Job ID = 14170662 SRX = SRX5007622 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:22 4363461 reads; of these: 4363461 (100.00%) were unpaired; of these: 511590 (11.72%) aligned 0 times 3233064 (74.09%) aligned exactly 1 time 618807 (14.18%) aligned >1 times 88.28% overall alignment rate Time searching: 00:03:22 Overall time: 00:03:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 2414264 / 3851871 = 0.6268 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:57:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:57:12: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:57:22: 1000000 INFO @ Sat, 11 Dec 2021 07:57:27: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 07:57:27: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 07:57:27: #1 total tags in treatment: 1437607 INFO @ Sat, 11 Dec 2021 07:57:27: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:57:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:57:27: #1 tags after filtering in treatment: 1437282 INFO @ Sat, 11 Dec 2021 07:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:57:27: #1 finished! INFO @ Sat, 11 Dec 2021 07:57:27: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:57:27: #2 number of paired peaks: 1180 INFO @ Sat, 11 Dec 2021 07:57:27: start model_add_line... INFO @ Sat, 11 Dec 2021 07:57:27: start X-correlation... INFO @ Sat, 11 Dec 2021 07:57:27: end of X-cor INFO @ Sat, 11 Dec 2021 07:57:27: #2 finished! INFO @ Sat, 11 Dec 2021 07:57:27: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 07:57:27: #2 alternative fragment length(s) may be 168,592 bps INFO @ Sat, 11 Dec 2021 07:57:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05_model.r WARNING @ Sat, 11 Dec 2021 07:57:27: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:57:27: #2 You may need to consider one of the other alternative d(s): 168,592 WARNING @ Sat, 11 Dec 2021 07:57:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:57:27: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:57:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:57:31: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:57:33: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05_peaks.xls INFO @ Sat, 11 Dec 2021 07:57:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:57:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.05_summits.bed INFO @ Sat, 11 Dec 2021 07:57:33: Done! pass1 - making usageList (275 chroms): 1 millis pass2 - checking and writing primary data (669 records, 4 fields): 8 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:57:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:57:42: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:57:42: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:57:50: 1000000 INFO @ Sat, 11 Dec 2021 07:57:53: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 07:57:53: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 07:57:53: #1 total tags in treatment: 1437607 INFO @ Sat, 11 Dec 2021 07:57:53: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:57:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:57:53: #1 tags after filtering in treatment: 1437282 INFO @ Sat, 11 Dec 2021 07:57:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:57:53: #1 finished! INFO @ Sat, 11 Dec 2021 07:57:53: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:57:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:57:54: #2 number of paired peaks: 1180 INFO @ Sat, 11 Dec 2021 07:57:54: start model_add_line... INFO @ Sat, 11 Dec 2021 07:57:54: start X-correlation... INFO @ Sat, 11 Dec 2021 07:57:54: end of X-cor INFO @ Sat, 11 Dec 2021 07:57:54: #2 finished! INFO @ Sat, 11 Dec 2021 07:57:54: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 07:57:54: #2 alternative fragment length(s) may be 168,592 bps INFO @ Sat, 11 Dec 2021 07:57:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10_model.r WARNING @ Sat, 11 Dec 2021 07:57:54: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:57:54: #2 You may need to consider one of the other alternative d(s): 168,592 WARNING @ Sat, 11 Dec 2021 07:57:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:57:54: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:57:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:57:57: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:57:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10_peaks.xls INFO @ Sat, 11 Dec 2021 07:57:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:57:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.10_summits.bed INFO @ Sat, 11 Dec 2021 07:57:59: Done! pass1 - making usageList (192 chroms): 1 millis pass2 - checking and writing primary data (331 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:58:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:58:12: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:58:12: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:58:22: 1000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:58:27: #1 tag size is determined as 151 bps INFO @ Sat, 11 Dec 2021 07:58:27: #1 tag size = 151 INFO @ Sat, 11 Dec 2021 07:58:27: #1 total tags in treatment: 1437607 INFO @ Sat, 11 Dec 2021 07:58:27: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:58:27: #1 tags after filtering in treatment: 1437282 INFO @ Sat, 11 Dec 2021 07:58:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 07:58:27: #1 finished! INFO @ Sat, 11 Dec 2021 07:58:27: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:58:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:58:27: #2 number of paired peaks: 1180 INFO @ Sat, 11 Dec 2021 07:58:27: start model_add_line... INFO @ Sat, 11 Dec 2021 07:58:27: start X-correlation... INFO @ Sat, 11 Dec 2021 07:58:27: end of X-cor INFO @ Sat, 11 Dec 2021 07:58:27: #2 finished! INFO @ Sat, 11 Dec 2021 07:58:27: #2 predicted fragment length is 168 bps INFO @ Sat, 11 Dec 2021 07:58:27: #2 alternative fragment length(s) may be 168,592 bps INFO @ Sat, 11 Dec 2021 07:58:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20_model.r WARNING @ Sat, 11 Dec 2021 07:58:27: #2 Since the d (168) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:58:27: #2 You may need to consider one of the other alternative d(s): 168,592 WARNING @ Sat, 11 Dec 2021 07:58:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:58:27: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:58:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:58:31: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:58:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20_peaks.xls INFO @ Sat, 11 Dec 2021 07:58:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:58:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX5007622/SRX5007622.20_summits.bed INFO @ Sat, 11 Dec 2021 07:58:32: Done! pass1 - making usageList (89 chroms): 1 millis pass2 - checking and writing primary data (135 records, 4 fields): 3 millis CompletedMACS2peakCalling