Job ID = 6458595 SRX = SRX5343130 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:52:10 prefetch.2.10.7: 1) Downloading 'SRR8540830'... 2020-06-21T12:52:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:57:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:57:26 prefetch.2.10.7: 1) 'SRR8540830' was downloaded successfully 2020-06-21T12:57:26 prefetch.2.10.7: 'SRR8540830' has 0 unresolved dependencies Read 53659973 spots for SRR8540830/SRR8540830.sra Written 53659973 spots for SRR8540830/SRR8540830.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:33 53659973 reads; of these: 53659973 (100.00%) were unpaired; of these: 2293370 (4.27%) aligned 0 times 35409607 (65.99%) aligned exactly 1 time 15956996 (29.74%) aligned >1 times 95.73% overall alignment rate Time searching: 00:19:33 Overall time: 00:19:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10272793 / 51366603 = 0.2000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:34:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:34:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:34:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:34:22: 1000000 INFO @ Sun, 21 Jun 2020 22:34:28: 2000000 INFO @ Sun, 21 Jun 2020 22:34:34: 3000000 INFO @ Sun, 21 Jun 2020 22:34:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:34:46: 5000000 INFO @ Sun, 21 Jun 2020 22:34:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:34:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:34:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:34:53: 6000000 INFO @ Sun, 21 Jun 2020 22:34:53: 1000000 INFO @ Sun, 21 Jun 2020 22:35:00: 7000000 INFO @ Sun, 21 Jun 2020 22:35:00: 2000000 INFO @ Sun, 21 Jun 2020 22:35:07: 8000000 INFO @ Sun, 21 Jun 2020 22:35:07: 3000000 INFO @ Sun, 21 Jun 2020 22:35:14: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:35:14: 4000000 INFO @ Sun, 21 Jun 2020 22:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:35:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:35:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:35:21: 10000000 INFO @ Sun, 21 Jun 2020 22:35:21: 5000000 INFO @ Sun, 21 Jun 2020 22:35:23: 1000000 INFO @ Sun, 21 Jun 2020 22:35:28: 11000000 INFO @ Sun, 21 Jun 2020 22:35:28: 6000000 INFO @ Sun, 21 Jun 2020 22:35:30: 2000000 INFO @ Sun, 21 Jun 2020 22:35:35: 12000000 INFO @ Sun, 21 Jun 2020 22:35:35: 7000000 INFO @ Sun, 21 Jun 2020 22:35:38: 3000000 INFO @ Sun, 21 Jun 2020 22:35:42: 13000000 INFO @ Sun, 21 Jun 2020 22:35:42: 8000000 INFO @ Sun, 21 Jun 2020 22:35:45: 4000000 INFO @ Sun, 21 Jun 2020 22:35:50: 14000000 INFO @ Sun, 21 Jun 2020 22:35:50: 9000000 INFO @ Sun, 21 Jun 2020 22:35:52: 5000000 INFO @ Sun, 21 Jun 2020 22:35:57: 15000000 INFO @ Sun, 21 Jun 2020 22:35:57: 10000000 INFO @ Sun, 21 Jun 2020 22:35:59: 6000000 INFO @ Sun, 21 Jun 2020 22:36:04: 16000000 INFO @ Sun, 21 Jun 2020 22:36:04: 11000000 INFO @ Sun, 21 Jun 2020 22:36:06: 7000000 INFO @ Sun, 21 Jun 2020 22:36:11: 17000000 INFO @ Sun, 21 Jun 2020 22:36:11: 12000000 INFO @ Sun, 21 Jun 2020 22:36:14: 8000000 INFO @ Sun, 21 Jun 2020 22:36:18: 18000000 INFO @ Sun, 21 Jun 2020 22:36:18: 13000000 INFO @ Sun, 21 Jun 2020 22:36:21: 9000000 INFO @ Sun, 21 Jun 2020 22:36:25: 19000000 INFO @ Sun, 21 Jun 2020 22:36:26: 14000000 INFO @ Sun, 21 Jun 2020 22:36:29: 10000000 INFO @ Sun, 21 Jun 2020 22:36:33: 20000000 INFO @ Sun, 21 Jun 2020 22:36:34: 15000000 INFO @ Sun, 21 Jun 2020 22:36:36: 11000000 INFO @ Sun, 21 Jun 2020 22:36:41: 21000000 INFO @ Sun, 21 Jun 2020 22:36:41: 16000000 INFO @ Sun, 21 Jun 2020 22:36:44: 12000000 INFO @ Sun, 21 Jun 2020 22:36:48: 22000000 INFO @ Sun, 21 Jun 2020 22:36:49: 17000000 INFO @ Sun, 21 Jun 2020 22:36:52: 13000000 INFO @ Sun, 21 Jun 2020 22:36:56: 23000000 INFO @ Sun, 21 Jun 2020 22:36:56: 18000000 INFO @ Sun, 21 Jun 2020 22:36:59: 14000000 INFO @ Sun, 21 Jun 2020 22:37:03: 24000000 INFO @ Sun, 21 Jun 2020 22:37:04: 19000000 INFO @ Sun, 21 Jun 2020 22:37:07: 15000000 INFO @ Sun, 21 Jun 2020 22:37:10: 25000000 INFO @ Sun, 21 Jun 2020 22:37:12: 20000000 INFO @ Sun, 21 Jun 2020 22:37:14: 16000000 INFO @ Sun, 21 Jun 2020 22:37:18: 26000000 INFO @ Sun, 21 Jun 2020 22:37:19: 21000000 INFO @ Sun, 21 Jun 2020 22:37:22: 17000000 INFO @ Sun, 21 Jun 2020 22:37:25: 27000000 INFO @ Sun, 21 Jun 2020 22:37:26: 22000000 INFO @ Sun, 21 Jun 2020 22:37:29: 18000000 INFO @ Sun, 21 Jun 2020 22:37:32: 28000000 INFO @ Sun, 21 Jun 2020 22:37:34: 23000000 INFO @ Sun, 21 Jun 2020 22:37:37: 19000000 INFO @ Sun, 21 Jun 2020 22:37:40: 29000000 INFO @ Sun, 21 Jun 2020 22:37:41: 24000000 INFO @ Sun, 21 Jun 2020 22:37:44: 20000000 INFO @ Sun, 21 Jun 2020 22:37:47: 30000000 INFO @ Sun, 21 Jun 2020 22:37:49: 25000000 INFO @ Sun, 21 Jun 2020 22:37:52: 21000000 INFO @ Sun, 21 Jun 2020 22:37:54: 31000000 INFO @ Sun, 21 Jun 2020 22:37:56: 26000000 INFO @ Sun, 21 Jun 2020 22:37:59: 22000000 INFO @ Sun, 21 Jun 2020 22:38:02: 32000000 INFO @ Sun, 21 Jun 2020 22:38:03: 27000000 INFO @ Sun, 21 Jun 2020 22:38:06: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:38:09: 33000000 INFO @ Sun, 21 Jun 2020 22:38:11: 28000000 INFO @ Sun, 21 Jun 2020 22:38:14: 24000000 INFO @ Sun, 21 Jun 2020 22:38:16: 34000000 INFO @ Sun, 21 Jun 2020 22:38:18: 29000000 INFO @ Sun, 21 Jun 2020 22:38:21: 25000000 INFO @ Sun, 21 Jun 2020 22:38:24: 35000000 INFO @ Sun, 21 Jun 2020 22:38:25: 30000000 INFO @ Sun, 21 Jun 2020 22:38:28: 26000000 INFO @ Sun, 21 Jun 2020 22:38:31: 36000000 INFO @ Sun, 21 Jun 2020 22:38:33: 31000000 INFO @ Sun, 21 Jun 2020 22:38:36: 27000000 INFO @ Sun, 21 Jun 2020 22:38:39: 37000000 INFO @ Sun, 21 Jun 2020 22:38:40: 32000000 INFO @ Sun, 21 Jun 2020 22:38:43: 28000000 INFO @ Sun, 21 Jun 2020 22:38:46: 38000000 INFO @ Sun, 21 Jun 2020 22:38:47: 33000000 INFO @ Sun, 21 Jun 2020 22:38:51: 29000000 INFO @ Sun, 21 Jun 2020 22:38:53: 39000000 INFO @ Sun, 21 Jun 2020 22:38:55: 34000000 INFO @ Sun, 21 Jun 2020 22:38:58: 30000000 INFO @ Sun, 21 Jun 2020 22:39:01: 40000000 INFO @ Sun, 21 Jun 2020 22:39:02: 35000000 INFO @ Sun, 21 Jun 2020 22:39:05: 31000000 INFO @ Sun, 21 Jun 2020 22:39:08: 41000000 INFO @ Sun, 21 Jun 2020 22:39:09: #1 tag size is determined as 65 bps INFO @ Sun, 21 Jun 2020 22:39:09: #1 tag size = 65 INFO @ Sun, 21 Jun 2020 22:39:09: #1 total tags in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:39:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:39:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:39:10: 36000000 INFO @ Sun, 21 Jun 2020 22:39:10: #1 tags after filtering in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:39:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:39:10: #1 finished! INFO @ Sun, 21 Jun 2020 22:39:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:39:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:39:12: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 22:39:12: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:39:12: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 22:39:12: 32000000 cut: /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:39:17: 37000000 INFO @ Sun, 21 Jun 2020 22:39:20: 33000000 INFO @ Sun, 21 Jun 2020 22:39:24: 38000000 INFO @ Sun, 21 Jun 2020 22:39:27: 34000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:39:31: 39000000 INFO @ Sun, 21 Jun 2020 22:39:34: 35000000 INFO @ Sun, 21 Jun 2020 22:39:38: 40000000 INFO @ Sun, 21 Jun 2020 22:39:41: 36000000 INFO @ Sun, 21 Jun 2020 22:39:45: 41000000 INFO @ Sun, 21 Jun 2020 22:39:46: #1 tag size is determined as 65 bps INFO @ Sun, 21 Jun 2020 22:39:46: #1 tag size = 65 INFO @ Sun, 21 Jun 2020 22:39:46: #1 total tags in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:39:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:39:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:39:47: #1 tags after filtering in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:39:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:39:47: #1 finished! INFO @ Sun, 21 Jun 2020 22:39:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:39:48: 37000000 INFO @ Sun, 21 Jun 2020 22:39:50: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 22:39:50: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:39:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:39:54: 38000000 INFO @ Sun, 21 Jun 2020 22:40:00: 39000000 INFO @ Sun, 21 Jun 2020 22:40:07: 40000000 INFO @ Sun, 21 Jun 2020 22:40:13: 41000000 INFO @ Sun, 21 Jun 2020 22:40:13: #1 tag size is determined as 65 bps INFO @ Sun, 21 Jun 2020 22:40:13: #1 tag size = 65 INFO @ Sun, 21 Jun 2020 22:40:13: #1 total tags in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:40:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:40:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:40:14: #1 tags after filtering in treatment: 41093810 INFO @ Sun, 21 Jun 2020 22:40:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:40:14: #1 finished! INFO @ Sun, 21 Jun 2020 22:40:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:40:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:40:17: #2 number of paired peaks: 80 WARNING @ Sun, 21 Jun 2020 22:40:17: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:40:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5343130/SRX5343130.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling