Job ID = 6458504 SRX = SRX5247954 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:37:44 prefetch.2.10.7: 1) Downloading 'SRR8440601'... 2020-06-21T12:37:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:41:05 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:41:05 prefetch.2.10.7: 1) 'SRR8440601' was downloaded successfully Read 34840378 spots for SRR8440601/SRR8440601.sra Written 34840378 spots for SRR8440601/SRR8440601.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:15 34840378 reads; of these: 34840378 (100.00%) were unpaired; of these: 2162892 (6.21%) aligned 0 times 28434842 (81.61%) aligned exactly 1 time 4242644 (12.18%) aligned >1 times 93.79% overall alignment rate Time searching: 00:08:15 Overall time: 00:08:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4482602 / 32677486 = 0.1372 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:57:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:57:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:57:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:01: 1000000 INFO @ Sun, 21 Jun 2020 21:58:06: 2000000 INFO @ Sun, 21 Jun 2020 21:58:11: 3000000 INFO @ Sun, 21 Jun 2020 21:58:16: 4000000 INFO @ Sun, 21 Jun 2020 21:58:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:25: 6000000 INFO @ Sun, 21 Jun 2020 21:58:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:30: 7000000 INFO @ Sun, 21 Jun 2020 21:58:32: 1000000 INFO @ Sun, 21 Jun 2020 21:58:35: 8000000 INFO @ Sun, 21 Jun 2020 21:58:37: 2000000 INFO @ Sun, 21 Jun 2020 21:58:40: 9000000 INFO @ Sun, 21 Jun 2020 21:58:43: 3000000 INFO @ Sun, 21 Jun 2020 21:58:44: 10000000 INFO @ Sun, 21 Jun 2020 21:58:48: 4000000 INFO @ Sun, 21 Jun 2020 21:58:49: 11000000 INFO @ Sun, 21 Jun 2020 21:58:53: 5000000 INFO @ Sun, 21 Jun 2020 21:58:54: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:58:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:58:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:58:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:58:59: 6000000 INFO @ Sun, 21 Jun 2020 21:58:59: 13000000 INFO @ Sun, 21 Jun 2020 21:59:02: 1000000 INFO @ Sun, 21 Jun 2020 21:59:04: 14000000 INFO @ Sun, 21 Jun 2020 21:59:05: 7000000 INFO @ Sun, 21 Jun 2020 21:59:07: 2000000 INFO @ Sun, 21 Jun 2020 21:59:09: 15000000 INFO @ Sun, 21 Jun 2020 21:59:10: 8000000 INFO @ Sun, 21 Jun 2020 21:59:12: 3000000 INFO @ Sun, 21 Jun 2020 21:59:14: 16000000 INFO @ Sun, 21 Jun 2020 21:59:16: 9000000 INFO @ Sun, 21 Jun 2020 21:59:16: 4000000 INFO @ Sun, 21 Jun 2020 21:59:19: 17000000 INFO @ Sun, 21 Jun 2020 21:59:21: 5000000 INFO @ Sun, 21 Jun 2020 21:59:21: 10000000 INFO @ Sun, 21 Jun 2020 21:59:24: 18000000 INFO @ Sun, 21 Jun 2020 21:59:26: 6000000 INFO @ Sun, 21 Jun 2020 21:59:27: 11000000 INFO @ Sun, 21 Jun 2020 21:59:29: 19000000 INFO @ Sun, 21 Jun 2020 21:59:31: 7000000 INFO @ Sun, 21 Jun 2020 21:59:33: 12000000 INFO @ Sun, 21 Jun 2020 21:59:34: 20000000 INFO @ Sun, 21 Jun 2020 21:59:36: 8000000 INFO @ Sun, 21 Jun 2020 21:59:38: 13000000 INFO @ Sun, 21 Jun 2020 21:59:39: 21000000 INFO @ Sun, 21 Jun 2020 21:59:40: 9000000 INFO @ Sun, 21 Jun 2020 21:59:43: 14000000 INFO @ Sun, 21 Jun 2020 21:59:44: 22000000 INFO @ Sun, 21 Jun 2020 21:59:45: 10000000 INFO @ Sun, 21 Jun 2020 21:59:49: 15000000 INFO @ Sun, 21 Jun 2020 21:59:49: 23000000 INFO @ Sun, 21 Jun 2020 21:59:50: 11000000 INFO @ Sun, 21 Jun 2020 21:59:54: 24000000 INFO @ Sun, 21 Jun 2020 21:59:54: 16000000 INFO @ Sun, 21 Jun 2020 21:59:55: 12000000 INFO @ Sun, 21 Jun 2020 21:59:59: 25000000 INFO @ Sun, 21 Jun 2020 22:00:00: 13000000 INFO @ Sun, 21 Jun 2020 22:00:00: 17000000 INFO @ Sun, 21 Jun 