Job ID = 6458805 SRX = SRX5535373 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:46:40 prefetch.2.10.7: 1) Downloading 'SRR8743301'... 2020-06-21T12:46:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:50:29 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:50:29 prefetch.2.10.7: 1) 'SRR8743301' was downloaded successfully 2020-06-21T12:50:29 prefetch.2.10.7: 'SRR8743301' has 0 unresolved dependencies Read 34852432 spots for SRR8743301/SRR8743301.sra Written 34852432 spots for SRR8743301/SRR8743301.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:58 34852432 reads; of these: 34852432 (100.00%) were unpaired; of these: 860010 (2.47%) aligned 0 times 24209036 (69.46%) aligned exactly 1 time 9783386 (28.07%) aligned >1 times 97.53% overall alignment rate Time searching: 00:09:58 Overall time: 00:09:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11291562 / 33992422 = 0.3322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:12: 1000000 INFO @ Sun, 21 Jun 2020 22:08:17: 2000000 INFO @ Sun, 21 Jun 2020 22:08:22: 3000000 INFO @ Sun, 21 Jun 2020 22:08:27: 4000000 INFO @ Sun, 21 Jun 2020 22:08:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:08:36: 6000000 INFO @ Sun, 21 Jun 2020 22:08:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:08:36: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:08:36: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:08:41: 1000000 INFO @ Sun, 21 Jun 2020 22:08:41: 7000000 INFO @ Sun, 21 Jun 2020 22:08:46: 2000000 INFO @ Sun, 21 Jun 2020 22:08:46: 8000000 INFO @ Sun, 21 Jun 2020 22:08:50: 3000000 INFO @ Sun, 21 Jun 2020 22:08:51: 9000000 INFO @ Sun, 21 Jun 2020 22:08:55: 4000000 INFO @ Sun, 21 Jun 2020 22:08:56: 10000000 INFO @ Sun, 21 Jun 2020 22:09:00: 5000000 INFO @ Sun, 21 Jun 2020 22:09:01: 11000000 INFO @ Sun, 21 Jun 2020 22:09:04: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:09:06: 12000000 INFO @ Sun, 21 Jun 2020 22:09:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:09:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:09:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:09:09: 7000000 INFO @ Sun, 21 Jun 2020 22:09:11: 13000000 INFO @ Sun, 21 Jun 2020 22:09:12: 1000000 INFO @ Sun, 21 Jun 2020 22:09:14: 8000000 INFO @ Sun, 21 Jun 2020 22:09:16: 14000000 INFO @ Sun, 21 Jun 2020 22:09:17: 2000000 INFO @ Sun, 21 Jun 2020 22:09:19: 9000000 INFO @ Sun, 21 Jun 2020 22:09:21: 15000000 INFO @ Sun, 21 Jun 2020 22:09:22: 3000000 INFO @ Sun, 21 Jun 2020 22:09:23: 10000000 INFO @ Sun, 21 Jun 2020 22:09:26: 16000000 INFO @ Sun, 21 Jun 2020 22:09:26: 4000000 INFO @ Sun, 21 Jun 2020 22:09:28: 11000000 INFO @ Sun, 21 Jun 2020 22:09:31: 17000000 INFO @ Sun, 21 Jun 2020 22:09:31: 5000000 INFO @ Sun, 21 Jun 2020 22:09:33: 12000000 INFO @ Sun, 21 Jun 2020 22:09:36: 18000000 INFO @ Sun, 21 Jun 2020 22:09:36: 6000000 INFO @ Sun, 21 Jun 2020 22:09:38: 13000000 INFO @ Sun, 21 Jun 2020 22:09:41: 7000000 INFO @ Sun, 21 Jun 2020 22:09:42: 19000000 INFO @ Sun, 21 Jun 2020 22:09:42: 14000000 INFO @ Sun, 21 Jun 2020 22:09:46: 8000000 INFO @ Sun, 21 Jun 2020 22:09:47: 20000000 INFO @ Sun, 21 Jun 2020 22:09:47: 15000000 INFO @ Sun, 21 Jun 2020 22:09:50: 9000000 INFO @ Sun, 21 Jun 2020 22:09:52: 21000000 INFO @ Sun, 21 Jun 2020 22:09:52: 16000000 INFO @ Sun, 21 Jun 2020 22:09:55: 10000000 INFO @ Sun, 21 Jun 2020 22:09:57: 17000000 INFO @ Sun, 21 Jun 2020 22:09:57: 22000000 INFO @ Sun, 21 Jun 2020 22:10:00: 11000000 INFO @ Sun, 21 Jun 2020 22:10:01: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:10:01: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:10:01: #1 total tags in treatment: 22700860 INFO @ Sun, 21 Jun 2020 22:10:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:10:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:10:01: #1 tags after filtering in treatment: 22700822 INFO @ Sun, 21 Jun 2020 22:10:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:10:01: #1 finished! INFO @ Sun, 21 Jun 2020 22:10:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:10:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:10:02: 18000000 INFO @ Sun, 21 Jun 2020 22:10:03: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:10:03: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:10:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:10:05: 12000000 INFO @ Sun, 21 Jun 2020 22:10:07: 19000000 INFO @ Sun, 21 Jun 2020 22:10:09: 13000000 INFO @ Sun, 21 Jun 2020 22:10:11: 20000000 INFO @ Sun, 21 Jun 2020 22:10:14: 14000000 INFO @ Sun, 21 Jun 2020 22:10:16: 21000000 INFO @ Sun, 21 Jun 2020 22:10:19: 15000000 INFO @ Sun, 21 Jun 2020 22:10:21: 22000000 INFO @ Sun, 21 Jun 2020 22:10:24: 16000000 INFO @ Sun, 21 Jun 2020 22:10:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:10:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:10:25: #1 total tags in treatment: 22700860 INFO @ Sun, 21 Jun 2020 22:10:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:10:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:10:26: #1 tags after filtering in treatment: 22700822 INFO @ Sun, 21 Jun 2020 22:10:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:10:26: #1 finished! INFO @ Sun, 21 Jun 2020 22:10:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:10:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:10:27: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:10:27: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:10:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:10:28: 17000000 INFO @ Sun, 21 Jun 2020 22:10:33: 18000000 INFO @ Sun, 21 Jun 2020 22:10:38: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:10:42: 20000000 INFO @ Sun, 21 Jun 2020 22:10:47: 21000000 INFO @ Sun, 21 Jun 2020 22:10:52: 22000000 INFO @ Sun, 21 Jun 2020 22:10:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:10:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:10:55: #1 total tags in treatment: 22700860 INFO @ Sun, 21 Jun 2020 22:10:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:10:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:10:56: #1 tags after filtering in treatment: 22700822 INFO @ Sun, 21 Jun 2020 22:10:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:10:56: #1 finished! INFO @ Sun, 21 Jun 2020 22:10:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:10:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:10:57: #2 number of paired peaks: 31 WARNING @ Sun, 21 Jun 2020 22:10:57: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:10:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535373/SRX5535373.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。