Job ID = 6458708 SRX = SRX5416209 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:33:52 prefetch.2.10.7: 1) Downloading 'SRR8617259'... 2020-06-21T12:33:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:41:43 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:41:43 prefetch.2.10.7: 1) 'SRR8617259' was downloaded successfully Read 49791609 spots for SRR8617259/SRR8617259.sra Written 49791609 spots for SRR8617259/SRR8617259.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:35 49791609 reads; of these: 49791609 (100.00%) were unpaired; of these: 1754187 (3.52%) aligned 0 times 37799909 (75.92%) aligned exactly 1 time 10237513 (20.56%) aligned >1 times 96.48% overall alignment rate Time searching: 00:12:35 Overall time: 00:12:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8328246 / 48037422 = 0.1734 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:09:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:09:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:09:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:09:21: 1000000 INFO @ Sun, 21 Jun 2020 22:09:27: 2000000 INFO @ Sun, 21 Jun 2020 22:09:33: 3000000 INFO @ Sun, 21 Jun 2020 22:09:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:09:45: 5000000 INFO @ Sun, 21 Jun 2020 22:09:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:09:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:09:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:09:51: 6000000 INFO @ Sun, 21 Jun 2020 22:09:51: 1000000 INFO @ Sun, 21 Jun 2020 22:09:57: 2000000 INFO @ Sun, 21 Jun 2020 22:09:57: 7000000 INFO @ Sun, 21 Jun 2020 22:10:03: 3000000 INFO @ Sun, 21 Jun 2020 22:10:04: 8000000 INFO @ Sun, 21 Jun 2020 22:10:09: 4000000 INFO @ Sun, 21 Jun 2020 22:10:10: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:10:15: 5000000 INFO @ Sun, 21 Jun 2020 22:10:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:10:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:10:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:10:16: 10000000 INFO @ Sun, 21 Jun 2020 22:10:21: 6000000 INFO @ Sun, 21 Jun 2020 22:10:21: 1000000 INFO @ Sun, 21 Jun 2020 22:10:23: 11000000 INFO @ Sun, 21 Jun 2020 22:10:26: 7000000 INFO @ Sun, 21 Jun 2020 22:10:27: 2000000 INFO @ Sun, 21 Jun 2020 22:10:30: 12000000 INFO @ Sun, 21 Jun 2020 22:10:32: 3000000 INFO @ Sun, 21 Jun 2020 22:10:32: 8000000 INFO @ Sun, 21 Jun 2020 22:10:36: 13000000 INFO @ Sun, 21 Jun 2020 22:10:38: 4000000 INFO @ Sun, 21 Jun 2020 22:10:38: 9000000 INFO @ Sun, 21 Jun 2020 22:10:43: 14000000 INFO @ Sun, 21 Jun 2020 22:10:44: 5000000 INFO @ Sun, 21 Jun 2020 22:10:44: 10000000 INFO @ Sun, 21 Jun 2020 22:10:49: 6000000 INFO @ Sun, 21 Jun 2020 22:10:49: 15000000 INFO @ Sun, 21 Jun 2020 22:10:50: 11000000 INFO @ Sun, 21 Jun 2020 22:10:55: 7000000 INFO @ Sun, 21 Jun 2020 22:10:56: 12000000 INFO @ Sun, 21 Jun 2020 22:10:56: 16000000 INFO @ Sun, 21 Jun 2020 22:11:00: 8000000 INFO @ Sun, 21 Jun 2020 22:11:02: 13000000 INFO @ Sun, 21 Jun 2020 22:11:03: 17000000 INFO @ Sun, 21 Jun 2020 22:11:06: 9000000 INFO @ Sun, 21 Jun 2020 22:11:08: 14000000 INFO @ Sun, 21 Jun 2020 22:11:09: 18000000 INFO @ Sun, 21 Jun 2020 22:11:12: 10000000 INFO @ Sun, 21 Jun 2020 22:11:13: 15000000 INFO @ Sun, 21 Jun 2020 22:11:16: 19000000 INFO @ Sun, 21 Jun 2020 22:11:17: 11000000 INFO @ Sun, 21 Jun 2020 22:11:19: 16000000 INFO @ Sun, 21 Jun 2020 22:11:22: 20000000 INFO @ Sun, 21 Jun 2020 22:11:23: 12000000 INFO @ Sun, 21 Jun 2020 22:11:25: 17000000 INFO @ Sun, 21 Jun 2020 22:11:28: 21000000 INFO @ Sun, 21 Jun 2020 22:11:29: 13000000 INFO @ Sun, 21 Jun 2020 22:11:31: 18000000 INFO @ Sun, 21 Jun 2020 22:11:34: 14000000 INFO @ Sun, 21 Jun 2020 22:11:34: 22000000 INFO @ Sun, 21 Jun 2020 22:11:36: 19000000 INFO @ Sun, 21 Jun 2020 22:11:40: 15000000 INFO @ Sun, 21 Jun 2020 22:11:41: 23000000 INFO @ Sun, 21 Jun 2020 22:11:42: 20000000 INFO @ Sun, 21 Jun 2020 22:11:46: 16000000 INFO @ Sun, 21 Jun 2020 22:11:47: 24000000 INFO @ Sun, 21 Jun 2020 22:11:48: 21000000 INFO @ Sun, 21 Jun 2020 22:11:51: 17000000 INFO @ Sun, 21 Jun 2020 22:11:53: 