Job ID = 6458501 SRX = SRX5247953 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:30:07 prefetch.2.10.7: 1) Downloading 'SRR8440600'... 2020-06-21T12:30:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:34:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:34:25 prefetch.2.10.7: 1) 'SRR8440600' was downloaded successfully Read 36221247 spots for SRR8440600/SRR8440600.sra Written 36221247 spots for SRR8440600/SRR8440600.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:36 36221247 reads; of these: 36221247 (100.00%) were unpaired; of these: 2204421 (6.09%) aligned 0 times 29729261 (82.08%) aligned exactly 1 time 4287565 (11.84%) aligned >1 times 93.91% overall alignment rate Time searching: 00:08:36 Overall time: 00:08:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4005804 / 34016826 = 0.1178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:29: 1000000 INFO @ Sun, 21 Jun 2020 21:52:34: 2000000 INFO @ Sun, 21 Jun 2020 21:52:40: 3000000 INFO @ Sun, 21 Jun 2020 21:52:45: 4000000 INFO @ Sun, 21 Jun 2020 21:52:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:56: 6000000 INFO @ Sun, 21 Jun 2020 21:52:59: 1000000 INFO @ Sun, 21 Jun 2020 21:53:02: 7000000 INFO @ Sun, 21 Jun 2020 21:53:05: 2000000 INFO @ Sun, 21 Jun 2020 21:53:07: 8000000 INFO @ Sun, 21 Jun 2020 21:53:11: 3000000 INFO @ Sun, 21 Jun 2020 21:53:13: 9000000 INFO @ Sun, 21 Jun 2020 21:53:16: 4000000 INFO @ Sun, 21 Jun 2020 21:53:18: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:22: 5000000 INFO @ Sun, 21 Jun 2020 21:53:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:24: 11000000 INFO @ Sun, 21 Jun 2020 21:53:28: 6000000 INFO @ Sun, 21 Jun 2020 21:53:29: 1000000 INFO @ Sun, 21 Jun 2020 21:53:30: 12000000 INFO @ Sun, 21 Jun 2020 21:53:34: 7000000 INFO @ Sun, 21 Jun 2020 21:53:35: 2000000 INFO @ Sun, 21 Jun 2020 21:53:36: 13000000 INFO @ Sun, 21 Jun 2020 21:53:40: 8000000 INFO @ Sun, 21 Jun 2020 21:53:40: 3000000 INFO @ Sun, 21 Jun 2020 21:53:42: 14000000 INFO @ Sun, 21 Jun 2020 21:53:46: 9000000 INFO @ Sun, 21 Jun 2020 21:53:46: 4000000 INFO @ Sun, 21 Jun 2020 21:53:48: 15000000 INFO @ Sun, 21 Jun 2020 21:53:51: 5000000 INFO @ Sun, 21 Jun 2020 21:53:52: 10000000 INFO @ Sun, 21 Jun 2020 21:53:54: 16000000 INFO @ Sun, 21 Jun 2020 21:53:57: 6000000 INFO @ Sun, 21 Jun 2020 21:53:58: 11000000 INFO @ Sun, 21 Jun 2020 21:54:00: 17000000 INFO @ Sun, 21 Jun 2020 21:54:02: 7000000 INFO @ Sun, 21 Jun 2020 21:54:03: 12000000 INFO @ Sun, 21 Jun 2020 21:54:06: 18000000 INFO @ Sun, 21 Jun 2020 21:54:08: 8000000 INFO @ Sun, 21 Jun 2020 21:54:09: 13000000 INFO @ Sun, 21 Jun 2020 21:54:12: 19000000 INFO @ Sun, 21 Jun 2020 21:54:13: 9000000 INFO @ Sun, 21 Jun 2020 21:54:15: 14000000 INFO @ Sun, 21 Jun 2020 21:54:18: 20000000 INFO @ Sun, 21 Jun 2020 21:54:18: 10000000 INFO @ Sun, 21 Jun 2020 21:54:21: 15000000 INFO @ Sun, 21 Jun 2020 21:54:24: 11000000 INFO @ Sun, 21 Jun 2020 21:54:24: 21000000 INFO @ Sun, 21 Jun 2020 21:54:27: 16000000 INFO @ Sun, 21 Jun 2020 21:54:29: 12000000 INFO @ Sun, 21 Jun 2020 21:54:30: 22000000 INFO @ Sun, 21 Jun 2020 21:54:33: 17000000 INFO @ Sun, 21 Jun 2020 