Job ID = 6458216 SRX = SRX495322 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:14:34 prefetch.2.10.7: 1) Downloading 'SRR1198827'... 2020-06-21T12:14:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:18:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:18:19 prefetch.2.10.7: 1) 'SRR1198827' was downloaded successfully Read 32482612 spots for SRR1198827/SRR1198827.sra Written 32482612 spots for SRR1198827/SRR1198827.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:48 32482612 reads; of these: 32482612 (100.00%) were unpaired; of these: 1982636 (6.10%) aligned 0 times 22017503 (67.78%) aligned exactly 1 time 8482473 (26.11%) aligned >1 times 93.90% overall alignment rate Time searching: 00:08:48 Overall time: 00:08:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9842896 / 30499976 = 0.3227 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:15: 1000000 INFO @ Sun, 21 Jun 2020 21:35:21: 2000000 INFO @ Sun, 21 Jun 2020 21:35:26: 3000000 INFO @ Sun, 21 Jun 2020 21:35:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:35:38: 5000000 INFO @ Sun, 21 Jun 2020 21:35:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:35:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:35:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:35:44: 6000000 INFO @ Sun, 21 Jun 2020 21:35:45: 1000000 INFO @ Sun, 21 Jun 2020 21:35:51: 7000000 INFO @ Sun, 21 Jun 2020 21:35:52: 2000000 INFO @ Sun, 21 Jun 2020 21:35:57: 8000000 INFO @ Sun, 21 Jun 2020 21:35:58: 3000000 INFO @ Sun, 21 Jun 2020 21:36:03: 9000000 INFO @ Sun, 21 Jun 2020 21:36:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:10: 10000000 INFO @ Sun, 21 Jun 2020 21:36:11: 5000000 INFO @ Sun, 21 Jun 2020 21:36:16: 1000000 INFO @ Sun, 21 Jun 2020 21:36:16: 11000000 INFO @ Sun, 21 Jun 2020 21:36:17: 6000000 INFO @ Sun, 21 Jun 2020 21:36:22: 2000000 INFO @ Sun, 21 Jun 2020 21:36:23: 12000000 INFO @ Sun, 21 Jun 2020 21:36:24: 7000000 INFO @ Sun, 21 Jun 2020 21:36:29: 3000000 INFO @ Sun, 21 Jun 2020 21:36:29: 13000000 INFO @ Sun, 21 Jun 2020 21:36:30: 8000000 INFO @ Sun, 21 Jun 2020 21:36:35: 4000000 INFO @ Sun, 21 Jun 2020 21:36:36: 14000000 INFO @ Sun, 21 Jun 2020 21:36:37: 9000000 INFO @ Sun, 21 Jun 2020 21:36:42: 5000000 INFO @ Sun, 21 Jun 2020 21:36:42: 15000000 INFO @ Sun, 21 Jun 2020 21:36:43: 10000000 INFO @ Sun, 21 Jun 2020 21:36:48: 6000000 INFO @ Sun, 21 Jun 2020 21:36:49: 16000000 INFO @ Sun, 21 Jun 2020 21:36:50: 11000000 INFO @ Sun, 21 Jun 2020 21:36:55: 7000000 INFO @ Sun, 21 Jun 2020 21:36:56: 17000000 INFO @ Sun, 21 Jun 2020 21:36:56: 12000000 INFO @ Sun, 21 Jun 2020 21:37:01: 8000000 INFO @ Sun, 21 Jun 2020 21:37:02: 18000000 INFO @ Sun, 21 Jun 2020 21:37:03: 13000000 INFO @ Sun, 21 Jun 2020 21:37:08: 9000000 INFO @ Sun, 21 Jun 2020 21:37:08: 19000000 INFO @ Sun, 21 Jun 2020 21:37:09: 14000000 INFO @ Sun, 21 Jun 2020 21:37:14: 10000000 INFO @ Sun, 21 Jun 2020 21:37:15: 20000000 INFO @ Sun, 21 Jun 2020 21:37:16: 15000000 INFO @ Sun, 21 Jun 2020 21:37:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:37:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:37:20: #1 total tags in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:37:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:20: #1 tags after filtering in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:37:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:20: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:21: 11000000 INFO @ Sun, 21 Jun 2020 21:37:21: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:37:21: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:22: 16000000 INFO @ Sun, 21 Jun 2020 21:37:27: 12000000 INFO @ Sun, 21 Jun 2020 21:37:29: 17000000 INFO @ Sun, 21 Jun 2020 21:37:34: 13000000 INFO @ Sun, 21 Jun 2020 21:37:35: 18000000 INFO @ Sun, 21 Jun 2020 21:37:40: 14000000 INFO @ Sun, 21 Jun 2020 21:37:42: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:37:47: 15000000 INFO @ Sun, 21 Jun 2020 21:37:48: 20000000 INFO @ Sun, 21 Jun 2020 21:37:53: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:37:53: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:37:53: #1 total tags in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:37:53: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:37:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:37:53: 16000000 INFO @ Sun, 21 Jun 2020 21:37:53: #1 tags after filtering in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:37:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:37:53: #1 finished! INFO @ Sun, 21 Jun 2020 21:37:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:37:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:37:55: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:37:55: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:37:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:37:59: 17000000 INFO @ Sun, 21 Jun 2020 21:38:05: 18000000 INFO @ Sun, 21 Jun 2020 21:38:11: 19000000 INFO @ Sun, 21 Jun 2020 21:38:16: 20000000 INFO @ Sun, 21 Jun 2020 21:38:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:38:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:38:20: #1 total tags in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:38:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:38:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:38:21: #1 tags after filtering in treatment: 20657080 INFO @ Sun, 21 Jun 2020 21:38:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:38:21: #1 finished! INFO @ Sun, 21 Jun 2020 21:38:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:38:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:38:22: #2 number of paired peaks: 96 WARNING @ Sun, 21 Jun 2020 21:38:22: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:38:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495322/SRX495322.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。