Job ID = 12265422 SRX = SRX5010751 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 20645066 reads; of these: 20645066 (100.00%) were unpaired; of these: 797642 (3.86%) aligned 0 times 15925801 (77.14%) aligned exactly 1 time 3921623 (19.00%) aligned >1 times 96.14% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10594769 / 19847424 = 0.5338 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:07:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:07:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:07:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:07:41: 1000000 INFO @ Sat, 03 Apr 2021 07:07:50: 2000000 INFO @ Sat, 03 Apr 2021 07:07:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:08:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:08:03: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:08:03: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:08:05: 4000000 INFO @ Sat, 03 Apr 2021 07:08:10: 1000000 INFO @ Sat, 03 Apr 2021 07:08:12: 5000000 INFO @ Sat, 03 Apr 2021 07:08:17: 2000000 INFO @ Sat, 03 Apr 2021 07:08:19: 6000000 INFO @ Sat, 03 Apr 2021 07:08:24: 3000000 INFO @ Sat, 03 Apr 2021 07:08:26: 7000000 INFO @ Sat, 03 Apr 2021 07:08:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:08:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:08:33: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:08:33: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:08:33: 8000000 INFO @ Sat, 03 Apr 2021 07:08:37: 5000000 INFO @ Sat, 03 Apr 2021 07:08:40: 1000000 INFO @ Sat, 03 Apr 2021 07:08:40: 9000000 INFO @ Sat, 03 Apr 2021 07:08:42: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:08:42: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:08:42: #1 total tags in treatment: 9252655 INFO @ Sat, 03 Apr 2021 07:08:42: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:08:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:08:43: #1 tags after filtering in treatment: 9252548 INFO @ Sat, 03 Apr 2021 07:08:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:08:43: #1 finished! INFO @ Sat, 03 Apr 2021 07:08:43: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:08:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:08:44: #2 number of paired peaks: 1242 INFO @ Sat, 03 Apr 2021 07:08:44: start model_add_line... INFO @ Sat, 03 Apr 2021 07:08:44: start X-correlation... INFO @ Sat, 03 Apr 2021 07:08:44: end of X-cor INFO @ Sat, 03 Apr 2021 07:08:44: #2 finished! INFO @ Sat, 03 Apr 2021 07:08:44: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 07:08:44: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 07:08:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05_model.r WARNING @ Sat, 03 Apr 2021 07:08:44: #2 Since the d (73) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:08:44: #2 You may need to consider one of the other alternative d(s): 73 WARNING @ Sat, 03 Apr 2021 07:08:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:08:44: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:08:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:08:45: 6000000 INFO @ Sat, 03 Apr 2021 07:08:47: 2000000 INFO @ Sat, 03 Apr 2021 07:08:52: 7000000 INFO @ Sat, 03 Apr 2021 07:08:54: 3000000 INFO @ Sat, 03 Apr 2021 07:09:00: 8000000 INFO @ Sat, 03 Apr 2021 07:09:01: 4000000 INFO @ Sat, 03 Apr 2021 07:09:03: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:09:08: 9000000 INFO @ Sat, 03 Apr 2021 07:09:09: 5000000 INFO @ Sat, 03 Apr 2021 07:09:10: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:09:10: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:09:10: #1 total tags in treatment: 9252655 INFO @ Sat, 03 Apr 2021 07:09:10: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:09:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:09:10: #1 tags after filtering in treatment: 9252548 INFO @ Sat, 03 Apr 2021 07:09:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:09:10: #1 finished! INFO @ Sat, 03 Apr 2021 07:09:10: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:09:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:09:11: #2 number of paired peaks: 1242 INFO @ Sat, 03 Apr 2021 07:09:11: start model_add_line... INFO @ Sat, 03 Apr 2021 07:09:11: start X-correlation... INFO @ Sat, 03 Apr 2021 07:09:11: end of X-cor INFO @ Sat, 03 Apr 2021 07:09:11: #2 finished! INFO @ Sat, 03 Apr 2021 07:09:11: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 07:09:11: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 07:09:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10_model.r WARNING @ Sat, 03 Apr 2021 07:09:11: #2 Since the d (73) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:09:11: #2 You may need to consider one of the other alternative d(s): 73 WARNING @ Sat, 03 Apr 2021 07:09:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:09:11: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:09:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:09:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:09:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:09:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.05_summits.bed INFO @ Sat, 03 Apr 2021 07:09:14: Done! pass1 - making usageList (400 chroms): 2 millis pass2 - checking and writing primary data (8699 records, 4 fields): 33 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:09:16: 6000000 INFO @ Sat, 03 Apr 2021 07:09:24: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:09:32: 8000000 INFO @ Sat, 03 Apr 2021 07:09:32: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:09:39: 9000000 INFO @ Sat, 03 Apr 2021 07:09:42: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:09:42: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:09:42: #1 total tags in treatment: 9252655 INFO @ Sat, 03 Apr 2021 07:09:42: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:09:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:09:42: #1 tags after filtering in treatment: 9252548 INFO @ Sat, 03 Apr 2021 07:09:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:09:42: #1 finished! INFO @ Sat, 03 Apr 2021 07:09:42: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:09:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:09:43: #2 number of paired peaks: 1242 INFO @ Sat, 03 Apr 2021 07:09:43: start model_add_line... INFO @ Sat, 03 Apr 2021 07:09:43: start X-correlation... INFO @ Sat, 03 Apr 2021 07:09:43: end of X-cor INFO @ Sat, 03 Apr 2021 07:09:43: #2 finished! INFO @ Sat, 03 Apr 2021 07:09:43: #2 predicted fragment length is 73 bps INFO @ Sat, 03 Apr 2021 07:09:43: #2 alternative fragment length(s) may be 73 bps INFO @ Sat, 03 Apr 2021 07:09:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20_model.r WARNING @ Sat, 03 Apr 2021 07:09:43: #2 Since the d (73) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:09:43: #2 You may need to consider one of the other alternative d(s): 73 WARNING @ Sat, 03 Apr 2021 07:09:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:09:43: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:09:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:09:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:09:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:09:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.10_summits.bed INFO @ Sat, 03 Apr 2021 07:09:44: Done! pass1 - making usageList (279 chroms): 10 millis pass2 - checking and writing primary data (4329 records, 4 fields): 32 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:10:02: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:10:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:10:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:10:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5010751/SRX5010751.20_summits.bed INFO @ Sat, 03 Apr 2021 07:10:14: Done! pass1 - making usageList (130 chroms): 2 millis pass2 - checking and writing primary data (1364 records, 4 fields): 14 millis CompletedMACS2peakCalling