21:54:35: 13000000 INFO @ Sun, 21 Jun 2020 21:54:36: 23000000 INFO @ Sun, 21 Jun 2020 21:54:39: 18000000 INFO @ Sun, 21 Jun 2020 21:54:40: 14000000 INFO @ Sun, 21 Jun 2020 21:54:42: 24000000 INFO @ Sun, 21 Jun 2020 21:54:45: 19000000 INFO @ Sun, 21 Jun 2020 21:54:46: 15000000 INFO @ Sun, 21 Jun 2020 21:54:48: 25000000 INFO @ Sun, 21 Jun 2020 21:54:51: 16000000 INFO @ Sun, 21 Jun 2020 21:54:51: 20000000 INFO @ Sun, 21 Jun 2020 21:54:54: 26000000 INFO @ Sun, 21 Jun 2020 21:54:56: 17000000 INFO @ Sun, 21 Jun 2020 21:54:57: 21000000 INFO @ Sun, 21 Jun 2020 21:54:59: 27000000 INFO @ Sun, 21 Jun 2020 21:55:02: 18000000 INFO @ Sun, 21 Jun 2020 21:55:03: 22000000 INFO @ Sun, 21 Jun 2020 21:55:05: 28000000 INFO @ Sun, 21 Jun 2020 21:55:07: 19000000 INFO @ Sun, 21 Jun 2020 21:55:09: 23000000 INFO @ Sun, 21 Jun 2020 21:55:11: 29000000 INFO @ Sun, 21 Jun 2020 21:55:13: 20000000 INFO @ Sun, 21 Jun 2020 21:55:14: 24000000 INFO @ Sun, 21 Jun 2020 21:55:17: 30000000 INFO @ Sun, 21 Jun 2020 21:55:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:17: #1 total tags in treatment: 30011022 INFO @ Sun, 21 Jun 2020 21:55:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:18: 21000000 INFO @ Sun, 21 Jun 2020 21:55:18: #1 tags after filtering in treatment: 30010951 INFO @ Sun, 21 Jun 2020 21:55:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:20: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:55:20: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:20: 25000000 INFO @ Sun, 21 Jun 2020 21:55:23: 22000000 INFO @ Sun, 21 Jun 2020 21:55:26: 26000000 INFO @ Sun, 21 Jun 2020 21:55:29: 23000000 INFO @ Sun, 21 Jun 2020 21:55:31: 27000000 INFO @ Sun, 21 Jun 2020 21:55:34: 24000000 INFO @ Sun, 21 Jun 2020 21:55:37: 28000000 INFO @ Sun, 21 Jun 2020 21:55:40: 25000000 INFO @ Sun, 21 Jun 2020 21:55:42: 29000000 INFO @ Sun, 21 Jun 2020 21:55:45: 26000000 INFO @ Sun, 21 Jun 2020 21:55:48: 30000000 INFO @ Sun, 21 Jun 2020 21:55:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:48: #1 total tags in treatment: 30011022 INFO @ Sun, 21 Jun 2020 21:55:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:49: #1 tags after filtering in treatment: 30010951 INFO @ Sun, 21 Jun 2020 21:55:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:50: 27000000 INFO @ Sun, 21 Jun 2020 21:55:51: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:55:51: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:55:56: 28000000 INFO @ Sun, 21 Jun 2020 21:56:01: 29000000 INFO @ Sun, 21 Jun 2020 21:56:06: 30000000 INFO @ Sun, 21 Jun 2020 21:56:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:07: #1 total tags in treatment: 30011022 INFO @ Sun, 21 Jun 2020 21:56:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:07: #1 tags after filtering in treatment: 30010951 INFO @ Sun, 21 Jun 2020 21:56:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:07: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:09: #2 number of paired peaks: 70 WARNING @ Sun, 21 Jun 2020 21:56:09: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5247953/SRX5247953.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。