Job ID = 6458224 SRX = SRX495781 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:29:04 prefetch.2.10.7: 1) Downloading 'SRR1199479'... 2020-06-21T12:29:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:34:15 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:34:15 prefetch.2.10.7: 1) 'SRR1199479' was downloaded successfully Read 31886596 spots for SRR1199479/SRR1199479.sra Written 31886596 spots for SRR1199479/SRR1199479.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 31886596 reads; of these: 31886596 (100.00%) were unpaired; of these: 2902483 (9.10%) aligned 0 times 22960063 (72.01%) aligned exactly 1 time 6024050 (18.89%) aligned >1 times 90.90% overall alignment rate Time searching: 00:08:14 Overall time: 00:08:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7862938 / 28984113 = 0.2713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:50:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:50:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:50:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:50:47: 1000000 INFO @ Sun, 21 Jun 2020 21:50:53: 2000000 INFO @ Sun, 21 Jun 2020 21:50:59: 3000000 INFO @ Sun, 21 Jun 2020 21:51:04: 4000000 INFO @ Sun, 21 Jun 2020 21:51:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:51:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:51:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:51:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:51:16: 6000000 INFO @ Sun, 21 Jun 2020 21:51:18: 1000000 INFO @ Sun, 21 Jun 2020 21:51:22: 7000000 INFO @ Sun, 21 Jun 2020 21:51:24: 2000000 INFO @ Sun, 21 Jun 2020 21:51:28: 8000000 INFO @ Sun, 21 Jun 2020 21:51:31: 3000000 INFO @ Sun, 21 Jun 2020 21:51:34: 9000000 INFO @ Sun, 21 Jun 2020 21:51:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:51:40: 10000000 INFO @ Sun, 21 Jun 2020 21:51:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:51:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:51:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:51:43: 5000000 INFO @ Sun, 21 Jun 2020 21:51:47: 11000000 INFO @ Sun, 21 Jun 2020 21:51:48: 1000000 INFO @ Sun, 21 Jun 2020 21:51:49: 6000000 INFO @ Sun, 21 Jun 2020 21:51:53: 12000000 INFO @ Sun, 21 Jun 2020 21:51:54: 2000000 INFO @ Sun, 21 Jun 2020 21:51:55: 7000000 INFO @ Sun, 21 Jun 2020 21:51:59: 13000000 INFO @ Sun, 21 Jun 2020 21:52:01: 3000000 INFO @ Sun, 21 Jun 2020 21:52:02: 8000000 INFO @ Sun, 21 Jun 2020 21:52:05: 14000000 INFO @ Sun, 21 Jun 2020 21:52:07: 4000000 INFO @ Sun, 21 Jun 2020 21:52:08: 9000000 INFO @ Sun, 21 Jun 2020 21:52:12: 15000000 INFO @ Sun, 21 Jun 2020 21:52:13: 5000000 INFO @ Sun, 21 Jun 2020 21:52:14: 10000000 INFO @ Sun, 21 Jun 2020 21:52:18: 16000000 INFO @ Sun, 21 Jun 2020 21:52:19: 6000000 INFO @ Sun, 21 Jun 2020 21:52:20: 11000000 INFO @ Sun, 21 Jun 2020 21:52:25: 17000000 INFO @ Sun, 21 Jun 2020 21:52:26: 7000000 INFO @ Sun, 21 Jun 2020 21:52:27: 12000000 INFO @ Sun, 21 Jun 2020 21:52:31: 18000000 INFO @ Sun, 21 Jun 2020 21:52:32: 8000000 INFO @ Sun, 21 Jun 2020 21:52:33: 13000000 INFO @ Sun, 21 Jun 2020 21:52:37: 19000000 INFO @ Sun, 21 Jun 2020 21:52:38: 9000000 INFO @ Sun, 21 Jun 2020 21:52:39: 14000000 INFO @ Sun, 21 Jun 2020 21:52:44: 20000000 INFO @ Sun, 21 Jun 2020 21:52:44: 10000000 INFO @ Sun, 21 Jun 2020 21:52:46: 15000000 INFO @ Sun, 21 Jun 2020 21:52:50: 21000000 INFO @ Sun, 21 Jun 2020 21:52:51: 11000000 INFO @ Sun, 21 Jun 2020 21:52:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:52:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:52:51: #1 total tags in treatment: 21121175 INFO @ Sun, 21 Jun 2020 21:52:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:52:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:52:52: #1 tags after filtering in treatment: 21121119 INFO @ Sun, 21 Jun 2020 21:52:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:52:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:52:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:52:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:52:52: 16000000 INFO @ Sun, 21 Jun 2020 21:52:53: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 21:52:53: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:52:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:52:57: 12000000 INFO @ Sun, 21 Jun 2020 21:52:59: 17000000 INFO @ Sun, 21 Jun 2020 21:53:03: 13000000 INFO @ Sun, 21 Jun 2020 21:53:06: 18000000 INFO @ Sun, 21 Jun 2020 21:53:09: 14000000 INFO @ Sun, 21 Jun 2020 21:53:12: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:53:15: 15000000 INFO @ Sun, 21 Jun 2020 21:53:18: 20000000 INFO @ Sun, 21 Jun 2020 21:53:22: 16000000 INFO @ Sun, 21 Jun 2020 21:53:25: 21000000 INFO @ Sun, 21 Jun 2020 21:53:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:53:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:53:26: #1 total tags in treatment: 21121175 INFO @ Sun, 21 Jun 2020 21:53:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:53:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:53:26: #1 tags after filtering in treatment: 21121119 INFO @ Sun, 21 Jun 2020 21:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:53:26: #1 finished! INFO @ Sun, 21 Jun 2020 21:53:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:53:28: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 21:53:28: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:53:28: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 21:53:28: 17000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:53:34: 18000000 INFO @ Sun, 21 Jun 2020 21:53:39: 19000000 INFO @ Sun, 21 Jun 2020 21:53:45: 20000000 INFO @ Sun, 21 Jun 2020 21:53:50: 21000000 INFO @ Sun, 21 Jun 2020 21:53:51: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:53:51: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:53:51: #1 total tags in treatment: 21121175 INFO @ Sun, 21 Jun 2020 21:53:51: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:53:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:53:52: #1 tags after filtering in treatment: 21121119 INFO @ Sun, 21 Jun 2020 21:53:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:53:52: #1 finished! INFO @ Sun, 21 Jun 2020 21:53:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:53:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:53:53: #2 number of paired peaks: 28 WARNING @ Sun, 21 Jun 2020 21:53:53: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:53:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495781/SRX495781.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。