Job ID = 6458408 SRX = SRX5125695 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:27:22 prefetch.2.10.7: 1) Downloading 'SRR8312313'... 2020-06-21T12:27:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:29:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:29:15 prefetch.2.10.7: 'SRR8312313' is valid 2020-06-21T12:29:15 prefetch.2.10.7: 1) 'SRR8312313' was downloaded successfully 2020-06-21T12:29:15 prefetch.2.10.7: 'SRR8312313' has 0 unresolved dependencies Read 13616003 spots for SRR8312313/SRR8312313.sra Written 13616003 spots for SRR8312313/SRR8312313.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:42 13616003 reads; of these: 13616003 (100.00%) were unpaired; of these: 638391 (4.69%) aligned 0 times 10894463 (80.01%) aligned exactly 1 time 2083149 (15.30%) aligned >1 times 95.31% overall alignment rate Time searching: 00:04:42 Overall time: 00:04:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 789086 / 12977612 = 0.0608 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:38:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:38:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:38:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:38:38: 1000000 INFO @ Sun, 21 Jun 2020 21:38:43: 2000000 INFO @ Sun, 21 Jun 2020 21:38:49: 3000000 INFO @ Sun, 21 Jun 2020 21:38:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:39:02: 5000000 INFO @ Sun, 21 Jun 2020 21:39:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:39:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:39:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:39:08: 1000000 INFO @ Sun, 21 Jun 2020 21:39:08: 6000000 INFO @ Sun, 21 Jun 2020 21:39:15: 2000000 INFO @ Sun, 21 Jun 2020 21:39:15: 7000000 INFO @ Sun, 21 Jun 2020 21:39:21: 8000000 INFO @ Sun, 21 Jun 2020 21:39:21: 3000000 INFO @ Sun, 21 Jun 2020 21:39:27: 9000000 INFO @ Sun, 21 Jun 2020 21:39:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:39:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:39:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:39:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:39:34: 10000000 INFO @ Sun, 21 Jun 2020 21:39:35: 5000000 INFO @ Sun, 21 Jun 2020 21:39:38: 1000000 INFO @ Sun, 21 Jun 2020 21:39:41: 11000000 INFO @ Sun, 21 Jun 2020 21:39:42: 6000000 INFO @ Sun, 21 Jun 2020 21:39:45: 2000000 INFO @ Sun, 21 Jun 2020 21:39:47: 12000000 INFO @ Sun, 21 Jun 2020 21:39:48: 7000000 INFO @ Sun, 21 Jun 2020 21:39:49: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:39:49: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:39:49: #1 total tags in treatment: 12188526 INFO @ Sun, 21 Jun 2020 21:39:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:39:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:39:49: #1 tags after filtering in treatment: 12188522 INFO @ Sun, 21 Jun 2020 21:39:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:39:49: #1 finished! INFO @ Sun, 21 Jun 2020 21:39:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:39:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:39:50: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:39:50: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:39:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:39:51: 3000000 INFO @ Sun, 21 Jun 2020 21:39:54: 8000000 INFO @ Sun, 21 Jun 2020 21:39:58: 4000000 INFO @ Sun, 21 Jun 2020 21:40:01: 9000000 INFO @ Sun, 21 Jun 2020 21:40:05: 5000000 INFO @ Sun, 21 Jun 2020 21:40:07: 10000000 INFO @ Sun, 21 Jun 2020 21:40:11: 6000000 INFO @ Sun, 21 Jun 2020 21:40:14: 11000000 INFO @ Sun, 21 Jun 2020 21:40:18: 7000000 INFO @ Sun, 21 Jun 2020 21:40:20: 12000000 INFO @ Sun, 21 Jun 2020 21:40:21: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:40:21: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:40:21: #1 total tags in treatment: 12188526 INFO @ Sun, 21 Jun 2020 21:40:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:40:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:40:22: #1 tags after filtering in treatment: 12188522 INFO @ Sun, 21 Jun 2020 21:40:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:40:22: #1 finished! INFO @ Sun, 21 Jun 2020 21:40:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:40:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:40:23: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:40:23: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:40:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:40:24: 8000000 INFO @ Sun, 21 Jun 2020 21:40:30: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:40:36: 10000000 INFO @ Sun, 21 Jun 2020 21:40:42: 11000000 INFO @ Sun, 21 Jun 2020 21:40:48: 12000000 INFO @ Sun, 21 Jun 2020 21:40:50: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:40:50: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:40:50: #1 total tags in treatment: 12188526 INFO @ Sun, 21 Jun 2020 21:40:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:40:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:40:50: #1 tags after filtering in treatment: 12188522 INFO @ Sun, 21 Jun 2020 21:40:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:40:50: #1 finished! INFO @ Sun, 21 Jun 2020 21:40:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:40:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:40:51: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 21:40:51: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:40:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125695/SRX5125695.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。