Job ID = 6458227 SRX = SRX495784 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:17:19 prefetch.2.10.7: 1) Downloading 'SRR1199482'... 2020-06-21T12:17:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:21:23 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:21:23 prefetch.2.10.7: 1) 'SRR1199482' was downloaded successfully Read 28614306 spots for SRR1199482/SRR1199482.sra Written 28614306 spots for SRR1199482/SRR1199482.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:01 28614306 reads; of these: 28614306 (100.00%) were unpaired; of these: 9344630 (32.66%) aligned 0 times 15700045 (54.87%) aligned exactly 1 time 3569631 (12.47%) aligned >1 times 67.34% overall alignment rate Time searching: 00:06:01 Overall time: 00:06:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3460210 / 19269676 = 0.1796 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:33:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:33:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:33:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:33:46: 1000000 INFO @ Sun, 21 Jun 2020 21:33:51: 2000000 INFO @ Sun, 21 Jun 2020 21:33:57: 3000000 INFO @ Sun, 21 Jun 2020 21:34:02: 4000000 INFO @ Sun, 21 Jun 2020 21:34:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:34:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:34:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:34:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:34:14: 6000000 INFO @ Sun, 21 Jun 2020 21:34:15: 1000000 INFO @ Sun, 21 Jun 2020 21:34:19: 7000000 INFO @ Sun, 21 Jun 2020 21:34:20: 2000000 INFO @ Sun, 21 Jun 2020 21:34:25: 3000000 INFO @ Sun, 21 Jun 2020 21:34:25: 8000000 INFO @ Sun, 21 Jun 2020 21:34:30: 4000000 INFO @ Sun, 21 Jun 2020 21:34:31: 9000000 INFO @ Sun, 21 Jun 2020 21:34:35: 5000000 INFO @ Sun, 21 Jun 2020 21:34:37: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:34:40: 6000000 INFO @ Sun, 21 Jun 2020 21:34:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:34:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:34:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:34:42: 11000000 INFO @ Sun, 21 Jun 2020 21:34:45: 7000000 INFO @ Sun, 21 Jun 2020 21:34:45: 1000000 INFO @ Sun, 21 Jun 2020 21:34:48: 12000000 INFO @ Sun, 21 Jun 2020 21:34:50: 8000000 INFO @ Sun, 21 Jun 2020 21:34:50: 2000000 INFO @ Sun, 21 Jun 2020 21:34:54: 13000000 INFO @ Sun, 21 Jun 2020 21:34:55: 9000000 INFO @ Sun, 21 Jun 2020 21:34:55: 3000000 INFO @ Sun, 21 Jun 2020 21:35:00: 14000000 INFO @ Sun, 21 Jun 2020 21:35:00: 10000000 INFO @ Sun, 21 Jun 2020 21:35:01: 4000000 INFO @ Sun, 21 Jun 2020 21:35:05: 11000000 INFO @ Sun, 21 Jun 2020 21:35:05: 15000000 INFO @ Sun, 21 Jun 2020 21:35:06: 5000000 INFO @ Sun, 21 Jun 2020 21:35:10: 12000000 INFO @ Sun, 21 Jun 2020 21:35:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:35:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:35:10: #1 total tags in treatment: 15809466 INFO @ Sun, 21 Jun 2020 21:35:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:11: #1 tags after filtering in treatment: 15809462 INFO @ Sun, 21 Jun 2020 21:35:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:11: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:11: 6000000 INFO @ Sun, 21 Jun 2020 21:35:12: #2 number of paired peaks: 78 WARNING @ Sun, 21 Jun 2020 21:35:12: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:35:15: 13000000 INFO @ Sun, 21 Jun 2020 21:35:16: 7000000 INFO @ Sun, 21 Jun 2020 21:35:20: 14000000 INFO @ Sun, 21 Jun 2020 21:35:21: 8000000 INFO @ Sun, 21 Jun 2020 21:35:24: 15000000 INFO @ Sun, 21 Jun 2020 21:35:26: 9000000 INFO @ Sun, 21 Jun 2020 21:35:29: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:35:29: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:35:29: #1 total tags in treatment: 15809466 INFO @ Sun, 21 Jun 2020 21:35:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:35:29: #1 tags after filtering in treatment: 15809462 INFO @ Sun, 21 Jun 2020 21:35:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:35:29: #1 finished! INFO @ Sun, 21 Jun 2020 21:35:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:35:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:35:30: #2 number of paired peaks: 78 WARNING @ Sun, 21 Jun 2020 21:35:30: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:35:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:35:31: 10000000 INFO @ Sun, 21 Jun 2020 21:35:36: 11000000 INFO @ Sun, 21 Jun 2020 21:35:40: 12000000 INFO @ Sun, 21 Jun 2020 21:35:45: 13000000 INFO @ Sun, 21 Jun 2020 21:35:50: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:35:55: 15000000 INFO @ Sun, 21 Jun 2020 21:35:59: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:35:59: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:35:59: #1 total tags in treatment: 15809466 INFO @ Sun, 21 Jun 2020 21:35:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:35:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:36:00: #1 tags after filtering in treatment: 15809462 INFO @ Sun, 21 Jun 2020 21:36:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:36:00: #1 finished! INFO @ Sun, 21 Jun 2020 21:36:00: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:36:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:36:01: #2 number of paired peaks: 78 WARNING @ Sun, 21 Jun 2020 21:36:01: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:36:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495784/SRX495784.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。