22000000 INFO @ Sun, 21 Jun 2020 22:11:54: 25000000 INFO @ Sun, 21 Jun 2020 22:11:57: 18000000 INFO @ Sun, 21 Jun 2020 22:11:59: 23000000 INFO @ Sun, 21 Jun 2020 22:12:00: 26000000 INFO @ Sun, 21 Jun 2020 22:12:03: 19000000 INFO @ Sun, 21 Jun 2020 22:12:05: 24000000 INFO @ Sun, 21 Jun 2020 22:12:07: 27000000 INFO @ Sun, 21 Jun 2020 22:12:08: 20000000 INFO @ Sun, 21 Jun 2020 22:12:11: 25000000 INFO @ Sun, 21 Jun 2020 22:12:13: 28000000 INFO @ Sun, 21 Jun 2020 22:12:14: 21000000 INFO @ Sun, 21 Jun 2020 22:12:16: 26000000 INFO @ Sun, 21 Jun 2020 22:12:19: 22000000 INFO @ Sun, 21 Jun 2020 22:12:20: 29000000 INFO @ Sun, 21 Jun 2020 22:12:22: 27000000 INFO @ Sun, 21 Jun 2020 22:12:25: 23000000 INFO @ Sun, 21 Jun 2020 22:12:27: 30000000 INFO @ Sun, 21 Jun 2020 22:12:28: 28000000 INFO @ Sun, 21 Jun 2020 22:12:31: 24000000 INFO @ Sun, 21 Jun 2020 22:12:34: 29000000 INFO @ Sun, 21 Jun 2020 22:12:34: 31000000 INFO @ Sun, 21 Jun 2020 22:12:36: 25000000 INFO @ Sun, 21 Jun 2020 22:12:39: 30000000 INFO @ Sun, 21 Jun 2020 22:12:41: 32000000 INFO @ Sun, 21 Jun 2020 22:12:42: 26000000 INFO @ Sun, 21 Jun 2020 22:12:45: 31000000 INFO @ Sun, 21 Jun 2020 22:12:48: 27000000 INFO @ Sun, 21 Jun 2020 22:12:48: 33000000 INFO @ Sun, 21 Jun 2020 22:12:51: 32000000 INFO @ Sun, 21 Jun 2020 22:12:53: 28000000 INFO @ Sun, 21 Jun 2020 22:12:54: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:12:57: 33000000 INFO @ Sun, 21 Jun 2020 22:12:59: 29000000 INFO @ Sun, 21 Jun 2020 22:13:01: 35000000 INFO @ Sun, 21 Jun 2020 22:13:02: 34000000 INFO @ Sun, 21 Jun 2020 22:13:05: 30000000 INFO @ Sun, 21 Jun 2020 22:13:08: 36000000 INFO @ Sun, 21 Jun 2020 22:13:08: 35000000 INFO @ Sun, 21 Jun 2020 22:13:10: 31000000 INFO @ Sun, 21 Jun 2020 22:13:14: 36000000 INFO @ Sun, 21 Jun 2020 22:13:15: 37000000 INFO @ Sun, 21 Jun 2020 22:13:16: 32000000 INFO @ Sun, 21 Jun 2020 22:13:20: 37000000 INFO @ Sun, 21 Jun 2020 22:13:22: 38000000 INFO @ Sun, 21 Jun 2020 22:13:22: 33000000 INFO @ Sun, 21 Jun 2020 22:13:25: 38000000 INFO @ Sun, 21 Jun 2020 22:13:27: 34000000 INFO @ Sun, 21 Jun 2020 22:13:29: 39000000 INFO @ Sun, 21 Jun 2020 22:13:32: 39000000 INFO @ Sun, 21 Jun 2020 22:13:33: 35000000 INFO @ Sun, 21 Jun 2020 22:13:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:13:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:13:34: #1 total tags in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:34: #1 tags after filtering in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:34: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:13:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:13:36: #1 total tags in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:37: #1 tags after filtering in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:37: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:13:37: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 22:13:37: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:13:38: 36000000 INFO @ Sun, 21 Jun 2020 22:13:39: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 22:13:39: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:13:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:13:44: 37000000 INFO @ Sun, 21 Jun 2020 22:13:49: 38000000 INFO @ Sun, 21 Jun 2020 22:13:54: 39000000 INFO @ Sun, 21 Jun 2020 22:13:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:13:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:13:59: #1 total tags in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:13:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:13:59: #1 tags after filtering in treatment: 39709176 INFO @ Sun, 21 Jun 2020 22:13:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:13:59: #1 finished! INFO @ Sun, 21 Jun 2020 22:13:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:13:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:14:02: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 22:14:02: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:14:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5416209/SRX5416209.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。