Job ID = 6458806 SRX = SRX5535374 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:55:37 prefetch.2.10.7: 1) Downloading 'SRR8743302'... 2020-06-21T12:55:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T13:03:07 prefetch.2.10.7: HTTPS download succeed 2020-06-21T13:03:07 prefetch.2.10.7: 1) 'SRR8743302' was downloaded successfully Read 33501445 spots for SRR8743302/SRR8743302.sra Written 33501445 spots for SRR8743302/SRR8743302.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:49 33501445 reads; of these: 33501445 (100.00%) were unpaired; of these: 943604 (2.82%) aligned 0 times 22462750 (67.05%) aligned exactly 1 time 10095091 (30.13%) aligned >1 times 97.18% overall alignment rate Time searching: 00:09:49 Overall time: 00:09:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10772728 / 32557841 = 0.3309 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:20:34: 1000000 INFO @ Sun, 21 Jun 2020 22:20:38: 2000000 INFO @ Sun, 21 Jun 2020 22:20:43: 3000000 INFO @ Sun, 21 Jun 2020 22:20:47: 4000000 INFO @ Sun, 21 Jun 2020 22:20:52: 5000000 INFO @ Sun, 21 Jun 2020 22:20:56: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:20:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:20:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:20:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:21:01: 7000000 INFO @ Sun, 21 Jun 2020 22:21:04: 1000000 INFO @ Sun, 21 Jun 2020 22:21:05: 8000000 INFO @ Sun, 21 Jun 2020 22:21:09: 2000000 INFO @ Sun, 21 Jun 2020 22:21:10: 9000000 INFO @ Sun, 21 Jun 2020 22:21:13: 3000000 INFO @ Sun, 21 Jun 2020 22:21:14: 10000000 INFO @ Sun, 21 Jun 2020 22:21:18: 4000000 INFO @ Sun, 21 Jun 2020 22:21:19: 11000000 INFO @ Sun, 21 Jun 2020 22:21:23: 5000000 INFO @ Sun, 21 Jun 2020 22:21:23: 12000000 INFO @ Sun, 21 Jun 2020 22:21:27: 6000000 INFO @ Sun, 21 Jun 2020 22:21:28: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:21:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:21:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:21:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:21:32: 7000000 INFO @ Sun, 21 Jun 2020 22:21:32: 14000000 INFO @ Sun, 21 Jun 2020 22:21:34: 1000000 INFO @ Sun, 21 Jun 2020 22:21:36: 15000000 INFO @ Sun, 21 Jun 2020 22:21:37: 8000000 INFO @ Sun, 21 Jun 2020 22:21:39: 2000000 INFO @ Sun, 21 Jun 2020 22:21:41: 16000000 INFO @ Sun, 21 Jun 2020 22:21:41: 9000000 INFO @ Sun, 21 Jun 2020 22:21:43: 3000000 INFO @ Sun, 21 Jun 2020 22:21:45: 17000000 INFO @ Sun, 21 Jun 2020 22:21:46: 10000000 INFO @ Sun, 21 Jun 2020 22:21:48: 4000000 INFO @ Sun, 21 Jun 2020 22:21:50: 18000000 INFO @ Sun, 21 Jun 2020 22:21:50: 11000000 INFO @ Sun, 21 Jun 2020 22:21:52: 5000000 INFO @ Sun, 21 Jun 2020 22:21:55: 19000000 INFO @ Sun, 21 Jun 2020 22:21:55: 12000000 INFO @ Sun, 21 Jun 2020 22:21:57: 6000000 INFO @ Sun, 21 Jun 2020 22:21:59: 20000000 INFO @ Sun, 21 Jun 2020 22:22:00: 13000000 INFO @ Sun, 21 Jun 2020 22:22:01: 7000000 INFO @ Sun, 21 Jun 2020 22:22:04: 21000000 INFO @ Sun, 21 Jun 2020 22:22:04: 14000000 INFO @ Sun, 21 Jun 2020 22:22:06: 8000000 INFO @ Sun, 21 Jun 2020 22:22:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:22:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:22:08: #1 total tags in treatment: 21785113 INFO @ Sun, 21 Jun 2020 22:22:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:08: #1 tags after filtering in treatment: 21785063 INFO @ Sun, 21 Jun 2020 22:22:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:08: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:09: 15000000 INFO @ Sun, 21 Jun 2020 22:22:10: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 22:22:10: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:22:11: 9000000 INFO @ Sun, 21 Jun 2020 22:22:13: 16000000 INFO @ Sun, 21 Jun 2020 22:22:15: 10000000 INFO @ Sun, 21 Jun 2020 22:22:18: 17000000 INFO @ Sun, 21 Jun 2020 22:22:20: 11000000 INFO @ Sun, 21 Jun 2020 22:22:23: 18000000 INFO @ Sun, 21 Jun 2020 22:22:25: 12000000 INFO @ Sun, 21 Jun 2020 22:22:28: 19000000 INFO @ Sun, 21 Jun 2020 22:22:29: 13000000 INFO @ Sun, 21 Jun 2020 22:22:33: 20000000 INFO @ Sun, 21 Jun 2020 22:22:34: 14000000 INFO @ Sun, 21 Jun 2020 22:22:37: 21000000 INFO @ Sun, 21 Jun 2020 22:22:38: 15000000 INFO @ Sun, 21 Jun 2020 22:22:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:22:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:22:41: #1 total tags in treatment: 21785113 INFO @ Sun, 21 Jun 2020 22:22:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:22:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:22:42: #1 tags after filtering in treatment: 21785063 INFO @ Sun, 21 Jun 2020 22:22:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:22:42: #1 finished! INFO @ Sun, 21 Jun 2020 22:22:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:22:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:22:43: 16000000 INFO @ Sun, 21 Jun 2020 22:22:43: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 22:22:43: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:22:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:22:47: 17000000 INFO @ Sun, 21 Jun 2020 22:22:52: 18000000 INFO @ Sun, 21 Jun 2020 22:22:57: 19000000 INFO @ Sun, 21 Jun 2020 22:23:02: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:23:06: 21000000 INFO @ Sun, 21 Jun 2020 22:23:11: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:23:11: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:23:11: #1 total tags in treatment: 21785113 INFO @ Sun, 21 Jun 2020 22:23:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:23:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:23:11: #1 tags after filtering in treatment: 21785063 INFO @ Sun, 21 Jun 2020 22:23:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:23:11: #1 finished! INFO @ Sun, 21 Jun 2020 22:23:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:23:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:23:12: #2 number of paired peaks: 57 WARNING @ Sun, 21 Jun 2020 22:23:12: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:23:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535374/SRX5535374.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。