Job ID = 6458219 SRX = SRX495776 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:16:34 prefetch.2.10.7: 1) Downloading 'SRR1199474'... 2020-06-21T12:16:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:19:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:19:15 prefetch.2.10.7: 'SRR1199474' is valid 2020-06-21T12:19:15 prefetch.2.10.7: 1) 'SRR1199474' was downloaded successfully Read 19927623 spots for SRR1199474/SRR1199474.sra Written 19927623 spots for SRR1199474/SRR1199474.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 336361 (1.69%) aligned 0 times 15093153 (75.74%) aligned exactly 1 time 4498109 (22.57%) aligned >1 times 98.31% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4748840 / 19591262 = 0.2424 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:29:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:29:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:29:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:29:53: 1000000 INFO @ Sun, 21 Jun 2020 21:29:59: 2000000 INFO @ Sun, 21 Jun 2020 21:30:04: 3000000 INFO @ Sun, 21 Jun 2020 21:30:09: 4000000 INFO @ Sun, 21 Jun 2020 21:30:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:30:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:30:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:30:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:30:20: 6000000 INFO @ Sun, 21 Jun 2020 21:30:25: 1000000 INFO @ Sun, 21 Jun 2020 21:30:26: 7000000 INFO @ Sun, 21 Jun 2020 21:30:31: 2000000 INFO @ Sun, 21 Jun 2020 21:30:32: 8000000 INFO @ Sun, 21 Jun 2020 21:30:38: 3000000 INFO @ Sun, 21 Jun 2020 21:30:39: 9000000 INFO @ Sun, 21 Jun 2020 21:30:45: 10000000 INFO @ Sun, 21 Jun 2020 21:30:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:30:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:30:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:30:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:30:51: 11000000 INFO @ Sun, 21 Jun 2020 21:30:52: 5000000 INFO @ Sun, 21 Jun 2020 21:30:54: 1000000 INFO @ Sun, 21 Jun 2020 21:30:57: 12000000 INFO @ Sun, 21 Jun 2020 21:30:59: 6000000 INFO @ Sun, 21 Jun 2020 21:31:00: 2000000 INFO @ Sun, 21 Jun 2020 21:31:04: 13000000 INFO @ Sun, 21 Jun 2020 21:31:05: 7000000 INFO @ Sun, 21 Jun 2020 21:31:07: 3000000 INFO @ Sun, 21 Jun 2020 21:31:10: 14000000 INFO @ Sun, 21 Jun 2020 21:31:12: 8000000 INFO @ Sun, 21 Jun 2020 21:31:13: 4000000 INFO @ Sun, 21 Jun 2020 21:31:16: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:31:16: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:31:16: #1 total tags in treatment: 14842422 INFO @ Sun, 21 Jun 2020 21:31:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:31:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:31:16: #1 tags after filtering in treatment: 14842420 INFO @ Sun, 21 Jun 2020 21:31:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:31:16: #1 finished! INFO @ Sun, 21 Jun 2020 21:31:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:31:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:31:17: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:31:17: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:31:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:31:19: 9000000 INFO @ Sun, 21 Jun 2020 21:31:19: 5000000 INFO @ Sun, 21 Jun 2020 21:31:26: 6000000 INFO @ Sun, 21 Jun 2020 21:31:26: 10000000 INFO @ Sun, 21 Jun 2020 21:31:32: 7000000 INFO @ Sun, 21 Jun 2020 21:31:33: 11000000 INFO @ Sun, 21 Jun 2020 21:31:38: 8000000 INFO @ Sun, 21 Jun 2020 21:31:40: 12000000 INFO @ Sun, 21 Jun 2020 21:31:44: 9000000 INFO @ Sun, 21 Jun 2020 21:31:47: 13000000 INFO @ Sun, 21 Jun 2020 21:31:51: 10000000 INFO @ Sun, 21 Jun 2020 21:31:54: 14000000 INFO @ Sun, 21 Jun 2020 21:31:57: 11000000 INFO @ Sun, 21 Jun 2020 21:32:01: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:32:01: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:32:01: #1 total tags in treatment: 14842422 INFO @ Sun, 21 Jun 2020 21:32:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:32:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:32:01: #1 tags after filtering in treatment: 14842420 INFO @ Sun, 21 Jun 2020 21:32:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:32:01: #1 finished! INFO @ Sun, 21 Jun 2020 21:32:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:32:01: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:32:02: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:32:02: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:32:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:32:03: 12000000 INFO @ Sun, 21 Jun 2020 21:32:09: 13000000 INFO @ Sun, 21 Jun 2020 21:32:14: 14000000 INFO @ Sun, 21 Jun 2020 21:32:19: #1 tag size is determined as 44 bps INFO @ Sun, 21 Jun 2020 21:32:19: #1 tag size = 44 INFO @ Sun, 21 Jun 2020 21:32:19: #1 total tags in treatment: 14842422 INFO @ Sun, 21 Jun 2020 21:32:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:32:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:32:20: #1 tags after filtering in treatment: 14842420 INFO @ Sun, 21 Jun 2020 21:32:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:32:20: #1 finished! INFO @ Sun, 21 Jun 2020 21:32:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:32:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:32:21: #2 number of paired peaks: 81 WARNING @ Sun, 21 Jun 2020 21:32:21: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:32:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495776/SRX495776.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。