Job ID = 12265519 SRX = SRX5010724 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:36 112702038 reads; of these: 112702038 (100.00%) were unpaired; of these: 3023862 (2.68%) aligned 0 times 91853070 (81.50%) aligned exactly 1 time 17825106 (15.82%) aligned >1 times 97.32% overall alignment rate Time searching: 00:26:36 Overall time: 00:26:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 71756118 / 109678176 = 0.6542 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:05:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:05:27: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:05:27: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:05:33: 1000000 INFO @ Sat, 03 Apr 2021 08:05:38: 2000000 INFO @ Sat, 03 Apr 2021 08:05:44: 3000000 INFO @ Sat, 03 Apr 2021 08:05:49: 4000000 INFO @ Sat, 03 Apr 2021 08:05:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:05:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:05:57: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:05:57: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:06:01: 6000000 INFO @ Sat, 03 Apr 2021 08:06:03: 1000000 INFO @ Sat, 03 Apr 2021 08:06:07: 7000000 INFO @ Sat, 03 Apr 2021 08:06:09: 2000000 INFO @ Sat, 03 Apr 2021 08:06:14: 8000000 INFO @ Sat, 03 Apr 2021 08:06:16: 3000000 INFO @ Sat, 03 Apr 2021 08:06:20: 9000000 INFO @ Sat, 03 Apr 2021 08:06:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:06:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:06:27: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:06:27: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:06:27: 10000000 INFO @ Sat, 03 Apr 2021 08:06:29: 5000000 INFO @ Sat, 03 Apr 2021 08:06:33: 1000000 INFO @ Sat, 03 Apr 2021 08:06:33: 11000000 INFO @ Sat, 03 Apr 2021 08:06:35: 6000000 INFO @ Sat, 03 Apr 2021 08:06:40: 2000000 INFO @ Sat, 03 Apr 2021 08:06:40: 12000000 INFO @ Sat, 03 Apr 2021 08:06:42: 7000000 INFO @ Sat, 03 Apr 2021 08:06:46: 3000000 INFO @ Sat, 03 Apr 2021 08:06:46: 13000000 INFO @ Sat, 03 Apr 2021 08:06:48: 8000000 INFO @ Sat, 03 Apr 2021 08:06:53: 4000000 INFO @ Sat, 03 Apr 2021 08:06:53: 14000000 INFO @ Sat, 03 Apr 2021 08:06:55: 9000000 INFO @ Sat, 03 Apr 2021 08:06:59: 5000000 INFO @ Sat, 03 Apr 2021 08:06:59: 15000000 INFO @ Sat, 03 Apr 2021 08:07:01: 10000000 INFO @ Sat, 03 Apr 2021 08:07:06: 6000000 INFO @ Sat, 03 Apr 2021 08:07:06: 16000000 INFO @ Sat, 03 Apr 2021 08:07:08: 11000000 INFO @ Sat, 03 Apr 2021 08:07:12: 17000000 INFO @ Sat, 03 Apr 2021 08:07:12: 7000000 INFO @ Sat, 03 Apr 2021 08:07:14: 12000000 INFO @ Sat, 03 Apr 2021 08:07:19: 18000000 INFO @ Sat, 03 Apr 2021 08:07:19: 8000000 INFO @ Sat, 03 Apr 2021 08:07:21: 13000000 INFO @ Sat, 03 Apr 2021 08:07:25: 19000000 INFO @ Sat, 03 Apr 2021 08:07:26: 9000000 INFO @ Sat, 03 Apr 2021 08:07:27: 14000000 INFO @ Sat, 03 Apr 2021 08:07:32: 20000000 INFO @ Sat, 03 Apr 2021 08:07:32: 10000000 INFO @ Sat, 03 Apr 2021 08:07:34: 15000000 INFO @ Sat, 03 Apr 2021 08:07:38: 21000000 INFO @ Sat, 03 Apr 2021 08:07:38: 11000000 INFO @ Sat, 03 Apr 2021 08:07:40: 16000000 INFO @ Sat, 03 Apr 2021 08:07:45: 22000000 INFO @ Sat, 03 Apr 2021 08:07:45: 12000000 INFO @ Sat, 03 Apr 2021 08:07:47: 17000000 INFO @ Sat, 03 Apr 2021 08:07:51: 23000000 INFO @ Sat, 03 Apr 2021 08:07:51: 13000000 INFO @ Sat, 03 Apr 2021 08:07:53: 18000000 INFO @ Sat, 03 Apr 2021 08:07:58: 24000000 INFO @ Sat, 03 Apr 2021 08:07:58: 14000000 INFO @ Sat, 03 Apr 2021 08:07:59: 19000000 INFO @ Sat, 03 Apr 2021 08:08:04: 25000000 INFO @ Sat, 03 Apr 2021 08:08:04: 15000000 INFO @ Sat, 03 Apr 2021 08:08:06: 20000000 INFO @ Sat, 03 Apr 2021 08:08:11: 16000000 INFO @ Sat, 03 Apr 2021 08:08:11: 26000000 INFO @ Sat, 03 Apr 2021 08:08:12: 21000000 INFO @ Sat, 03 Apr 2021 08:08:17: 17000000 INFO @ Sat, 03 Apr 2021 08:08:17: 27000000 INFO @ Sat, 03 Apr 2021 08:08:19: 22000000 INFO @ Sat, 03 Apr 2021 08:08:23: 18000000 INFO @ Sat, 03 Apr 2021 08:08:23: 28000000 INFO @ Sat, 03 Apr 2021 08:08:25: 23000000 INFO @ Sat, 03 Apr 2021 08:08:30: 19000000 INFO @ Sat, 03 Apr 2021 08:08:30: 29000000 INFO @ Sat, 03 Apr 2021 08:08:31: 24000000 INFO @ Sat, 03 Apr 2021 08:08:36: 20000000 INFO @ Sat, 03 Apr 2021 08:08:36: 30000000 INFO @ Sat, 03 Apr 2021 08:08:38: 25000000 INFO @ Sat, 03 Apr 2021 08:08:42: 21000000 INFO @ Sat, 03 Apr 2021 08:08:43: 31000000 INFO @ Sat, 03 Apr 2021 08:08:44: 26000000 INFO @ Sat, 03 Apr 2021 08:08:49: 22000000 INFO @ Sat, 03 Apr 2021 08:08:50: 32000000 INFO @ Sat, 03 Apr 2021 08:08:51: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:08:55: 23000000 INFO @ Sat, 03 Apr 2021 08:08:56: 33000000 INFO @ Sat, 03 Apr 2021 08:08:57: 28000000 INFO @ Sat, 03 Apr 2021 08:09:02: 24000000 INFO @ Sat, 03 Apr 2021 08:09:03: 34000000 INFO @ Sat, 03 Apr 2021 08:09:04: 29000000 INFO @ Sat, 03 Apr 2021 08:09:08: 25000000 INFO @ Sat, 03 Apr 2021 08:09:09: 35000000 INFO @ Sat, 03 Apr 2021 08:09:10: 30000000 INFO @ Sat, 03 Apr 2021 08:09:15: 26000000 INFO @ Sat, 03 Apr 2021 08:09:16: 36000000 INFO @ Sat, 03 Apr 2021 08:09:17: 31000000 INFO @ Sat, 03 Apr 2021 08:09:21: 27000000 INFO @ Sat, 03 Apr 2021 08:09:22: 37000000 INFO @ Sat, 03 Apr 2021 08:09:23: 32000000 INFO @ Sat, 03 Apr 2021 08:09:28: 28000000 INFO @ Sat, 03 Apr 2021 08:09:28: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:09:28: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:09:28: #1 total tags in treatment: 37922058 INFO @ Sat, 03 Apr 2021 08:09:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:09:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:09:29: #1 tags after filtering in treatment: 37922003 INFO @ Sat, 03 Apr 2021 08:09:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:09:29: #1 finished! INFO @ Sat, 03 Apr 2021 08:09:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:09:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:09:30: 33000000 INFO @ Sat, 03 Apr 2021 08:09:32: #2 number of paired peaks: 36 WARNING @ Sat, 03 Apr 2021 08:09:32: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:09:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:09:34: 29000000 INFO @ Sat, 03 Apr 2021 08:09:36: 34000000 INFO @ Sat, 03 Apr 2021 08:09:40: 30000000 INFO @ Sat, 03 Apr 2021 08:09:42: 35000000 INFO @ Sat, 03 Apr 2021 08:09:47: 31000000 INFO @ Sat, 03 Apr 2021 08:09:49: 36000000 INFO @ Sat, 03 Apr 2021 08:09:53: 32000000 INFO @ Sat, 03 Apr 2021 08:09:55: 37000000 INFO @ Sat, 03 Apr 2021 08:10:00: 33000000 INFO @ Sat, 03 Apr 2021 08:10:01: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:10:01: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:10:01: #1 total tags in treatment: 37922058 INFO @ Sat, 03 Apr 2021 08:10:01: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:10:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:10:02: #1 tags after filtering in treatment: 37922003 INFO @ Sat, 03 Apr 2021 08:10:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:10:02: #1 finished! INFO @ Sat, 03 Apr 2021 08:10:02: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:10:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:10:04: #2 number of paired peaks: 36 WARNING @ Sat, 03 Apr 2021 08:10:04: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:10:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:10:06: 34000000 INFO @ Sat, 03 Apr 2021 08:10:11: 35000000 INFO @ Sat, 03 Apr 2021 08:10:17: 36000000 INFO @ Sat, 03 Apr 2021 08:10:23: 37000000 INFO @ Sat, 03 Apr 2021 08:10:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:10:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:10:29: #1 total tags in treatment: 37922058 INFO @ Sat, 03 Apr 2021 08:10:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:10:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:10:30: #1 tags after filtering in treatment: 37922003 INFO @ Sat, 03 Apr 2021 08:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 08:10:30: #1 finished! INFO @ Sat, 03 Apr 2021 08:10:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:10:32: #2 number of paired peaks: 36 WARNING @ Sat, 03 Apr 2021 08:10:32: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:10:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX5010724/SRX5010724.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling