Job ID = 6529924 SRX = SRX5360592 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:14 1502232 reads; of these: 1502232 (100.00%) were unpaired; of these: 527492 (35.11%) aligned 0 times 956179 (63.65%) aligned exactly 1 time 18561 (1.24%) aligned >1 times 64.89% overall alignment rate Time searching: 00:00:14 Overall time: 00:00:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 890685 / 974740 = 0.9138 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:57:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:57:25: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:57:25: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:57:26: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 02:57:26: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 02:57:26: #1 total tags in treatment: 84055 INFO @ Tue, 30 Jun 2020 02:57:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:57:26: #1 tags after filtering in treatment: 84012 INFO @ Tue, 30 Jun 2020 02:57:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:57:26: #1 finished! INFO @ Tue, 30 Jun 2020 02:57:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:57:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:57:26: #2 number of paired peaks: 6 WARNING @ Tue, 30 Jun 2020 02:57:26: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:57:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 02:57:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:57:55: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:57:55: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:57:56: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 02:57:56: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 02:57:56: #1 total tags in treatment: 84055 INFO @ Tue, 30 Jun 2020 02:57:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:57:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:57:56: #1 tags after filtering in treatment: 84012 INFO @ Tue, 30 Jun 2020 02:57:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:57:56: #1 finished! INFO @ Tue, 30 Jun 2020 02:57:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:57:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:57:56: #2 number of paired peaks: 6 WARNING @ Tue, 30 Jun 2020 02:57:56: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:57:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 02:58:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 02:58:25: #1 read tag files... INFO @ Tue, 30 Jun 2020 02:58:25: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 02:58:26: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 02:58:26: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 02:58:26: #1 total tags in treatment: 84055 INFO @ Tue, 30 Jun 2020 02:58:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 02:58:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 02:58:26: #1 tags after filtering in treatment: 84012 INFO @ Tue, 30 Jun 2020 02:58:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 02:58:26: #1 finished! INFO @ Tue, 30 Jun 2020 02:58:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 02:58:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 02:58:26: #2 number of paired peaks: 6 WARNING @ Tue, 30 Jun 2020 02:58:26: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 02:58:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5360592/SRX5360592.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling