 INFO @ Sun, 21 Jun 2020 22:04:36: 16000000 INFO @ Sun, 21 Jun 2020 22:04:39: 10000000 INFO @ Sun, 21 Jun 2020 22:04:40: 4000000 INFO @ Sun, 21 Jun 2020 22:04:42: 17000000 INFO @ Sun, 21 Jun 2020 22:04:44: 11000000 INFO @ Sun, 21 Jun 2020 22:04:46: 5000000 INFO @ Sun, 21 Jun 2020 22:04:47: 18000000 INFO @ Sun, 21 Jun 2020 22:04:49: 12000000 INFO @ Sun, 21 Jun 2020 22:04:52: 6000000 INFO @ Sun, 21 Jun 2020 22:04:52: 19000000 INFO @ Sun, 21 Jun 2020 22:04:54: 13000000 INFO @ Sun, 21 Jun 2020 22:04:57: 7000000 INFO @ Sun, 21 Jun 2020 22:04:57: 20000000 INFO @ Sun, 21 Jun 2020 22:04:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:04:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:04:57: #1 total tags in treatment: 20022852 INFO @ Sun, 21 Jun 2020 22:04:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:58: #1 tags after filtering in treatment: 20022793 INFO @ Sun, 21 Jun 2020 22:04:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:58: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:59: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 22:04:59: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:05:00: 14000000 INFO @ Sun, 21 Jun 2020 22:05:02: 8000000 INFO @ Sun, 21 Jun 2020 22:05:05: 15000000 INFO @ Sun, 21 Jun 2020 22:05:07: 9000000 INFO @ Sun, 21 Jun 2020 22:05:10: 16000000 INFO @ Sun, 21 Jun 2020 22:05:12: 10000000 INFO @ Sun, 21 Jun 2020 22:05:15: 17000000 INFO @ Sun, 21 Jun 2020 22:05:17: 11000000 INFO @ Sun, 21 Jun 2020 22:05:20: 18000000 INFO @ Sun, 21 Jun 2020 22:05:22: 12000000 INFO @ Sun, 21 Jun 2020 22:05:25: 19000000 INFO @ Sun, 21 Jun 2020 22:05:28: 13000000 INFO @ Sun, 21 Jun 2020 22:05:30: 20000000 INFO @ Sun, 21 Jun 2020 22:05:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:05:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:05:30: #1 total tags in treatment: 20022852 INFO @ Sun, 21 Jun 2020 22:05:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:05:31: #1 tags after filtering in treatment: 20022793 INFO @ Sun, 21 Jun 2020 22:05:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:05:31: #1 finished! INFO @ Sun, 21 Jun 2020 22:05:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:05:32: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 22:05:32: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:05:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:05:32: 14000000 INFO @ Sun, 21 Jun 2020 22:05:37: 15000000 INFO @ Sun, 21 Jun 2020 22:05:42: 16000000 INFO @ Sun, 21 Jun 2020 22:05:48: 17000000 INFO @ Sun, 21 Jun 2020 22:05:53: 18000000 INFO @ Sun, 21 Jun 2020 22:05:58: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:06:03: 20000000 INFO @ Sun, 21 Jun 2020 22:06:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:06:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:06:03: #1 total tags in treatment: 20022852 INFO @ Sun, 21 Jun 2020 22:06:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:06:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:06:04: #1 tags after filtering in treatment: 20022793 INFO @ Sun, 21 Jun 2020 22:06:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:06:04: #1 finished! INFO @ Sun, 21 Jun 2020 22:06:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:06:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:06:05: #2 number of paired peaks: 91 WARNING @ Sun, 21 Jun 2020 22:06:05: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:06:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535382/SRX5535382.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。