2020 22:00:04: 26000000 INFO @ Sun, 21 Jun 2020 22:00:04: 14000000 INFO @ Sun, 21 Jun 2020 22:00:06: 18000000 INFO @ Sun, 21 Jun 2020 22:00:09: 27000000 INFO @ Sun, 21 Jun 2020 22:00:09: 15000000 INFO @ Sun, 21 Jun 2020 22:00:11: 19000000 INFO @ Sun, 21 Jun 2020 22:00:14: 28000000 INFO @ Sun, 21 Jun 2020 22:00:14: 16000000 INFO @ Sun, 21 Jun 2020 22:00:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:00:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:00:15: #1 total tags in treatment: 28194884 INFO @ Sun, 21 Jun 2020 22:00:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:00:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:00:16: #1 tags after filtering in treatment: 28194810 INFO @ Sun, 21 Jun 2020 22:00:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:00:16: #1 finished! INFO @ Sun, 21 Jun 2020 22:00:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:00:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:00:17: 20000000 INFO @ Sun, 21 Jun 2020 22:00:17: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 22:00:17: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:00:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:00:19: 17000000 INFO @ Sun, 21 Jun 2020 22:00:22: 21000000 INFO @ Sun, 21 Jun 2020 22:00:23: 18000000 INFO @ Sun, 21 Jun 2020 22:00:28: 22000000 INFO @ Sun, 21 Jun 2020 22:00:28: 19000000 INFO @ Sun, 21 Jun 2020 22:00:33: 20000000 INFO @ Sun, 21 Jun 2020 22:00:34: 23000000 INFO @ Sun, 21 Jun 2020 22:00:38: 21000000 INFO @ Sun, 21 Jun 2020 22:00:39: 24000000 INFO @ Sun, 21 Jun 2020 22:00:43: 22000000 INFO @ Sun, 21 Jun 2020 22:00:45: 25000000 INFO @ Sun, 21 Jun 2020 22:00:48: 23000000 INFO @ Sun, 21 Jun 2020 22:00:50: 26000000 INFO @ Sun, 21 Jun 2020 22:00:53: 24000000 INFO @ Sun, 21 Jun 2020 22:00:56: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:00:58: 25000000 INFO @ Sun, 21 Jun 2020 22:01:01: 28000000 INFO @ Sun, 21 Jun 2020 22:01:03: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:01:03: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:01:03: #1 total tags in treatment: 28194884 INFO @ Sun, 21 Jun 2020 22:01:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:01:03: 26000000 INFO @ Sun, 21 Jun 2020 22:01:04: #1 tags after filtering in treatment: 28194810 INFO @ Sun, 21 Jun 2020 22:01:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:01:04: #1 finished! INFO @ Sun, 21 Jun 2020 22:01:04: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:01:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:01:05: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 22:01:05: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:01:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:01:08: 27000000 INFO @ Sun, 21 Jun 2020 22:01:13: 28000000 INFO @ Sun, 21 Jun 2020 22:01:14: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:01:14: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:01:14: #1 total tags in treatment: 28194884 INFO @ Sun, 21 Jun 2020 22:01:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:01:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:01:15: #1 tags after filtering in treatment: 28194810 INFO @ Sun, 21 Jun 2020 22:01:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:01:15: #1 finished! INFO @ Sun, 21 Jun 2020 22:01:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:01:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:01:16: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 22:01:16: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:01:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247954/SRX5247954.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。