Job ID = 6458803 SRX = SRX5535371 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:42:40 prefetch.2.10.7: 1) Downloading 'SRR8743299'... 2020-06-21T12:42:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:50:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:50:14 prefetch.2.10.7: 1) 'SRR8743299' was downloaded successfully Read 37113815 spots for SRR8743299/SRR8743299.sra Written 37113815 spots for SRR8743299/SRR8743299.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:26 37113815 reads; of these: 37113815 (100.00%) were unpaired; of these: 918282 (2.47%) aligned 0 times 25487087 (68.67%) aligned exactly 1 time 10708446 (28.85%) aligned >1 times 97.53% overall alignment rate Time searching: 00:10:26 Overall time: 00:10:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12087266 / 36195533 = 0.3339 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:09:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:09:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:09:36: 1000000 INFO @ Sun, 21 Jun 2020 22:09:42: 2000000 INFO @ Sun, 21 Jun 2020 22:09:48: 3000000 INFO @ Sun, 21 Jun 2020 22:09:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:10:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:10:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:10:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:10:01: 5000000 INFO @ Sun, 21 Jun 2020 22:10:08: 6000000 INFO @ Sun, 21 Jun 2020 22:10:08: 1000000 INFO @ Sun, 21 Jun 2020 22:10:16: 7000000 INFO @ Sun, 21 Jun 2020 22:10:17: 2000000 INFO @ Sun, 21 Jun 2020 22:10:23: 8000000 INFO @ Sun, 21 Jun 2020 22:10:25: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:10:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:10:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:10:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:10:31: 9000000 INFO @ Sun, 21 Jun 2020 22:10:33: 4000000 INFO @ Sun, 21 Jun 2020 22:10:37: 1000000 INFO @ Sun, 21 Jun 2020 22:10:38: 10000000 INFO @ Sun, 21 Jun 2020 22:10:41: 5000000 INFO @ Sun, 21 Jun 2020 22:10:45: 2000000 INFO @ Sun, 21 Jun 2020 22:10:46: 11000000 INFO @ Sun, 21 Jun 2020 22:10:50: 6000000 INFO @ Sun, 21 Jun 2020 22:10:52: 3000000 INFO @ Sun, 21 Jun 2020 22:10:53: 12000000 INFO @ Sun, 21 Jun 2020 22:10:58: 7000000 INFO @ Sun, 21 Jun 2020 22:11:00: 4000000 INFO @ Sun, 21 Jun 2020 22:11:01: 13000000 INFO @ Sun, 21 Jun 2020 22:11:06: 8000000 INFO @ Sun, 21 Jun 2020 22:11:07: 5000000 INFO @ Sun, 21 Jun 2020 22:11:08: 14000000 INFO @ Sun, 21 Jun 2020 22:11:15: 9000000 INFO @ Sun, 21 Jun 2020 22:11:15: 6000000 INFO @ Sun, 21 Jun 2020 22:11:16: 15000000 INFO @ Sun, 21 Jun 2020 22:11:22: 7000000 INFO @ Sun, 21 Jun 2020 22:11:23: 16000000 INFO @ Sun, 21 Jun 2020 22:11:23: 10000000 INFO @ Sun, 21 Jun 2020 22:11:30: 8000000 INFO @ Sun, 21 Jun 2020 22:11:30: 17000000 INFO @ Sun, 21 Jun 2020 22:11:31: 11000000 INFO @ Sun, 21 Jun 2020 22:11:37: 9000000 INFO @ Sun, 21 Jun 2020 22:11:38: 18000000 INFO @ Sun, 21 Jun 2020 22:11:40: 12000000 INFO @ Sun, 21 Jun 2020 22:11:45: 10000000 INFO @ Sun, 21 Jun 2020 22:11:45: 19000000 INFO @ Sun, 21 Jun 2020 22:11:48: 13000000 INFO @ Sun, 21 Jun 2020 22:11:52: 11000000 INFO @ Sun, 21 Jun 2020 22:11:53: 20000000 INFO @ Sun, 21 Jun 2020 22:11:57: 14000000 INFO @ Sun, 21 Jun 2020 22:11:59: 12000000 INFO @ Sun, 21 Jun 2020 22:12:01: 21000000 INFO @ Sun, 21 Jun 2020 22:12:06: 15000000 INFO @ Sun, 21 Jun 2020 22:12:07: 13000000 INFO @ Sun, 21 Jun 2020 22:12:08: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:12:14: 16000000 INFO @ Sun, 21 Jun 2020 22:12:15: 14000000 INFO @ Sun, 21 Jun 2020 22:12:16: 23000000 INFO @ Sun, 21 Jun 2020 22:12:22: 15000000 INFO @ Sun, 21 Jun 2020 22:12:23: 17000000 INFO @ Sun, 21 Jun 2020 22:12:23: 24000000 INFO @ Sun, 21 Jun 2020 22:12:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:12:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:12:24: #1 total tags in treatment: 24108267 INFO @ Sun, 21 Jun 2020 22:12:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:12:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:12:25: #1 tags after filtering in treatment: 24108228 INFO @ Sun, 21 Jun 2020 22:12:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:12:25: #1 finished! INFO @ Sun, 21 Jun 2020 22:12:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:12:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:12:27: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 22:12:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:12:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:12:30: 16000000 INFO @ Sun, 21 Jun 2020 22:12:32: 18000000 INFO @ Sun, 21 Jun 2020 22:12:37: 17000000 INFO @ Sun, 21 Jun 2020 22:12:40: 19000000 INFO @ Sun, 21 Jun 2020 22:12:44: 18000000 INFO @ Sun, 21 Jun 2020 22:12:49: 20000000 INFO @ Sun, 21 Jun 2020 22:12:52: 19000000 INFO @ Sun, 21 Jun 2020 22:12:58: 21000000 INFO @ Sun, 21 Jun 2020 22:13:00: 20000000 INFO @ Sun, 21 Jun 2020 22:13:06: 22000000 INFO @ Sun, 21 Jun 2020 22:13:07: 21000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:13:15: 22000000 INFO @ Sun, 21 Jun 2020 22:13:15: 23000000 INFO @ Sun, 21 Jun 2020 22:13:22: 23000000 INFO @ Sun, 21 Jun 2020 22:13:24: 24000000 INFO @ Sun, 21 Jun 2020 22:13:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:13:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:13:25: #1 total tags in treatment: 24108267 INFO @ Sun, 21 Jun 2020 22:13:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:25: #1 tags after filtering in treatment: 24108228 INFO @ Sun, 21 Jun 2020 22:13:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:25: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:27: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 22:13:27: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:13:29: 24000000 INFO @ Sun, 21 Jun 2020 22:13:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:13:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:13:30: #1 total tags in treatment: 24108267 INFO @ Sun, 21 Jun 2020 22:13:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:30: #1 tags after filtering in treatment: 24108228 INFO @ Sun, 21 Jun 2020 22:13:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:30: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:32: #2 number of paired peaks: 29 WARNING @ Sun, 21 Jun 2020 22:13:32: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5535371/SRX5535371.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling