Job ID = 6458405 SRX = SRX512268 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:26:34 prefetch.2.10.7: 1) Downloading 'SRR1220294'... 2020-06-21T12:26:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:34:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:34:37 prefetch.2.10.7: 1) 'SRR1220294' was downloaded successfully Read 66782738 spots for SRR1220294/SRR1220294.sra Written 66782738 spots for SRR1220294/SRR1220294.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:47 66782738 reads; of these: 66782738 (100.00%) were unpaired; of these: 5726870 (8.58%) aligned 0 times 53288043 (79.79%) aligned exactly 1 time 7767825 (11.63%) aligned >1 times 91.42% overall alignment rate Time searching: 00:13:47 Overall time: 00:13:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 19305235 / 61055868 = 0.3162 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:03:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:03:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:03:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:03:59: 1000000 INFO @ Sun, 21 Jun 2020 22:04:06: 2000000 INFO @ Sun, 21 Jun 2020 22:04:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:21: 4000000 INFO @ Sun, 21 Jun 2020 22:04:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:04:28: 5000000 INFO @ Sun, 21 Jun 2020 22:04:30: 1000000 INFO @ Sun, 21 Jun 2020 22:04:37: 2000000 INFO @ Sun, 21 Jun 2020 22:04:37: 6000000 INFO @ Sun, 21 Jun 2020 22:04:44: 3000000 INFO @ Sun, 21 Jun 2020 22:04:47: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:51: 4000000 INFO @ Sun, 21 Jun 2020 22:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:04:56: 8000000 INFO @ Sun, 21 Jun 2020 22:04:59: 5000000 INFO @ Sun, 21 Jun 2020 22:05:00: 1000000 INFO @ Sun, 21 Jun 2020 22:05:06: 9000000 INFO @ Sun, 21 Jun 2020 22:05:06: 6000000 INFO @ Sun, 21 Jun 2020 22:05:07: 2000000 INFO @ Sun, 21 Jun 2020 22:05:13: 7000000 INFO @ Sun, 21 Jun 2020 22:05:14: 3000000 INFO @ Sun, 21 Jun 2020 22:05:15: 10000000 INFO @ Sun, 21 Jun 2020 22:05:21: 8000000 INFO @ Sun, 21 Jun 2020 22:05:22: 4000000 INFO @ Sun, 21 Jun 2020 22:05:25: 11000000 INFO @ Sun, 21 Jun 2020 22:05:28: 9000000 INFO @ Sun, 21 Jun 2020 22:05:29: 5000000 INFO @ Sun, 21 Jun 2020 22:05:35: 12000000 INFO @ Sun, 21 Jun 2020 22:05:36: 10000000 INFO @ Sun, 21 Jun 2020 22:05:37: 6000000 INFO @ Sun, 21 Jun 2020 22:05:43: 11000000 INFO @ Sun, 21 Jun 2020 22:05:44: 7000000 INFO @ Sun, 21 Jun 2020 22:05:45: 13000000 INFO @ Sun, 21 Jun 2020 22:05:51: 12000000 INFO @ Sun, 21 Jun 2020 22:05:52: 8000000 INFO @ Sun, 21 Jun 2020 22:05:54: 14000000 INFO @ Sun, 21 Jun 2020 22:05:58: 13000000 INFO @ Sun, 21 Jun 2020 22:05:59: 9000000 INFO @ Sun, 21 Jun 2020 22:06:04: 15000000 INFO @ Sun, 21 Jun 2020 22:06:05: 14000000 INFO @ Sun, 21 Jun 2020 22:06:06: 10000000 INFO @ Sun, 21 Jun 2020 22:06:13: 15000000 INFO @ Sun, 21 Jun 2020 22:06:14: 11000000 INFO @ Sun, 21 Jun 2020 22:06:14: 16000000 INFO @ Sun, 21 Jun 2020 22:06:20: 16000000 INFO @ Sun, 21 Jun 2020 22:06:21: 12000000 INFO @ Sun, 21 Jun 2020 22:06:23: 17000000 INFO @ Sun, 21 Jun 2020 22:06:28: 17000000 INFO @ Sun, 21 Jun 2020 22:06:29: 13000000 INFO @ Sun, 21 Jun 2020 22:06:33: 18000000 INFO @ Sun, 21 Jun 2020 22:06:35: 18000000 INFO @ Sun, 21 Jun 2020 22:06:36: 14000000 INFO @ Sun, 21 Jun 2020 22:06:42: 19000000 INFO @ Sun, 21 Jun 2020 22:06:43: 19000000 INFO @ Sun, 21 Jun 2020 22:06:43: 15000000 INFO @ Sun, 21 Jun 2020 22:06:51: 16000000 INFO @ Sun, 21 Jun 2020 22:06:51: 20000000 INFO @ Sun, 21 Jun 2020 22:06:52: 20000000 INFO @ Sun, 21 Jun 2020 22:06:58: 17000000 INFO @ Sun, 21 Jun 2020 22:07:00: 21000000 INFO @ Sun, 21 Jun 2020 22:07:01: 21000000 INFO @ Sun, 21 Jun 2020 22:07:06: 18000000 INFO @ Sun, 21 Jun 2020 22:07:09: 22000000 INFO @ Sun, 21 Jun 2020 22:07:10: 22000000 INFO @ Sun, 21 Jun 2020 22:07:13: 19000000 INFO @ Sun, 21 Jun 2020 22:07:17: 23000000 INFO @ Sun, 21 Jun 2020 22:07:18: 23000000 INFO @ Sun, 21 Jun 2020 22:07:21: 20000000 INFO @ Sun, 21 Jun 2020 22:07:25: 24000000 INFO @ Sun, 21 Jun 2020 22:07:27: 24000000 INFO @ Sun, 21 Jun 2020 22:07:28: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:07:33: 25000000 INFO @ Sun, 21 Jun 2020 22:07:35: 25000000 INFO @ Sun, 21 Jun 2020 22:07:36: 22000000 INFO @ Sun, 21 Jun 2020 22:07:41: 26000000 INFO @ Sun, 21 Jun 2020 22:07:43: 26000000 INFO @ Sun, 21 Jun 2020 22:07:44: 23000000 INFO @ Sun, 21 Jun 2020 22:07:48: 27000000 INFO @ Sun, 21 Jun 2020 22:07:52: 27000000 INFO @ Sun, 21 Jun 2020 22:07:52: 24000000 INFO @ Sun, 21 Jun 2020 22:07:56: 28000000 INFO @ Sun, 21 Jun 2020 22:07:59: 25000000 INFO @ Sun, 21 Jun 2020 22:08:00: 28000000 INFO @ Sun, 21 Jun 2020 22:08:03: 29000000 INFO @ Sun, 21 Jun 2020 22:08:07: 26000000 INFO @ Sun, 21 Jun 2020 22:08:08: 29000000 INFO @ Sun, 21 Jun 2020 22:08:11: 30000000 INFO @ Sun, 21 Jun 2020 22:08:14: 27000000 INFO @ Sun, 21 Jun 2020 22:08:16: 30000000 INFO @ Sun, 21 Jun 2020 22:08:19: 31000000 INFO @ Sun, 21 Jun 2020 22:08:22: 28000000 INFO @ Sun, 21 Jun 2020 22:08:24: 31000000 INFO @ Sun, 21 Jun 2020 22:08:26: 32000000 INFO @ Sun, 21 Jun 2020 22:08:29: 29000000 INFO @ Sun, 21 Jun 2020 22:08:32: 32000000 INFO @ Sun, 21 Jun 2020 22:08:34: 33000000 INFO @ Sun, 21 Jun 2020 22:08:37: 30000000 INFO @ Sun, 21 Jun 2020 22:08:40: 33000000 INFO @ Sun, 21 Jun 2020 22:08:41: 34000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:08:44: 31000000 INFO @ Sun, 21 Jun 2020 22:08:48: 34000000 INFO @ Sun, 21 Jun 2020 22:08:49: 35000000 INFO @ Sun, 21 Jun 2020 22:08:52: 32000000 INFO @ Sun, 21 Jun 2020 22:08:56: 35000000 INFO @ Sun, 21 Jun 2020 22:08:57: 36000000 INFO @ Sun, 21 Jun 2020 22:08:59: 33000000 INFO @ Sun, 21 Jun 2020 22:09:04: 36000000 INFO @ Sun, 21 Jun 2020 22:09:04: 37000000 INFO @ Sun, 21 Jun 2020 22:09:07: 34000000 INFO @ Sun, 21 Jun 2020 22:09:11: 38000000 INFO @ Sun, 21 Jun 2020 22:09:12: 37000000 INFO @ Sun, 21 Jun 2020 22:09:15: 35000000 INFO @ Sun, 21 Jun 2020 22:09:19: 39000000 INFO @ Sun, 21 Jun 2020 22:09:20: 38000000 INFO @ Sun, 21 Jun 2020 22:09:22: 36000000 INFO @ Sun, 21 Jun 2020 22:09:26: 40000000 INFO @ Sun, 21 Jun 2020 22:09:28: 39000000 INFO @ Sun, 21 Jun 2020 22:09:30: 37000000 INFO @ Sun, 21 Jun 2020 22:09:34: 41000000 INFO @ Sun, 21 Jun 2020 22:09:35: 40000000 INFO @ Sun, 21 Jun 2020 22:09:37: 38000000 INFO @ Sun, 21 Jun 2020 22:09:40: #1 tag size is determined as 46 bps INFO @ Sun, 21 Jun 2020 22:09:40: #1 tag size = 46 INFO @ Sun, 21 Jun 2020 22:09:40: #1 total tags in treatment: 41750633 INFO @ Sun, 21 Jun 2020 22:09:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:09:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:09:41: #1 tags after filtering in treatment: 41750632 INFO @ Sun, 21 Jun 2020 22:09:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:09:41: #1 finished! INFO @ Sun, 21 Jun 2020 22:09:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:09:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:09:43: 41000000 INFO @ Sun, 21 Jun 2020 22:09:44: #2 number of paired peaks: 18 WARNING @ Sun, 21 Jun 2020 22:09:44: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:09:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:09:45: 39000000 INFO @ Sun, 21 Jun 2020 22:09:50: #1 tag size is determined as 46 bps INFO @ Sun, 21 Jun 2020 22:09:50: #1 tag size = 46 INFO @ Sun, 21 Jun 2020 22:09:50: #1 total tags in treatment: 41750633 INFO @ Sun, 21 Jun 2020 22:09:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:09:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:09:50: #1 tags after filtering in treatment: 41750632 INFO @ Sun, 21 Jun 2020 22:09:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:09:50: #1 finished! INFO @ Sun, 21 Jun 2020 22:09:50: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:09:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:09:52: 40000000 INFO @ Sun, 21 Jun 2020 22:09:53: #2 number of paired peaks: 18 WARNING @ Sun, 21 Jun 2020 22:09:53: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:09:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:09:59: 41000000 INFO @ Sun, 21 Jun 2020 22:10:04: #1 tag size is determined as 46 bps INFO @ Sun, 21 Jun 2020 22:10:04: #1 tag size = 46 INFO @ Sun, 21 Jun 2020 22:10:04: #1 total tags in treatment: 41750633 INFO @ Sun, 21 Jun 2020 22:10:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:10:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:10:05: #1 tags after filtering in treatment: 41750632 INFO @ Sun, 21 Jun 2020 22:10:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:10:05: #1 finished! INFO @ Sun, 21 Jun 2020 22:10:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:10:07: #2 number of paired peaks: 18 WARNING @ Sun, 21 Jun 2020 22:10:07: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:10:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX512268/SRX512268.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling