Job ID = 6458223 SRX = SRX495780 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:22:49 prefetch.2.10.7: 1) Downloading 'SRR1199478'... 2020-06-21T12:22:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:27:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:27:14 prefetch.2.10.7: 1) 'SRR1199478' was downloaded successfully Read 32203362 spots for SRR1199478/SRR1199478.sra Written 32203362 spots for SRR1199478/SRR1199478.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 32203362 reads; of these: 32203362 (100.00%) were unpaired; of these: 3233957 (10.04%) aligned 0 times 22565229 (70.07%) aligned exactly 1 time 6404176 (19.89%) aligned >1 times 89.96% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7795061 / 28969405 = 0.2691 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:43:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:43:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:43:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:43:56: 1000000 INFO @ Sun, 21 Jun 2020 21:44:02: 2000000 INFO @ Sun, 21 Jun 2020 21:44:08: 3000000 INFO @ Sun, 21 Jun 2020 21:44:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:44:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:44:19: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:44:19: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:44:20: 5000000 INFO @ Sun, 21 Jun 2020 21:44:26: 1000000 INFO @ Sun, 21 Jun 2020 21:44:26: 6000000 INFO @ Sun, 21 Jun 2020 21:44:32: 2000000 INFO @ Sun, 21 Jun 2020 21:44:32: 7000000 INFO @ Sun, 21 Jun 2020 21:44:38: 3000000 INFO @ Sun, 21 Jun 2020 21:44:38: 8000000 INFO @ Sun, 21 Jun 2020 21:44:44: 4000000 INFO @ Sun, 21 Jun 2020 21:44:45: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:44:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:44:49: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:44:49: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:44:51: 5000000 INFO @ Sun, 21 Jun 2020 21:44:51: 10000000 INFO @ Sun, 21 Jun 2020 21:44:54: 1000000 INFO @ Sun, 21 Jun 2020 21:44:57: 11000000 INFO @ Sun, 21 Jun 2020 21:44:57: 6000000 INFO @ Sun, 21 Jun 2020 21:45:00: 2000000 INFO @ Sun, 21 Jun 2020 21:45:03: 12000000 INFO @ Sun, 21 Jun 2020 21:45:03: 7000000 INFO @ Sun, 21 Jun 2020 21:45:05: 3000000 INFO @ Sun, 21 Jun 2020 21:45:09: 13000000 INFO @ Sun, 21 Jun 2020 21:45:09: 8000000 INFO @ Sun, 21 Jun 2020 21:45:11: 4000000 INFO @ Sun, 21 Jun 2020 21:45:15: 14000000 INFO @ Sun, 21 Jun 2020 21:45:15: 9000000 INFO @ Sun, 21 Jun 2020 21:45:16: 5000000 INFO @ Sun, 21 Jun 2020 21:45:21: 15000000 INFO @ Sun, 21 Jun 2020 21:45:21: 6000000 INFO @ Sun, 21 Jun 2020 21:45:21: 10000000 INFO @ Sun, 21 Jun 2020 21:45:27: 7000000 INFO @ Sun, 21 Jun 2020 21:45:27: 16000000 INFO @ Sun, 21 Jun 2020 21:45:27: 11000000 INFO @ Sun, 21 Jun 2020 21:45:32: 8000000 INFO @ Sun, 21 Jun 2020 21:45:33: 17000000 INFO @ Sun, 21 Jun 2020 21:45:34: 12000000 INFO @ Sun, 21 Jun 2020 21:45:37: 9000000 INFO @ Sun, 21 Jun 2020 21:45:39: 18000000 INFO @ Sun, 21 Jun 2020 21:45:40: 13000000 INFO @ Sun, 21 Jun 2020 21:45:42: 10000000 INFO @ Sun, 21 Jun 2020 21:45:45: 19000000 INFO @ Sun, 21 Jun 2020 21:45:46: 14000000 INFO @ Sun, 21 Jun 2020 21:45:48: 11000000 INFO @ Sun, 21 Jun 2020 21:45:51: 20000000 INFO @ Sun, 21 Jun 2020 21:45:52: 15000000 INFO @ Sun, 21 Jun 2020 21:45:53: 12000000 INFO @ Sun, 21 Jun 2020 21:45:57: 21000000 INFO @ Sun, 21 Jun 2020 21:45:58: 16000000 INFO @ Sun, 21 Jun 2020 21:45:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:45:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:45:58: #1 total tags in treatment: 21174344 INFO @ Sun, 21 Jun 2020 21:45:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:45:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:45:58: 13000000 INFO @ Sun, 21 Jun 2020 21:45:59: #1 tags after filtering in treatment: 21174285 INFO @ Sun, 21 Jun 2020 21:45:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:45:59: #1 finished! INFO @ Sun, 21 Jun 2020 21:45:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:45:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:00: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 21:46:00: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:46:03: 14000000 INFO @ Sun, 21 Jun 2020 21:46:04: 17000000 INFO @ Sun, 21 Jun 2020 21:46:08: 15000000 INFO @ Sun, 21 Jun 2020 21:46:10: 18000000 INFO @ Sun, 21 Jun 2020 21:46:14: 16000000 INFO @ Sun, 21 Jun 2020 21:46:16: 19000000 INFO @ Sun, 21 Jun 2020 21:46:19: 17000000 INFO @ Sun, 21 Jun 2020 21:46:22: 20000000 INFO @ Sun, 21 Jun 2020 21:46:24: 18000000 INFO @ Sun, 21 Jun 2020 21:46:29: 21000000 INFO @ Sun, 21 Jun 2020 21:46:30: 19000000 INFO @ Sun, 21 Jun 2020 21:46:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:46:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:46:30: #1 total tags in treatment: 21174344 INFO @ Sun, 21 Jun 2020 21:46:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:46:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:46:30: #1 tags after filtering in treatment: 21174285 INFO @ Sun, 21 Jun 2020 21:46:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:46:30: #1 finished! INFO @ Sun, 21 Jun 2020 21:46:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:46:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:32: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 21:46:32: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:46:35: 20000000 INFO @ Sun, 21 Jun 2020 21:46:40: 21000000 INFO @ Sun, 21 Jun 2020 21:46:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:46:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:46:41: #1 total tags in treatment: 21174344 INFO @ Sun, 21 Jun 2020 21:46:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:46:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:46:41: #1 tags after filtering in treatment: 21174285 INFO @ Sun, 21 Jun 2020 21:46:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:46:41: #1 finished! INFO @ Sun, 21 Jun 2020 21:46:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:46:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:43: #2 number of paired peaks: 64 WARNING @ Sun, 21 Jun 2020 21:46:43: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495780/SRX495780.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。