Job ID = 12265539 SRX = SRX5287836 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:04 73805409 reads; of these: 73805409 (100.00%) were unpaired; of these: 3041433 (4.12%) aligned 0 times 61878798 (83.84%) aligned exactly 1 time 8885178 (12.04%) aligned >1 times 95.88% overall alignment rate Time searching: 00:18:04 Overall time: 00:18:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 38173224 / 70763976 = 0.5394 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:02:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:02:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:02:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:03:00: 1000000 INFO @ Sat, 03 Apr 2021 08:03:06: 2000000 INFO @ Sat, 03 Apr 2021 08:03:13: 3000000 INFO @ Sat, 03 Apr 2021 08:03:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:03:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:03:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:03:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:03:26: 5000000 INFO @ Sat, 03 Apr 2021 08:03:30: 1000000 INFO @ Sat, 03 Apr 2021 08:03:32: 6000000 INFO @ Sat, 03 Apr 2021 08:03:37: 2000000 INFO @ Sat, 03 Apr 2021 08:03:39: 7000000 INFO @ Sat, 03 Apr 2021 08:03:44: 3000000 INFO @ Sat, 03 Apr 2021 08:03:46: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:03:51: 4000000 INFO @ Sat, 03 Apr 2021 08:03:53: 9000000 INFO @ Sat, 03 Apr 2021 08:03:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:03:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:03:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:03:58: 5000000 INFO @ Sat, 03 Apr 2021 08:04:00: 10000000 INFO @ Sat, 03 Apr 2021 08:04:00: 1000000 INFO @ Sat, 03 Apr 2021 08:04:05: 6000000 INFO @ Sat, 03 Apr 2021 08:04:07: 11000000 INFO @ Sat, 03 Apr 2021 08:04:07: 2000000 INFO @ Sat, 03 Apr 2021 08:04:12: 7000000 INFO @ Sat, 03 Apr 2021 08:04:13: 12000000 INFO @ Sat, 03 Apr 2021 08:04:13: 3000000 INFO @ Sat, 03 Apr 2021 08:04:19: 8000000 INFO @ Sat, 03 Apr 2021 08:04:20: 13000000 INFO @ Sat, 03 Apr 2021 08:04:20: 4000000 INFO @ Sat, 03 Apr 2021 08:04:26: 9000000 INFO @ Sat, 03 Apr 2021 08:04:27: 5000000 INFO @ Sat, 03 Apr 2021 08:04:27: 14000000 INFO @ Sat, 03 Apr 2021 08:04:33: 10000000 INFO @ Sat, 03 Apr 2021 08:04:34: 6000000 INFO @ Sat, 03 Apr 2021 08:04:34: 15000000 INFO @ Sat, 03 Apr 2021 08:04:40: 11000000 INFO @ Sat, 03 Apr 2021 08:04:40: 7000000 INFO @ Sat, 03 Apr 2021 08:04:41: 16000000 INFO @ Sat, 03 Apr 2021 08:04:47: 12000000 INFO @ Sat, 03 Apr 2021 08:04:47: 8000000 INFO @ Sat, 03 Apr 2021 08:04:47: 17000000 INFO @ Sat, 03 Apr 2021 08:04:53: 13000000 INFO @ Sat, 03 Apr 2021 08:04:54: 9000000 INFO @ Sat, 03 Apr 2021 08:04:54: 18000000 INFO @ Sat, 03 Apr 2021 08:05:00: 14000000 INFO @ Sat, 03 Apr 2021 08:05:01: 10000000 INFO @ Sat, 03 Apr 2021 08:05:01: 19000000 INFO @ Sat, 03 Apr 2021 08:05:07: 11000000 INFO @ Sat, 03 Apr 2021 08:05:07: 15000000 INFO @ Sat, 03 Apr 2021 08:05:08: 20000000 INFO @ Sat, 03 Apr 2021 08:05:14: 12000000 INFO @ Sat, 03 Apr 2021 08:05:14: 16000000 INFO @ Sat, 03 Apr 2021 08:05:15: 21000000 INFO @ Sat, 03 Apr 2021 08:05:21: 13000000 INFO @ Sat, 03 Apr 2021 08:05:21: 17000000 INFO @ Sat, 03 Apr 2021 08:05:22: 22000000 INFO @ Sat, 03 Apr 2021 08:05:27: 14000000 INFO @ Sat, 03 Apr 2021 08:05:28: 23000000 INFO @ Sat, 03 Apr 2021 08:05:28: 18000000 INFO @ Sat, 03 Apr 2021 08:05:34: 15000000 INFO @ Sat, 03 Apr 2021 08:05:35: 24000000 INFO @ Sat, 03 Apr 2021 08:05:35: 19000000 INFO @ Sat, 03 Apr 2021 08:05:41: 16000000 INFO @ Sat, 03 Apr 2021 08:05:42: 25000000 INFO @ Sat, 03 Apr 2021 08:05:42: 20000000 INFO @ Sat, 03 Apr 2021 08:05:48: 17000000 INFO @ Sat, 03 Apr 2021 08:05:48: 26000000 INFO @ Sat, 03 Apr 2021 08:05:49: 21000000 INFO @ Sat, 03 Apr 2021 08:05:54: 18000000 INFO @ Sat, 03 Apr 2021 08:05:56: 22000000 INFO @ Sat, 03 Apr 2021 08:05:56: 27000000 INFO @ Sat, 03 Apr 2021 08:06:01: 19000000 INFO @ Sat, 03 Apr 2021 08:06:03: 23000000 INFO @ Sat, 03 Apr 2021 08:06:03: 28000000 INFO @ Sat, 03 Apr 2021 08:06:08: 20000000 INFO @ Sat, 03 Apr 2021 08:06:09: 24000000 INFO @ Sat, 03 Apr 2021 08:06:09: 29000000 INFO @ Sat, 03 Apr 2021 08:06:15: 21000000 INFO @ Sat, 03 Apr 2021 08:06:16: 25000000 INFO @ Sat, 03 Apr 2021 08:06:16: 30000000 INFO @ Sat, 03 Apr 2021 08:06:21: 22000000 INFO @ Sat, 03 Apr 2021 08:06:23: 26000000 INFO @ Sat, 03 Apr 2021 08:06:23: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:06:28: 23000000 INFO @ Sat, 03 Apr 2021 08:06:30: 32000000 INFO @ Sat, 03 Apr 2021 08:06:30: 27000000 INFO @ Sat, 03 Apr 2021 08:06:34: #1 tag size is determined as 64 bps INFO @ Sat, 03 Apr 2021 08:06:34: #1 tag size = 64 INFO @ Sat, 03 Apr 2021 08:06:34: #1 total tags in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:06:34: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:06:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:06:34: 24000000 INFO @ Sat, 03 Apr 2021 08:06:35: #1 tags after filtering in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:06:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:06:35: #1 finished! INFO @ Sat, 03 Apr 2021 08:06:35: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:06:37: 28000000 INFO @ Sat, 03 Apr 2021 08:06:37: #2 number of paired peaks: 30 WARNING @ Sat, 03 Apr 2021 08:06:37: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:06:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:06:41: 25000000 INFO @ Sat, 03 Apr 2021 08:06:43: 29000000 INFO @ Sat, 03 Apr 2021 08:06:47: 26000000 INFO @ Sat, 03 Apr 2021 08:06:50: 30000000 INFO @ Sat, 03 Apr 2021 08:06:54: 27000000 INFO @ Sat, 03 Apr 2021 08:06:57: 31000000 INFO @ Sat, 03 Apr 2021 08:07:01: 28000000 INFO @ Sat, 03 Apr 2021 08:07:03: 32000000 INFO @ Sat, 03 Apr 2021 08:07:08: 29000000 INFO @ Sat, 03 Apr 2021 08:07:08: #1 tag size is determined as 64 bps INFO @ Sat, 03 Apr 2021 08:07:08: #1 tag size = 64 INFO @ Sat, 03 Apr 2021 08:07:08: #1 total tags in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:07:08: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:09: #1 tags after filtering in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:07:09: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:09: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:07:11: #2 number of paired peaks: 30 WARNING @ Sat, 03 Apr 2021 08:07:11: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:07:14: 30000000 INFO @ Sat, 03 Apr 2021 08:07:20: 31000000 INFO @ Sat, 03 Apr 2021 08:07:27: 32000000 INFO @ Sat, 03 Apr 2021 08:07:31: #1 tag size is determined as 64 bps INFO @ Sat, 03 Apr 2021 08:07:31: #1 tag size = 64 INFO @ Sat, 03 Apr 2021 08:07:31: #1 total tags in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:07:31: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:32: #1 tags after filtering in treatment: 32590752 INFO @ Sat, 03 Apr 2021 08:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:07:32: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:32: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:07:34: #2 number of paired peaks: 30 WARNING @ Sat, 03 Apr 2021 08:07:34: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:07:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5287836/SRX5287836.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling