Job ID = 6458209 SRX = SRX495316 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:20:04 prefetch.2.10.7: 1) Downloading 'SRR1198821'... 2020-06-21T12:20:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:24:12 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:24:12 prefetch.2.10.7: 1) 'SRR1198821' was downloaded successfully Read 55261147 spots for SRR1198821/SRR1198821.sra Written 55261147 spots for SRR1198821/SRR1198821.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:55 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1196343 (2.16%) aligned 0 times 45510293 (82.35%) aligned exactly 1 time 8554511 (15.48%) aligned >1 times 97.84% overall alignment rate Time searching: 00:12:55 Overall time: 00:12:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17944452 / 54064804 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:50:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:50:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:50:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:50:23: 1000000 INFO @ Sun, 21 Jun 2020 21:50:28: 2000000 INFO @ Sun, 21 Jun 2020 21:50:33: 3000000 INFO @ Sun, 21 Jun 2020 21:50:38: 4000000 INFO @ Sun, 21 Jun 2020 21:50:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:50:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:50:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:50:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:50:48: 6000000 INFO @ Sun, 21 Jun 2020 21:50:53: 1000000 INFO @ Sun, 21 Jun 2020 21:50:54: 7000000 INFO @ Sun, 21 Jun 2020 21:50:59: 2000000 INFO @ Sun, 21 Jun 2020 21:50:59: 8000000 INFO @ Sun, 21 Jun 2020 21:51:04: 3000000 INFO @ Sun, 21 Jun 2020 21:51:04: 9000000 INFO @ Sun, 21 Jun 2020 21:51:09: 4000000 INFO @ Sun, 21 Jun 2020 21:51:09: 10000000 INFO @ Sun, 21 Jun 2020 21:51:14: 5000000 INFO @ Sun, 21 Jun 2020 21:51:14: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:51:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:51:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:51:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:51:19: 6000000 INFO @ Sun, 21 Jun 2020 21:51:20: 12000000 INFO @ Sun, 21 Jun 2020 21:51:23: 1000000 INFO @ Sun, 21 Jun 2020 21:51:25: 7000000 INFO @ Sun, 21 Jun 2020 21:51:25: 13000000 INFO @ Sun, 21 Jun 2020 21:51:29: 2000000 INFO @ Sun, 21 Jun 2020 21:51:31: 8000000 INFO @ Sun, 21 Jun 2020 21:51:31: 14000000 INFO @ Sun, 21 Jun 2020 21:51:34: 3000000 INFO @ Sun, 21 Jun 2020 21:51:36: 9000000 INFO @ Sun, 21 Jun 2020 21:51:36: 15000000 INFO @ Sun, 21 Jun 2020 21:51:40: 4000000 INFO @ Sun, 21 Jun 2020 21:51:41: 16000000 INFO @ Sun, 21 Jun 2020 21:51:41: 10000000 INFO @ Sun, 21 Jun 2020 21:51:45: 5000000 INFO @ Sun, 21 Jun 2020 21:51:47: 17000000 INFO @ Sun, 21 Jun 2020 21:51:47: 11000000 INFO @ Sun, 21 Jun 2020 21:51:50: 6000000 INFO @ Sun, 21 Jun 2020 21:51:52: 18000000 INFO @ Sun, 21 Jun 2020 21:51:52: 12000000 INFO @ Sun, 21 Jun 2020 21:51:56: 7000000 INFO @ Sun, 21 Jun 2020 21:51:58: 19000000 INFO @ Sun, 21 Jun 2020 21:51:58: 13000000 INFO @ Sun, 21 Jun 2020 21:52:02: 8000000 INFO @ Sun, 21 Jun 2020 21:52:03: 20000000 INFO @ Sun, 21 Jun 2020 21:52:04: 14000000 INFO @ Sun, 21 Jun 2020 21:52:07: 9000000 INFO @ Sun, 21 Jun 2020 21:52:09: 21000000 INFO @ Sun, 21 Jun 2020 21:52:09: 15000000 INFO @ Sun, 21 Jun 2020 21:52:13: 10000000 INFO @ Sun, 21 Jun 2020 21:52:14: 22000000 INFO @ Sun, 21 Jun 2020 21:52:15: 16000000 INFO @ Sun, 21 Jun 2020 21:52:18: 11000000 INFO @ Sun, 21 Jun 2020 21:52:20: 23000000 INFO @ Sun, 21 Jun 2020 21:52:20: 17000000 INFO @ Sun, 21 Jun 2020 21:52:24: 12000000 INFO @ Sun, 21 Jun 2020 21:52:25: 24000000 INFO @ Sun, 21 Jun 2020 21:52:26: 18000000 INFO @ Sun, 21 Jun 2020 21:52:29: 13000000 INFO @ Sun, 21 Jun 2020 21:52:31: 25000000 INFO @ Sun, 21 Jun 2020 21:52:31: 19000000 INFO @ Sun, 21 Jun 2020 21:52:35: 14000000 INFO @ Sun, 21 Jun 2020 21:52:36: 26000000 INFO @ Sun, 21 Jun 2020 21:52:37: 20000000 INFO @ Sun, 21 Jun 2020 21:52:40: 15000000 INFO @ Sun, 21 Jun 2020 21:52:42: 27000000 INFO @ Sun, 21 Jun 2020 21:52:42: 21000000 INFO @ Sun, 21 Jun 2020 21:52:45: 16000000 INFO @ Sun, 21 Jun 2020 21:52:47: 28000000 INFO @ Sun, 21 Jun 2020 21:52:47: 22000000 