Job ID = 6458218 SRX = SRX495324 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:24:04 prefetch.2.10.7: 1) Downloading 'SRR1198829'... 2020-06-21T12:24:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:31:35 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:31:35 prefetch.2.10.7: 1) 'SRR1198829' was downloaded successfully Read 36935373 spots for SRR1198829/SRR1198829.sra Written 36935373 spots for SRR1198829/SRR1198829.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:55 36935373 reads; of these: 36935373 (100.00%) were unpaired; of these: 3747216 (10.15%) aligned 0 times 23869104 (64.62%) aligned exactly 1 time 9319053 (25.23%) aligned >1 times 89.85% overall alignment rate Time searching: 00:10:55 Overall time: 00:10:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9811182 / 33188157 = 0.2956 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:51:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:51:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:51:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:51:37: 1000000 INFO @ Sun, 21 Jun 2020 21:51:42: 2000000 INFO @ Sun, 21 Jun 2020 21:51:48: 3000000 INFO @ Sun, 21 Jun 2020 21:51:53: 4000000 INFO @ Sun, 21 Jun 2020 21:51:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:05: 6000000 INFO @ Sun, 21 Jun 2020 21:52:07: 1000000 INFO @ Sun, 21 Jun 2020 21:52:11: 7000000 INFO @ Sun, 21 Jun 2020 21:52:13: 2000000 INFO @ Sun, 21 Jun 2020 21:52:17: 8000000 INFO @ Sun, 21 Jun 2020 21:52:18: 3000000 INFO @ Sun, 21 Jun 2020 21:52:22: 9000000 INFO @ Sun, 21 Jun 2020 21:52:24: 4000000 INFO @ Sun, 21 Jun 2020 21:52:28: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:30: 5000000 INFO @ Sun, 21 Jun 2020 21:52:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:34: 11000000 INFO @ Sun, 21 Jun 2020 21:52:35: 6000000 INFO @ Sun, 21 Jun 2020 21:52:37: 1000000 INFO @ Sun, 21 Jun 2020 21:52:39: 12000000 INFO @ Sun, 21 Jun 2020 21:52:41: 7000000 INFO @ Sun, 21 Jun 2020 21:52:43: 2000000 INFO @ Sun, 21 Jun 2020 21:52:45: 13000000 INFO @ Sun, 21 Jun 2020 21:52:47: 8000000 INFO @ Sun, 21 Jun 2020 21:52:49: 3000000 INFO @ Sun, 21 Jun 2020 21:52:51: 14000000 INFO @ Sun, 21 Jun 2020 21:52:53: 9000000 INFO @ Sun, 21 Jun 2020 21:52:55: 4000000 INFO @ Sun, 21 Jun 2020 21:52:57: 15000000 INFO @ Sun, 21 Jun 2020 21:52:59: 10000000 INFO @ Sun, 21 Jun 2020 21:53:01: 5000000 INFO @ Sun, 21 Jun 2020 21:53:02: 16000000 INFO @ Sun, 21 Jun 2020 21:53:05: 11000000 INFO @ Sun, 21 Jun 2020 21:53:07: 6000000 INFO @ Sun, 21 Jun 2020 21:53:08: 17000000 INFO @ Sun, 21 Jun 2020 21:53:10: 12000000 INFO @ Sun, 21 Jun 2020 21:53:13: 7000000 INFO @ Sun, 21 Jun 2020 21:53:14: 18000000 INFO @ Sun, 21 Jun 2020 21:53:16: 13000000 INFO @ Sun, 21 Jun 2020 21:53:18: 8000000 INFO @ Sun, 21 Jun 2020 21:53:20: 19000000 INFO @ Sun, 21 Jun 2020 21:53:22: 14000000 INFO @ Sun, 21 Jun 2020 21:53:24: 9000000 INFO @ Sun, 21 Jun 2020 21:53:26: 20000000 INFO @ Sun, 21 Jun 2020 21:53:28: 15000000 INFO @ Sun, 21 Jun 2020 21:53:30: 10000000 INFO @ Sun, 21 Jun 2020 21:53:32: 21000000 INFO @ Sun, 21 Jun 2020 21:53:33: 16000000 INFO @ Sun, 21 Jun 2020 21:53:36: 11000000 INFO @ Sun, 21 Jun 2020 21:53:38: 22000000 INFO @ Sun, 21 Jun 2020 21:53:39: 17000000 INFO @ Sun, 21 Jun 2020 21:53:42: 12000000 INFO @ Sun, 21 Jun 2020 21:53:44: 23000000 INFO @ Sun, 21 Jun 2020 21:53:45: 18000000 INFO @ Sun, 21 Jun 2020 21:53:46: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:53:46: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:53:46: #1 total tags in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:53:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:53:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:53:47: #1 tags after filtering in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:53:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:53:47: #1 finished! INFO @ Sun, 21 Jun 2020 21:53:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:53:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:53:47: 13000000 INFO @ Sun, 21 Jun 2020 21:53:48: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:53:48: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:53:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:53:52: 19000000 INFO @ Sun, 21 Jun 2020 21:53:53: 14000000 INFO @ Sun, 21 Jun 2020 21:53:57: 20000000 INFO @ Sun, 21 Jun 2020 21:53:59: 15000000 INFO @ Sun, 21 Jun 2020 21:54:03: 21000000 INFO @ Sun, 21 Jun 2020 21:54:04: 16000000 INFO @ Sun, 21 Jun 2020 21:54:09: 22000000 INFO @ Sun, 21 Jun 2020 21:54:10: 17000000 INFO @ Sun, 21 Jun 2020 21:54:15: 23000000 INFO @ Sun, 21 Jun 2020 21:54:16: 18000000 INFO @ Sun, 21 Jun 2020 21:54:18: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:54:18: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:54:18: #1 total tags in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:54:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:54:18: #1 tags after filtering in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:54:18: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:54:18: #1 finished! INFO @ Sun, 21 Jun 2020 21:54:18: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:54:18: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:54:20: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:54:20: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:54:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:54:22: 19000000 INFO @ Sun, 21 Jun 2020 21:54:28: 20000000 INFO @ Sun, 21 Jun 2020 21:54:34: 21000000 INFO @ Sun, 21 Jun 2020 21:54:39: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:54:45: 23000000 INFO @ Sun, 21 Jun 2020 21:54:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:54:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:54:48: #1 total tags in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:54:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:54:48: #1 tags after filtering in treatment: 23376975 INFO @ Sun, 21 Jun 2020 21:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:54:48: #1 finished! INFO @ Sun, 21 Jun 2020 21:54:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:54:50: #2 number of paired peaks: 76 WARNING @ Sun, 21 Jun 2020 21:54:50: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:54:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495324/SRX495324.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。