Job ID = 12265561 SRX = SRX5010726 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:17 131356618 reads; of these: 131356618 (100.00%) were unpaired; of these: 3041798 (2.32%) aligned 0 times 103959372 (79.14%) aligned exactly 1 time 24355448 (18.54%) aligned >1 times 97.68% overall alignment rate Time searching: 00:31:17 Overall time: 00:31:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 56 files... [bam_rmdupse_core] 84068144 / 128314820 = 0.6552 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:28:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:28:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:28:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:28:49: 1000000 INFO @ Sat, 03 Apr 2021 08:28:56: 2000000 INFO @ Sat, 03 Apr 2021 08:29:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:29:10: 4000000 INFO @ Sat, 03 Apr 2021 08:29:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:29:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:29:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:29:18: 5000000 INFO @ Sat, 03 Apr 2021 08:29:23: 1000000 INFO @ Sat, 03 Apr 2021 08:29:26: 6000000 INFO @ Sat, 03 Apr 2021 08:29:33: 7000000 INFO @ Sat, 03 Apr 2021 08:29:33: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:29:40: 8000000 INFO @ Sat, 03 Apr 2021 08:29:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:29:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:29:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:29:44: 3000000 INFO @ Sat, 03 Apr 2021 08:29:49: 9000000 INFO @ Sat, 03 Apr 2021 08:29:54: 1000000 INFO @ Sat, 03 Apr 2021 08:29:55: 4000000 INFO @ Sat, 03 Apr 2021 08:29:56: 10000000 INFO @ Sat, 03 Apr 2021 08:30:04: 11000000 INFO @ Sat, 03 Apr 2021 08:30:05: 2000000 INFO @ Sat, 03 Apr 2021 08:30:05: 5000000 INFO @ Sat, 03 Apr 2021 08:30:12: 12000000 INFO @ Sat, 03 Apr 2021 08:30:16: 3000000 INFO @ Sat, 03 Apr 2021 08:30:17: 6000000 INFO @ Sat, 03 Apr 2021 08:30:20: 13000000 INFO @ Sat, 03 Apr 2021 08:30:27: 4000000 INFO @ Sat, 03 Apr 2021 08:30:27: 14000000 INFO @ Sat, 03 Apr 2021 08:30:28: 7000000 INFO @ Sat, 03 Apr 2021 08:30:35: 15000000 INFO @ Sat, 03 Apr 2021 08:30:38: 5000000 INFO @ Sat, 03 Apr 2021 08:30:39: 8000000 INFO @ Sat, 03 Apr 2021 08:30:43: 16000000 INFO @ Sat, 03 Apr 2021 08:30:49: 6000000 INFO @ Sat, 03 Apr 2021 08:30:50: 9000000 INFO @ Sat, 03 Apr 2021 08:30:52: 17000000 INFO @ Sat, 03 Apr 2021 08:30:59: 18000000 INFO @ Sat, 03 Apr 2021 08:31:01: 7000000 INFO @ Sat, 03 Apr 2021 08:31:01: 10000000 INFO @ Sat, 03 Apr 2021 08:31:07: 19000000 INFO @ Sat, 03 Apr 2021 08:31:11: 8000000 INFO @ Sat, 03 Apr 2021 08:31:12: 11000000 INFO @ Sat, 03 Apr 2021 08:31:15: 20000000 INFO @ Sat, 03 Apr 2021 08:31:22: 9000000 INFO @ Sat, 03 Apr 2021 08:31:23: 12000000 INFO @ Sat, 03 Apr 2021 08:31:23: 21000000 INFO @ Sat, 03 Apr 2021 08:31:30: 22000000 INFO @ Sat, 03 Apr 2021 08:31:33: 10000000 INFO @ Sat, 03 Apr 2021 08:31:33: 13000000 INFO @ Sat, 03 Apr 2021 08:31:38: 23000000 INFO @ Sat, 03 Apr 2021 08:31:44: 11000000 INFO @ Sat, 03 Apr 2021 08:31:44: 14000000 INFO @ Sat, 03 Apr 2021 08:31:46: 24000000 INFO @ Sat, 03 Apr 2021 08:31:54: 25000000 INFO @ Sat, 03 Apr 2021 08:31:55: 12000000 INFO @ Sat, 03 Apr 2021 08:31:55: 15000000 INFO @ Sat, 03 Apr 2021 08:32:02: 26000000 INFO @ Sat, 03 Apr 2021 08:32:05: 13000000 INFO @ Sat, 03 Apr 2021 08:32:06: 16000000 INFO @ Sat, 03 Apr 2021 08:32:09: 27000000 INFO @ Sat, 03 Apr 2021 08:32:16: 14000000 INFO @ Sat, 03 Apr 2021 08:32:17: 17000000 INFO @ Sat, 03 Apr 2021 08:32:18: 28000000 INFO @ Sat, 03 Apr 2021 08:32:26: 29000000 INFO @ Sat, 03 Apr 2021 08:32:27: 15000000 INFO @ Sat, 03 Apr 2021 08:32:27: 18000000 INFO @ Sat, 03 Apr 2021 08:32:34: 30000000 INFO @ Sat, 03 Apr 2021 08:32:38: 16000000 INFO @ Sat, 03 Apr 2021 08:32:38: 19000000 INFO @ Sat, 03 Apr 2021 08:32:41: 31000000 INFO @ Sat, 03 Apr 2021 08:32:48: 32000000 INFO @ Sat, 03 Apr 2021 08:32:48: 17000000 INFO @ Sat, 03 Apr 2021 08:32:49: 20000000 INFO @ Sat, 03 Apr 2021 08:32:56: 33000000 INFO @ Sat, 03 Apr 2021 08:32:59: 18000000 INFO @ Sat, 03 Apr 2021 08:33:00: 21000000 INFO @ Sat, 03 Apr 2021 08:33:04: 34000000 INFO @ Sat, 03 Apr 2021 08:33:10: 19000000 INFO @ Sat, 03 Apr 2021 08:33:10: 22000000 INFO @ Sat, 03 Apr 2021 08:33:12: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:33:20: 36000000 INFO @ Sat, 03 Apr 2021 08:33:21: 20000000 INFO @ Sat, 03 Apr 2021 08:33:21: 23000000 INFO @ Sat, 03 Apr 2021 08:33:28: 37000000 INFO @ Sat, 03 Apr 2021 08:33:31: 21000000 INFO @ Sat, 03 Apr 2021 08:33:32: 24000000 INFO @ Sat, 03 Apr 2021 08:33:35: 38000000 INFO @ Sat, 03 Apr 2021 08:33:41: 22000000 INFO @ Sat, 03 Apr 2021 08:33:42: 25000000 INFO @ Sat, 03 Apr 2021 08:33:42: 39000000 INFO @ Sat, 03 Apr 2021 08:33:50: 40000000 INFO @ Sat, 03 Apr 2021 08:33:51: 23000000 INFO @ Sat, 03 Apr 2021 08:33:52: 26000000 INFO @ Sat, 03 Apr 2021 08:33:58: 41000000 INFO @ Sat, 03 Apr 2021 08:34:01: 24000000 INFO @ Sat, 03 Apr 2021 08:34:01: 27000000 INFO @ Sat, 03 Apr 2021 08:34:05: 42000000 INFO @ Sat, 03 Apr 2021 08:34:10: 25000000 INFO @ Sat, 03 Apr 2021 08:34:11: 28000000 INFO @ Sat, 03 Apr 2021 08:34:13: 43000000 INFO @ Sat, 03 Apr 2021 08:34:19: 26000000 INFO @ Sat, 03 Apr 2021 08:34:20: 29000000 INFO @ Sat, 03 Apr 2021 08:34:21: 44000000 INFO @ Sat, 03 Apr 2021 08:34:23: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:34:23: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:34:23: #1 total tags in treatment: 44246676 INFO @ Sat, 03 Apr 2021 08:34:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:34:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:34:24: #1 tags after filtering in treatment: 44246647 INFO @ Sat, 03 Apr 2021 08:34:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:34:24: #1 finished! INFO @ Sat, 03 Apr 2021 08:34:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:34:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:34:27: #2 number of paired peaks: 16 WARNING @ Sat, 03 Apr 2021 08:34:27: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:34:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:34:28: 27000000 INFO @ Sat, 03 Apr 2021 08:34:28: 30000000 INFO @ Sat, 03 Apr 2021 08:34:37: 28000000 INFO @ Sat, 03 Apr 2021 08:34:37: 31000000 INFO @ Sat, 03 Apr 2021 08:34:46: 32000000 INFO @ Sat, 03 Apr 2021 08:34:46: 29000000 INFO @ Sat, 03 Apr 2021 08:34:55: 33000000 INFO @ Sat, 03 Apr 2021 08:34:55: 30000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:35:04: 34000000 INFO @ Sat, 03 Apr 2021 08:35:04: 31000000 INFO @ Sat, 03 Apr 2021 08:35:13: 32000000 INFO @ Sat, 03 Apr 2021 08:35:14: 35000000 INFO @ Sat, 03 Apr 2021 08:35:22: 33000000 INFO @ Sat, 03 Apr 2021 08:35:23: 36000000 INFO @ Sat, 03 Apr 2021 08:35:32: 34000000 INFO @ Sat, 03 Apr 2021 08:35:33: 37000000 INFO @ Sat, 03 Apr 2021 08:35:42: 35000000 INFO @ Sat, 03 Apr 2021 08:35:42: 38000000 INFO @ Sat, 03 Apr 2021 08:35:51: 36000000 INFO @ Sat, 03 Apr 2021 08:35:51: 39000000 INFO @ Sat, 03 Apr 2021 08:36:00: 37000000 INFO @ Sat, 03 Apr 2021 08:36:00: 40000000 INFO @ Sat, 03 Apr 2021 08:36:09: 41000000 INFO @ Sat, 03 Apr 2021 08:36:09: 38000000 INFO @ Sat, 03 Apr 2021 08:36:18: 42000000 INFO @ Sat, 03 Apr 2021 08:36:18: 39000000 INFO @ Sat, 03 Apr 2021 08:36:28: 40000000 INFO @ Sat, 03 Apr 2021 08:36:28: 43000000 INFO @ Sat, 03 Apr 2021 08:36:37: 41000000 INFO @ Sat, 03 Apr 2021 08:36:38: 44000000 INFO @ Sat, 03 Apr 2021 08:36:40: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:36:40: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:36:40: #1 total tags in treatment: 44246676 INFO @ Sat, 03 Apr 2021 08:36:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:36:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:36:42: #1 tags after filtering in treatment: 44246647 INFO @ Sat, 03 Apr 2021 08:36:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:36:42: #1 finished! INFO @ Sat, 03 Apr 2021 08:36:42: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:36:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:36:44: #2 number of paired peaks: 16 WARNING @ Sat, 03 Apr 2021 08:36:44: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:36:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:36:46: 42000000 INFO @ Sat, 03 Apr 2021 08:36:56: 43000000 INFO @ Sat, 03 Apr 2021 08:37:05: 44000000 INFO @ Sat, 03 Apr 2021 08:37:08: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:37:08: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:37:08: #1 total tags in treatment: 44246676 INFO @ Sat, 03 Apr 2021 08:37:08: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:37:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:37:09: #1 tags after filtering in treatment: 44246647 INFO @ Sat, 03 Apr 2021 08:37:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:37:09: #1 finished! INFO @ Sat, 03 Apr 2021 08:37:09: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:37:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:37:12: #2 number of paired peaks: 16 WARNING @ Sat, 03 Apr 2021 08:37:12: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:37:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010726/SRX5010726.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling