Job ID = 6458222 SRX = SRX495779 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:12:34 prefetch.2.10.7: 1) Downloading 'SRR1199477'... 2020-06-21T12:12:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:20:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:20:37 prefetch.2.10.7: 1) 'SRR1199477' was downloaded successfully Read 56147132 spots for SRR1199477/SRR1199477.sra Written 56147132 spots for SRR1199477/SRR1199477.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:05 56147132 reads; of these: 56147132 (100.00%) were unpaired; of these: 12280180 (21.87%) aligned 0 times 36903929 (65.73%) aligned exactly 1 time 6963023 (12.40%) aligned >1 times 78.13% overall alignment rate Time searching: 00:12:05 Overall time: 00:12:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17121210 / 43866952 = 0.3903 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:46:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:46:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:46:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:46:47: 1000000 INFO @ Sun, 21 Jun 2020 21:46:55: 2000000 INFO @ Sun, 21 Jun 2020 21:47:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:09: 4000000 INFO @ Sun, 21 Jun 2020 21:47:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:16: 5000000 INFO @ Sun, 21 Jun 2020 21:47:18: 1000000 INFO @ Sun, 21 Jun 2020 21:47:24: 6000000 INFO @ Sun, 21 Jun 2020 21:47:26: 2000000 INFO @ Sun, 21 Jun 2020 21:47:32: 7000000 INFO @ Sun, 21 Jun 2020 21:47:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:39: 8000000 INFO @ Sun, 21 Jun 2020 21:47:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:41: 4000000 INFO @ Sun, 21 Jun 2020 21:47:47: 9000000 INFO @ Sun, 21 Jun 2020 21:47:48: 1000000 INFO @ Sun, 21 Jun 2020 21:47:49: 5000000 INFO @ Sun, 21 Jun 2020 21:47:54: 10000000 INFO @ Sun, 21 Jun 2020 21:47:56: 2000000 INFO @ Sun, 21 Jun 2020 21:47:56: 6000000 INFO @ Sun, 21 Jun 2020 21:48:02: 11000000 INFO @ Sun, 21 Jun 2020 21:48:03: 3000000 INFO @ Sun, 21 Jun 2020 21:48:04: 7000000 INFO @ Sun, 21 Jun 2020 21:48:10: 12000000 INFO @ Sun, 21 Jun 2020 21:48:11: 4000000 INFO @ Sun, 21 Jun 2020 21:48:11: 8000000 INFO @ Sun, 21 Jun 2020 21:48:17: 13000000 INFO @ Sun, 21 Jun 2020 21:48:19: 5000000 INFO @ Sun, 21 Jun 2020 21:48:19: 9000000 INFO @ Sun, 21 Jun 2020 21:48:25: 14000000 INFO @ Sun, 21 Jun 2020 21:48:26: 6000000 INFO @ Sun, 21 Jun 2020 21:48:26: 10000000 INFO @ Sun, 21 Jun 2020 21:48:32: 15000000 INFO @ Sun, 21 Jun 2020 21:48:33: 7000000 INFO @ Sun, 21 Jun 2020 21:48:34: 11000000 INFO @ Sun, 21 Jun 2020 21:48:40: 16000000 INFO @ Sun, 21 Jun 2020 21:48:41: 8000000 INFO @ Sun, 21 Jun 2020 21:48:41: 12000000 INFO @ Sun, 21 Jun 2020 21:48:47: 17000000 INFO @ Sun, 21 Jun 2020 21:48:48: 9000000 INFO @ Sun, 21 Jun 2020 21:48:49: 13000000 INFO @ Sun, 21 Jun 2020 21:48:54: 18000000 INFO @ Sun, 21 Jun 2020 21:48:55: 10000000 INFO @ Sun, 21 Jun 2020 21:48:56: 14000000 INFO @ Sun, 21 Jun 2020 21:49:01: 19000000 INFO @ Sun, 21 Jun 2020 21:49:02: 11000000 INFO @ Sun, 21 Jun 2020 21:49:03: 15000000 INFO @ Sun, 21 Jun 2020 21:49:09: 20000000 INFO @ Sun, 21 Jun 2020 21:49:09: 12000000 INFO @ Sun, 21 Jun 2020 21:49:10: 16000000 INFO @ Sun, 21 Jun 2020 21:49:16: 21000000 INFO @ Sun, 21 Jun 2020 21:49:17: 13000000 INFO @ Sun, 21 Jun 2020 21:49:17: 17000000 INFO @ Sun, 21 Jun 2020 21:49:24: 22000000 INFO @ Sun, 21 Jun 2020 21:49:24: 14000000 INFO @ Sun, 21 Jun 2020 21:49:25: 18000000 INFO @ Sun, 21 Jun 2020 21:49:31: 23000000 INFO @ Sun, 21 Jun 2020 21:49:31: 15000000 INFO @ Sun, 21 Jun 2020 21:49:32: 19000000 INFO @ Sun, 21 Jun 2020 21:49:38: 24000000 INFO @ Sun, 21 Jun 2020 21:49:38: 16000000 INFO @ Sun, 21 Jun 2020 21:49:39: 20000000 INFO @ Sun, 21 Jun 2020 21:49:45: 25000000 INFO @ Sun, 21 Jun 2020 21:49:45: 17000000 INFO @ Sun, 21 Jun 2020 21:49:46: 21000000 INFO @ Sun, 21 Jun 2020 21:49:52: 26000000 INFO @ Sun, 21 Jun 2020 21:49:52: 18000000 INFO @ Sun, 21 Jun 2020 21:49:54: 22000000 INFO @ Sun, 21 Jun 2020 21:49:58: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:49:58: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:49:58: #1 total tags in treatment: 26745742 INFO @ Sun, 21 Jun 2020 21:49:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:49:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:49:59: #1 tags after filtering in treatment: 26745695 INFO @ Sun, 21 Jun 2020 21:49:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:49:59: #1 finished! INFO @ Sun, 21 Jun 2020 21:49:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:49:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:49:59: 19000000 INFO @ Sun, 21 Jun 2020 21:50:01: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:50:01: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:50:01: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:50:06: 20000000 INFO @ Sun, 21 Jun 2020 21:50:08: 24000000 INFO @ Sun, 21 Jun 2020 21:50:13: 21000000 INFO @ Sun, 21 Jun 2020 21:50:15: 25000000 INFO @ Sun, 21 Jun 2020 21:50:21: 22000000 INFO @ Sun, 21 Jun 2020 21:50:22: 26000000 INFO @ Sun, 21 Jun 2020 21:50:27: 23000000 INFO @ Sun, 21 Jun 2020 21:50:28: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:50:28: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:50:28: #1 total tags in treatment: 26745742 INFO @ Sun, 21 Jun 2020 21:50:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:29: #1 tags after filtering in treatment: 26745695 INFO @ Sun, 21 Jun 2020 21:50:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:29: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:30: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:50:30: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:50:34: 24000000 INFO @ Sun, 21 Jun 2020 21:50:40: 25000000 INFO @ Sun, 21 Jun 2020 21:50:46: 26000000 INFO @ Sun, 21 Jun 2020 21:50:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:50:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:50:52: #1 total tags in treatment: 26745742 INFO @ Sun, 21 Jun 2020 21:50:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:53: #1 tags after filtering in treatment: 26745695 INFO @ Sun, 21 Jun 2020 21:50:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:53: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:55: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:50:55: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495779/SRX495779.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。