Job ID = 6458407 SRX = SRX5125694 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:34:22 prefetch.2.10.7: 1) Downloading 'SRR8312312'... 2020-06-21T12:34:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:37:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:37:05 prefetch.2.10.7: 'SRR8312312' is valid 2020-06-21T12:37:05 prefetch.2.10.7: 1) 'SRR8312312' was downloaded successfully 2020-06-21T12:37:05 prefetch.2.10.7: 'SRR8312312' has 0 unresolved dependencies Read 16416905 spots for SRR8312312/SRR8312312.sra Written 16416905 spots for SRR8312312/SRR8312312.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:42 16416905 reads; of these: 16416905 (100.00%) were unpaired; of these: 1666692 (10.15%) aligned 0 times 12682137 (77.25%) aligned exactly 1 time 2068076 (12.60%) aligned >1 times 89.85% overall alignment rate Time searching: 00:05:42 Overall time: 00:05:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 877660 / 14750213 = 0.0595 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:48:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:48:06: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:48:06: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:48:13: 1000000 INFO @ Sun, 21 Jun 2020 21:48:19: 2000000 INFO @ Sun, 21 Jun 2020 21:48:25: 3000000 INFO @ Sun, 21 Jun 2020 21:48:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:48:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:48:37: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:48:37: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:48:38: 5000000 INFO @ Sun, 21 Jun 2020 21:48:44: 1000000 INFO @ Sun, 21 Jun 2020 21:48:45: 6000000 INFO @ Sun, 21 Jun 2020 21:48:51: 2000000 INFO @ Sun, 21 Jun 2020 21:48:52: 7000000 INFO @ Sun, 21 Jun 2020 21:48:58: 3000000 INFO @ Sun, 21 Jun 2020 21:48:59: 8000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 21:49:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:49:06: 9000000 INFO @ Sun, 21 Jun 2020 21:49:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:49:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:49:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:49:12: 5000000 INFO @ Sun, 21 Jun 2020 21:49:13: 10000000 INFO @ Sun, 21 Jun 2020 21:49:14: 1000000 INFO @ Sun, 21 Jun 2020 21:49:19: 6000000 INFO @ Sun, 21 Jun 2020 21:49:21: 11000000 INFO @ Sun, 21 Jun 2020 21:49:21: 2000000 INFO @ Sun, 21 Jun 2020 21:49:26: 7000000 INFO @ Sun, 21 Jun 2020 21:49:28: 12000000 INFO @ Sun, 21 Jun 2020 21:49:28: 3000000 INFO @ Sun, 21 Jun 2020 21:49:33: 8000000 INFO @ Sun, 21 Jun 2020 21:49:36: 13000000 INFO @ Sun, 21 Jun 2020 21:49:36: 4000000 INFO @ Sun, 21 Jun 2020 21:49:40: 9000000 INFO @ Sun, 21 Jun 2020 21:49:42: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:49:42: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:49:42: #1 total tags in treatment: 13872553 INFO @ Sun, 21 Jun 2020 21:49:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:49:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:49:43: #1 tags after filtering in treatment: 13872548 INFO @ Sun, 21 Jun 2020 21:49:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:49:43: #1 finished! INFO @ Sun, 21 Jun 2020 21:49:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:49:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:49:43: 5000000 INFO @ Sun, 21 Jun 2020 21:49:44: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 21:49:44: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:49:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:49:47: 10000000 INFO @ Sun, 21 Jun 2020 21:49:50: 6000000 INFO @ Sun, 21 Jun 2020 21:49:54: 11000000 INFO @ Sun, 21 Jun 2020 21:49:58: 7000000 INFO @ Sun, 21 Jun 2020 21:50:02: 12000000 INFO @ Sun, 21 Jun 2020 21:50:05: 8000000 INFO @ Sun, 21 Jun 2020 21:50:09: 13000000 INFO @ Sun, 21 Jun 2020 21:50:13: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:50:16: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:50:16: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:50:16: #1 total tags in treatment: 13872553 INFO @ Sun, 21 Jun 2020 21:50:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:16: #1 tags after filtering in treatment: 13872548 INFO @ Sun, 21 Jun 2020 21:50:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:17: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 21:50:17: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:50:19: 10000000 INFO @ Sun, 21 Jun 2020 21:50:26: 11000000 INFO @ Sun, 21 Jun 2020 21:50:33: 12000000 INFO @ Sun, 21 Jun 2020 21:50:40: 13000000 INFO @ Sun, 21 Jun 2020 21:50:46: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 21:50:46: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 21:50:46: #1 total tags in treatment: 13872553 INFO @ Sun, 21 Jun 2020 21:50:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:46: #1 tags after filtering in treatment: 13872548 INFO @ Sun, 21 Jun 2020 21:50:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:46: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:47: #2 number of paired peaks: 82 WARNING @ Sun, 21 Jun 2020 21:50:47: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5125694/SRX5125694.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。