Job ID = 6458410 SRX = SRX5125697 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:49:37 prefetch.2.10.7: 1) Downloading 'SRR8312315'... 2020-06-21T12:49:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:51:45 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:51:45 prefetch.2.10.7: 1) 'SRR8312315' was downloaded successfully 2020-06-21T12:51:45 prefetch.2.10.7: 'SRR8312315' has 0 unresolved dependencies Read 19651010 spots for SRR8312315/SRR8312315.sra Written 19651010 spots for SRR8312315/SRR8312315.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 19651010 reads; of these: 19651010 (100.00%) were unpaired; of these: 577384 (2.94%) aligned 0 times 15384428 (78.29%) aligned exactly 1 time 3689198 (18.77%) aligned >1 times 97.06% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 975375 / 19073626 = 0.0511 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:05:05: 1000000 INFO @ Sun, 21 Jun 2020 22:05:11: 2000000 INFO @ Sun, 21 Jun 2020 22:05:17: 3000000 INFO @ Sun, 21 Jun 2020 22:05:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:05:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:05:28: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:05:28: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:05:28: 5000000 INFO @ Sun, 21 Jun 2020 22:05:35: 1000000 INFO @ Sun, 21 Jun 2020 22:05:35: 6000000 INFO @ Sun, 21 Jun 2020 22:05:41: 7000000 INFO @ Sun, 21 Jun 2020 22:05:41: 2000000 INFO @ Sun, 21 Jun 2020 22:05:47: 8000000 INFO @ Sun, 21 Jun 2020 22:05:47: 3000000 INFO @ Sun, 21 Jun 2020 22:05:53: 9000000 INFO @ Sun, 21 Jun 2020 22:05:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:05:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:05:58: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:05:58: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:06:00: 10000000 INFO @ Sun, 21 Jun 2020 22:06:00: 5000000 INFO @ Sun, 21 Jun 2020 22:06:05: 1000000 INFO @ Sun, 21 Jun 2020 22:06:07: 11000000 INFO @ Sun, 21 Jun 2020 22:06:07: 6000000 INFO @ Sun, 21 Jun 2020 22:06:12: 2000000 INFO @ Sun, 21 Jun 2020 22:06:13: 12000000 INFO @ Sun, 21 Jun 2020 22:06:14: 7000000 INFO @ Sun, 21 Jun 2020 22:06:19: 3000000 INFO @ Sun, 21 Jun 2020 22:06:20: 13000000 INFO @ Sun, 21 Jun 2020 22:06:21: 8000000 INFO @ Sun, 21 Jun 2020 22:06:26: 4000000 INFO @ Sun, 21 Jun 2020 22:06:27: 14000000 INFO @ Sun, 21 Jun 2020 22:06:28: 9000000 INFO @ Sun, 21 Jun 2020 22:06:33: 5000000 INFO @ Sun, 21 Jun 2020 22:06:34: 10000000 INFO @ Sun, 21 Jun 2020 22:06:35: 15000000 INFO @ Sun, 21 Jun 2020 22:06:40: 6000000 INFO @ Sun, 21 Jun 2020 22:06:41: 11000000 INFO @ Sun, 21 Jun 2020 22:06:42: 16000000 INFO @ Sun, 21 Jun 2020 22:06:46: 7000000 INFO @ Sun, 21 Jun 2020 22:06:48: 12000000 INFO @ Sun, 21 Jun 2020 22:06:49: 17000000 INFO @ Sun, 21 Jun 2020 22:06:53: 8000000 INFO @ Sun, 21 Jun 2020 22:06:55: 13000000 INFO @ Sun, 21 Jun 2020 22:06:56: 18000000 INFO @ Sun, 21 Jun 2020 22:06:56: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:06:56: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:06:56: #1 total tags in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:06:56: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:06:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:06:57: #1 tags after filtering in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:06:57: #1 finished! INFO @ Sun, 21 Jun 2020 22:06:57: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:06:58: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 22:06:58: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:06:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:07:00: 9000000 INFO @ Sun, 21 Jun 2020 22:07:02: 14000000 INFO @ Sun, 21 Jun 2020 22:07:06: 10000000 INFO @ Sun, 21 Jun 2020 22:07:09: 15000000 INFO @ Sun, 21 Jun 2020 22:07:13: 11000000 INFO @ Sun, 21 Jun 2020 22:07:16: 16000000 INFO @ Sun, 21 Jun 2020 22:07:20: 12000000 INFO @ Sun, 21 Jun 2020 22:07:23: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:07:26: 13000000 INFO @ Sun, 21 Jun 2020 22:07:30: 18000000 INFO @ Sun, 21 Jun 2020 22:07:31: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:07:31: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:07:31: #1 total tags in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:07:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:07:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:07:31: #1 tags after filtering in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:07:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:07:31: #1 finished! INFO @ Sun, 21 Jun 2020 22:07:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:07:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:07:32: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 22:07:32: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:07:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:07:33: 14000000 INFO @ Sun, 21 Jun 2020 22:07:40: 15000000 INFO @ Sun, 21 Jun 2020 22:07:46: 16000000 INFO @ Sun, 21 Jun 2020 22:07:52: 17000000 INFO @ Sun, 21 Jun 2020 22:07:58: 18000000 INFO @ Sun, 21 Jun 2020 22:07:59: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 22:07:59: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 22:07:59: #1 total tags in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:07:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:07:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:07:59: #1 tags after filtering in treatment: 18098251 INFO @ Sun, 21 Jun 2020 22:07:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:07:59: #1 finished! INFO @ Sun, 21 Jun 2020 22:07:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:07:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:08:00: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 22:08:00: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:08:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125697/SRX5125697.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。