Job ID = 12265541 SRX = SRX5010725 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:43 113325609 reads; of these: 113325609 (100.00%) were unpaired; of these: 2701934 (2.38%) aligned 0 times 88874996 (78.42%) aligned exactly 1 time 21748679 (19.19%) aligned >1 times 97.62% overall alignment rate Time searching: 00:27:43 Overall time: 00:27:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 70115595 / 110623675 = 0.6338 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:17:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:17:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:17:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:17:19: 1000000 INFO @ Sat, 03 Apr 2021 08:17:27: 2000000 INFO @ Sat, 03 Apr 2021 08:17:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:17:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:17:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:17:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:17:42: 4000000 INFO @ Sat, 03 Apr 2021 08:17:50: 1000000 INFO @ Sat, 03 Apr 2021 08:17:51: 5000000 INFO @ Sat, 03 Apr 2021 08:17:57: 2000000 INFO @ Sat, 03 Apr 2021 08:18:00: 6000000 INFO @ Sat, 03 Apr 2021 08:18:04: 3000000 INFO @ Sat, 03 Apr 2021 08:18:08: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:18:11: 4000000 INFO @ Sat, 03 Apr 2021 08:18:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:18:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:18:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:18:15: 8000000 INFO @ Sat, 03 Apr 2021 08:18:18: 5000000 INFO @ Sat, 03 Apr 2021 08:18:19: 1000000 INFO @ Sat, 03 Apr 2021 08:18:22: 9000000 INFO @ Sat, 03 Apr 2021 08:18:25: 6000000 INFO @ Sat, 03 Apr 2021 08:18:27: 2000000 INFO @ Sat, 03 Apr 2021 08:18:30: 10000000 INFO @ Sat, 03 Apr 2021 08:18:33: 7000000 INFO @ Sat, 03 Apr 2021 08:18:34: 3000000 INFO @ Sat, 03 Apr 2021 08:18:37: 11000000 INFO @ Sat, 03 Apr 2021 08:18:40: 8000000 INFO @ Sat, 03 Apr 2021 08:18:41: 4000000 INFO @ Sat, 03 Apr 2021 08:18:44: 12000000 INFO @ Sat, 03 Apr 2021 08:18:47: 9000000 INFO @ Sat, 03 Apr 2021 08:18:48: 5000000 INFO @ Sat, 03 Apr 2021 08:18:52: 13000000 INFO @ Sat, 03 Apr 2021 08:18:54: 10000000 INFO @ Sat, 03 Apr 2021 08:18:55: 6000000 INFO @ Sat, 03 Apr 2021 08:18:59: 14000000 INFO @ Sat, 03 Apr 2021 08:19:01: 11000000 INFO @ Sat, 03 Apr 2021 08:19:02: 7000000 INFO @ Sat, 03 Apr 2021 08:19:07: 15000000 INFO @ Sat, 03 Apr 2021 08:19:08: 12000000 INFO @ Sat, 03 Apr 2021 08:19:10: 8000000 INFO @ Sat, 03 Apr 2021 08:19:13: 16000000 INFO @ Sat, 03 Apr 2021 08:19:15: 13000000 INFO @ Sat, 03 Apr 2021 08:19:17: 9000000 INFO @ Sat, 03 Apr 2021 08:19:21: 17000000 INFO @ Sat, 03 Apr 2021 08:19:22: 14000000 INFO @ Sat, 03 Apr 2021 08:19:24: 10000000 INFO @ Sat, 03 Apr 2021 08:19:28: 18000000 INFO @ Sat, 03 Apr 2021 08:19:30: 15000000 INFO @ Sat, 03 Apr 2021 08:19:32: 11000000 INFO @ Sat, 03 Apr 2021 08:19:35: 19000000 INFO @ Sat, 03 Apr 2021 08:19:37: 16000000 INFO @ Sat, 03 Apr 2021 08:19:39: 12000000 INFO @ Sat, 03 Apr 2021 08:19:41: 20000000 INFO @ Sat, 03 Apr 2021 08:19:44: 17000000 INFO @ Sat, 03 Apr 2021 08:19:46: 13000000 INFO @ Sat, 03 Apr 2021 08:19:48: 21000000 INFO @ Sat, 03 Apr 2021 08:19:50: 18000000 INFO @ Sat, 03 Apr 2021 08:19:54: 14000000 INFO @ Sat, 03 Apr 2021 08:19:54: 22000000 INFO @ Sat, 03 Apr 2021 08:19:57: 19000000 INFO @ Sat, 03 Apr 2021 08:20:01: 23000000 INFO @ Sat, 03 Apr 2021 08:20:02: 15000000 INFO @ Sat, 03 Apr 2021 08:20:04: 20000000 INFO @ Sat, 03 Apr 2021 08:20:08: 24000000 INFO @ Sat, 03 Apr 2021 08:20:09: 16000000 INFO @ Sat, 03 Apr 2021 08:20:12: 21000000 INFO @ Sat, 03 Apr 2021 08:20:15: 25000000 INFO @ Sat, 03 Apr 2021 08:20:15: 17000000 INFO @ Sat, 03 Apr 2021 08:20:20: 22000000 INFO @ Sat, 03 Apr 2021 08:20:21: 26000000 INFO @ Sat, 03 Apr 2021 08:20:22: 18000000 INFO @ Sat, 03 Apr 2021 08:20:28: 23000000 INFO @ Sat, 03 Apr 2021 08:20:28: 19000000 INFO @ Sat, 03 Apr 2021 08:20:28: 27000000 INFO @ Sat, 03 Apr 2021 08:20:34: 20000000 INFO @ Sat, 03 Apr 2021 08:20:35: 28000000 INFO @ Sat, 03 Apr 2021 08:20:36: 24000000 INFO @ Sat, 03 Apr 2021 08:20:41: 21000000 INFO @ Sat, 03 Apr 2021 08:20:44: 29000000 INFO @ Sat, 03 Apr 2021 08:20:44: 25000000 INFO @ Sat, 03 Apr 2021 08:20:48: 22000000 INFO @ Sat, 03 Apr 2021 08:20:51: 30000000 INFO @ Sat, 03 Apr 2021 08:20:51: 26000000 INFO @ Sat, 03 Apr 2021 08:20:54: 23000000 INFO @ Sat, 03 Apr 2021 08:20:59: 31000000 INFO @ Sat, 03 Apr 2021 08:20:59: 27000000 INFO @ Sat, 03 Apr 2021 08:21:01: 24000000 INFO @ Sat, 03 Apr 2021 08:21:06: 28000000 INFO @ Sat, 03 Apr 2021 08:21:07: 32000000 INFO @ Sat, 03 Apr 2021 08:21:07: 25000000 INFO @ Sat, 03 Apr 2021 08:21:14: 29000000 INFO @ Sat, 03 Apr 2021 08:21:14: 26000000 INFO @ Sat, 03 Apr 2021 08:21:16: 33000000 INFO @ Sat, 03 Apr 2021 08:21:20: 27000000 INFO @ Sat, 03 Apr 2021 08:21:21: 30000000 INFO @ Sat, 03 Apr 2021 08:21:23: 34000000 INFO @ Sat, 03 Apr 2021 08:21:27: 28000000 INFO @ Sat, 03 Apr 2021 08:21:29: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:21:31: 35000000 INFO @ Sat, 03 Apr 2021 08:21:33: 29000000 INFO @ Sat, 03 Apr 2021 08:21:36: 32000000 INFO @ Sat, 03 Apr 2021 08:21:38: 36000000 INFO @ Sat, 03 Apr 2021 08:21:39: 30000000 INFO @ Sat, 03 Apr 2021 08:21:45: 37000000 INFO @ Sat, 03 Apr 2021 08:21:45: 33000000 INFO @ Sat, 03 Apr 2021 08:21:46: 31000000 INFO @ Sat, 03 Apr 2021 08:21:52: 38000000 INFO @ Sat, 03 Apr 2021 08:21:53: 32000000 INFO @ Sat, 03 Apr 2021 08:21:53: 34000000 INFO @ Sat, 03 Apr 2021 08:21:59: 39000000 INFO @ Sat, 03 Apr 2021 08:22:01: 35000000 INFO @ Sat, 03 Apr 2021 08:22:01: 33000000 INFO @ Sat, 03 Apr 2021 08:22:07: 40000000 INFO @ Sat, 03 Apr 2021 08:22:08: 36000000 INFO @ Sat, 03 Apr 2021 08:22:09: 34000000 INFO @ Sat, 03 Apr 2021 08:22:11: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:22:11: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:22:11: #1 total tags in treatment: 40508080 INFO @ Sat, 03 Apr 2021 08:22:11: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:22:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:22:13: #1 tags after filtering in treatment: 40508043 INFO @ Sat, 03 Apr 2021 08:22:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:22:13: #1 finished! INFO @ Sat, 03 Apr 2021 08:22:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:22:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:22:15: 37000000 INFO @ Sat, 03 Apr 2021 08:22:15: #2 number of paired peaks: 23 WARNING @ Sat, 03 Apr 2021 08:22:15: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:22:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:22:17: 35000000 INFO @ Sat, 03 Apr 2021 08:22:22: 38000000 INFO @ Sat, 03 Apr 2021 08:22:25: 36000000 INFO @ Sat, 03 Apr 2021 08:22:30: 39000000 INFO @ Sat, 03 Apr 2021 08:22:32: 37000000 INFO @ Sat, 03 Apr 2021 08:22:38: 40000000 INFO @ Sat, 03 Apr 2021 08:22:38: 38000000 INFO @ Sat, 03 Apr 2021 08:22:42: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:22:42: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:22:42: #1 total tags in treatment: 40508080 INFO @ Sat, 03 Apr 2021 08:22:42: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:22:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:22:44: #1 tags after filtering in treatment: 40508043 INFO @ Sat, 03 Apr 2021 08:22:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:22:44: #1 finished! INFO @ Sat, 03 Apr 2021 08:22:44: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:22:45: 39000000 INFO @ Sat, 03 Apr 2021 08:22:46: #2 number of paired peaks: 23 WARNING @ Sat, 03 Apr 2021 08:22:46: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:22:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:22:52: 40000000 INFO @ Sat, 03 Apr 2021 08:22:55: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:22:55: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:22:55: #1 total tags in treatment: 40508080 INFO @ Sat, 03 Apr 2021 08:22:55: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:22:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:22:57: #1 tags after filtering in treatment: 40508043 INFO @ Sat, 03 Apr 2021 08:22:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:22:57: #1 finished! INFO @ Sat, 03 Apr 2021 08:22:57: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:22:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:22:59: #2 number of paired peaks: 23 WARNING @ Sat, 03 Apr 2021 08:22:59: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:22:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010725/SRX5010725.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。