INFO @ Sun, 21 Jun 2020 21:52:51: 17000000 INFO @ Sun, 21 Jun 2020 21:52:53: 29000000 INFO @ Sun, 21 Jun 2020 21:52:53: 23000000 INFO @ Sun, 21 Jun 2020 21:52:56: 18000000 INFO @ Sun, 21 Jun 2020 21:52:58: 24000000 INFO @ Sun, 21 Jun 2020 21:52:58: 30000000 INFO @ Sun, 21 Jun 2020 21:53:01: 19000000 INFO @ Sun, 21 Jun 2020 21:53:04: 25000000 INFO @ Sun, 21 Jun 2020 21:53:04: 31000000 INFO @ Sun, 21 Jun 2020 21:53:07: 20000000 INFO @ Sun, 21 Jun 2020 21:53:09: 26000000 INFO @ Sun, 21 Jun 2020 21:53:10: 32000000 INFO @ Sun, 21 Jun 2020 21:53:12: 21000000 INFO @ Sun, 21 Jun 2020 21:53:15: 27000000 INFO @ Sun, 21 Jun 2020 21:53:15: 33000000 INFO @ Sun, 21 Jun 2020 21:53:17: 22000000 INFO @ Sun, 21 Jun 2020 21:53:20: 28000000 INFO @ Sun, 21 Jun 2020 21:53:21: 34000000 INFO @ Sun, 21 Jun 2020 21:53:23: 23000000 INFO @ Sun, 21 Jun 2020 21:53:25: 29000000 INFO @ Sun, 21 Jun 2020 21:53:26: 35000000 INFO @ Sun, 21 Jun 2020 21:53:28: 24000000 INFO @ Sun, 21 Jun 2020 21:53:32: 30000000 INFO @ Sun, 21 Jun 2020 21:53:32: 36000000 INFO @ Sun, 21 Jun 2020 21:53:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:53:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:53:33: #1 total tags in treatment: 36120352 INFO @ Sun, 21 Jun 2020 21:53:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:53:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:53:34: #1 tags after filtering in treatment: 36120351 INFO @ Sun, 21 Jun 2020 21:53:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:53:34: #1 finished! INFO @ Sun, 21 Jun 2020 21:53:34: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:53:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:53:34: 25000000 INFO @ Sun, 21 Jun 2020 21:53:36: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:53:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:53:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:53:37: 31000000 INFO @ Sun, 21 Jun 2020 21:53:39: 26000000 INFO @ Sun, 21 Jun 2020 21:53:42: 32000000 INFO @ Sun, 21 Jun 2020 21:53:44: 27000000 INFO @ Sun, 21 Jun 2020 21:53:47: 33000000 INFO @ Sun, 21 Jun 2020 21:53:49: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:53:53: 34000000 INFO @ Sun, 21 Jun 2020 21:53:54: 29000000 INFO @ Sun, 21 Jun 2020 21:53:58: 35000000 INFO @ Sun, 21 Jun 2020 21:54:00: 30000000 INFO @ Sun, 21 Jun 2020 21:54:03: 36000000 INFO @ Sun, 21 Jun 2020 21:54:04: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:54:04: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:54:04: #1 total tags in treatment: 36120352 INFO @ Sun, 21 Jun 2020 21:54:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:54:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:54:05: #1 tags after filtering in treatment: 36120351 INFO @ Sun, 21 Jun 2020 21:54:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:54:05: #1 finished! INFO @ Sun, 21 Jun 2020 21:54:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:54:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:54:05: 31000000 INFO @ Sun, 21 Jun 2020 21:54:07: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:54:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:54:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:54:10: 32000000 INFO @ Sun, 21 Jun 2020 21:54:15: 33000000 INFO @ Sun, 21 Jun 2020 21:54:20: 34000000 INFO @ Sun, 21 Jun 2020 21:54:25: 35000000 INFO @ Sun, 21 Jun 2020 21:54:30: 36000000 INFO @ Sun, 21 Jun 2020 21:54:31: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:54:31: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:54:31: #1 total tags in treatment: 36120352 INFO @ Sun, 21 Jun 2020 21:54:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:54:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:54:32: #1 tags after filtering in treatment: 36120351 INFO @ Sun, 21 Jun 2020 21:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:54:32: #1 finished! INFO @ Sun, 21 Jun 2020 21:54:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:54:34: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:54:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:54:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495316/SRX495316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。