Job ID = 6458225 SRX = SRX495782 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:29:49 prefetch.2.10.7: 1) Downloading 'SRR1199480'... 2020-06-21T12:29:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:35:03 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:35:03 prefetch.2.10.7: 1) 'SRR1199480' was downloaded successfully Read 33349222 spots for SRR1199480/SRR1199480.sra Written 33349222 spots for SRR1199480/SRR1199480.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:43 33349222 reads; of these: 33349222 (100.00%) were unpaired; of these: 5059642 (15.17%) aligned 0 times 22969549 (68.88%) aligned exactly 1 time 5320031 (15.95%) aligned >1 times 84.83% overall alignment rate Time searching: 00:09:43 Overall time: 00:09:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6269065 / 28289580 = 0.2216 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:38: 1000000 INFO @ Sun, 21 Jun 2020 21:53:43: 2000000 INFO @ Sun, 21 Jun 2020 21:53:48: 3000000 INFO @ Sun, 21 Jun 2020 21:53:53: 4000000 INFO @ Sun, 21 Jun 2020 21:53:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:54:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:54:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:54:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:54:03: 6000000 INFO @ Sun, 21 Jun 2020 21:54:09: 7000000 INFO @ Sun, 21 Jun 2020 21:54:09: 1000000 INFO @ Sun, 21 Jun 2020 21:54:14: 8000000 INFO @ Sun, 21 Jun 2020 21:54:14: 2000000 INFO @ Sun, 21 Jun 2020 21:54:19: 9000000 INFO @ Sun, 21 Jun 2020 21:54:20: 3000000 INFO @ Sun, 21 Jun 2020 21:54:25: 10000000 INFO @ Sun, 21 Jun 2020 21:54:26: 4000000 INFO @ Sun, 21 Jun 2020 21:54:30: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:54:32: 5000000 INFO @ Sun, 21 Jun 2020 21:54:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:54:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:54:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:54:35: 12000000 INFO @ Sun, 21 Jun 2020 21:54:38: 6000000 INFO @ Sun, 21 Jun 2020 21:54:39: 1000000 INFO @ Sun, 21 Jun 2020 21:54:41: 13000000 INFO @ Sun, 21 Jun 2020 21:54:44: 7000000 INFO @ Sun, 21 Jun 2020 21:54:44: 2000000 INFO @ Sun, 21 Jun 2020 21:54:46: 14000000 INFO @ Sun, 21 Jun 2020 21:54:50: 8000000 INFO @ Sun, 21 Jun 2020 21:54:50: 3000000 INFO @ Sun, 21 Jun 2020 21:54:52: 15000000 INFO @ Sun, 21 Jun 2020 21:54:55: 4000000 INFO @ Sun, 21 Jun 2020 21:54:55: 9000000 INFO @ Sun, 21 Jun 2020 21:54:57: 16000000 INFO @ Sun, 21 Jun 2020 21:55:01: 5000000 INFO @ Sun, 21 Jun 2020 21:55:01: 10000000 INFO @ Sun, 21 Jun 2020 21:55:03: 17000000 INFO @ Sun, 21 Jun 2020 21:55:06: 6000000 INFO @ Sun, 21 Jun 2020 21:55:07: 11000000 INFO @ Sun, 21 Jun 2020 21:55:09: 18000000 INFO @ Sun, 21 Jun 2020 21:55:12: 7000000 INFO @ Sun, 21 Jun 2020 21:55:13: 12000000 INFO @ Sun, 21 Jun 2020 21:55:15: 19000000 INFO @ Sun, 21 Jun 2020 21:55:18: 8000000 INFO @ Sun, 21 Jun 2020 21:55:19: 13000000 INFO @ Sun, 21 Jun 2020 21:55:21: 20000000 INFO @ Sun, 21 Jun 2020 21:55:23: 9000000 INFO @ Sun, 21 Jun 2020 21:55:26: 14000000 INFO @ Sun, 21 Jun 2020 21:55:27: 21000000 INFO @ Sun, 21 Jun 2020 21:55:29: 10000000 INFO @ Sun, 21 Jun 2020 21:55:32: 15000000 INFO @ Sun, 21 Jun 2020 21:55:33: 22000000 INFO @ Sun, 21 Jun 2020 21:55:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:33: #1 total tags in treatment: 22020515 INFO @ Sun, 21 Jun 2020 21:55:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:34: #1 tags after filtering in treatment: 22020513 INFO @ Sun, 21 Jun 2020 21:55:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:34: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:35: 11000000 INFO @ Sun, 21 Jun 2020 21:55:35: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 21:55:35: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:38: 16000000 INFO @ Sun, 21 Jun 2020 21:55:40: 12000000 INFO @ Sun, 21 Jun 2020 21:55:45: 17000000 INFO @ Sun, 21 Jun 2020 21:55:46: 13000000 INFO @ Sun, 21 Jun 2020 21:55:52: 18000000 INFO @ Sun, 21 Jun 2020 21:55:52: 14000000 INFO @ Sun, 21 Jun 2020 21:55:57: 19000000 INFO @ Sun, 21 Jun 2020 21:55:58: 15000000 INFO @ Sun, 21 Jun 2020 21:56:03: 16000000 INFO @ Sun, 21 Jun 2020 21:56:04: 20000000 INFO @ Sun, 21 Jun 2020 21:56:09: 17000000 INFO @ Sun, 21 Jun 2020 21:56:10: 21000000 INFO @ Sun, 21 Jun 2020 21:56:15: 18000000 INFO @ Sun, 21 Jun 2020 21:56:16: 22000000 INFO @ Sun, 21 Jun 2020 21:56:17: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:17: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:17: #1 total tags in treatment: 22020515 INFO @ Sun, 21 Jun 2020 21:56:17: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:17: #1 tags after filtering in treatment: 22020513 INFO @ Sun, 21 Jun 2020 21:56:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:17: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:19: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 21:56:19: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:56:21: 19000000 INFO @ Sun, 21 Jun 2020 21:56:27: 20000000 INFO @ Sun, 21 Jun 2020 21:56:33: 21000000 INFO @ Sun, 21 Jun 2020 21:56:38: 22000000 INFO @ Sun, 21 Jun 2020 21:56:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:39: #1 total tags in treatment: 22020515 INFO @ Sun, 21 Jun 2020 21:56:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:39: #1 tags after filtering in treatment: 22020513 INFO @ Sun, 21 Jun 2020 21:56:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:39: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:39: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:41: #2 number of paired peaks: 32 WARNING @ Sun, 21 Jun 2020 21:56:41: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495782/SRX495782.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。