Job ID = 6458206 SRX = SRX495315 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:17:49 prefetch.2.10.7: 1) Downloading 'SRR1198820'... 2020-06-21T12:17:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:23:07 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:23:07 prefetch.2.10.7: 1) 'SRR1198820' was downloaded successfully Read 50358398 spots for SRR1198820/SRR1198820.sra Written 50358398 spots for SRR1198820/SRR1198820.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:38 50358398 reads; of these: 50358398 (100.00%) were unpaired; of these: 1099663 (2.18%) aligned 0 times 41930346 (83.26%) aligned exactly 1 time 7328389 (14.55%) aligned >1 times 97.82% overall alignment rate Time searching: 00:11:38 Overall time: 00:11:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 15706684 / 49258735 = 0.3189 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:16: 1000000 INFO @ Sun, 21 Jun 2020 21:47:21: 2000000 INFO @ Sun, 21 Jun 2020 21:47:26: 3000000 INFO @ Sun, 21 Jun 2020 21:47:31: 4000000 INFO @ Sun, 21 Jun 2020 21:47:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:40: 6000000 INFO @ Sun, 21 Jun 2020 21:47:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:46: 7000000 INFO @ Sun, 21 Jun 2020 21:47:46: 1000000 INFO @ Sun, 21 Jun 2020 21:47:51: 8000000 INFO @ Sun, 21 Jun 2020 21:47:51: 2000000 INFO @ Sun, 21 Jun 2020 21:47:56: 9000000 INFO @ Sun, 21 Jun 2020 21:47:57: 3000000 INFO @ Sun, 21 Jun 2020 21:48:01: 10000000 INFO @ Sun, 21 Jun 2020 21:48:02: 4000000 INFO @ Sun, 21 Jun 2020 21:48:06: 11000000 INFO @ Sun, 21 Jun 2020 21:48:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:48:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:48:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:48:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:48:11: 12000000 INFO @ Sun, 21 Jun 2020 21:48:12: 6000000 INFO @ Sun, 21 Jun 2020 21:48:16: 1000000 INFO @ Sun, 21 Jun 2020 21:48:17: 13000000 INFO @ Sun, 21 Jun 2020 21:48:18: 7000000 INFO @ Sun, 21 Jun 2020 21:48:21: 2000000 INFO @ Sun, 21 Jun 2020 21:48:22: 14000000 INFO @ Sun, 21 Jun 2020 21:48:23: 8000000 INFO @ Sun, 21 Jun 2020 21:48:27: 3000000 INFO @ Sun, 21 Jun 2020 21:48:27: 15000000 INFO @ Sun, 21 Jun 2020 21:48:28: 9000000 INFO @ Sun, 21 Jun 2020 21:48:32: 4000000 INFO @ Sun, 21 Jun 2020 21:48:32: 16000000 INFO @ Sun, 21 Jun 2020 21:48:34: 10000000 INFO @ Sun, 21 Jun 2020 21:48:37: 5000000 INFO @ Sun, 21 Jun 2020 21:48:38: 17000000 INFO @ Sun, 21 Jun 2020 21:48:39: 11000000 INFO @ Sun, 21 Jun 2020 21:48:43: 6000000 INFO @ Sun, 21 Jun 2020 21:48:43: 18000000 INFO @ Sun, 21 Jun 2020 21:48:44: 12000000 INFO @ Sun, 21 Jun 2020 21:48:48: 7000000 INFO @ Sun, 21 Jun 2020 21:48:48: 19000000 INFO @ Sun, 21 Jun 2020 21:48:49: 13000000 INFO @ Sun, 21 Jun 2020 21:48:53: 8000000 INFO @ Sun, 21 Jun 2020 21:48:53: 20000000 INFO @ Sun, 21 Jun 2020 21:48:55: 14000000 INFO @ Sun, 21 Jun 2020 21:48:58: 9000000 INFO @ Sun, 21 Jun 2020 21:48:59: 21000000 INFO @ Sun, 21 Jun 2020 21:49:00: 15000000 INFO @ Sun, 21 Jun 2020 21:49:04: 10000000 INFO @ Sun, 21 Jun 2020 21:49:04: 22000000 INFO @ Sun, 21 Jun 2020 21:49:05: 16000000 INFO @ Sun, 21 Jun 2020 21:49:09: 11000000 INFO @ Sun, 21 Jun 2020 21:49:10: 23000000 INFO @ Sun, 21 Jun 2020 21:49:10: 17000000 INFO @ Sun, 21 Jun 2020 21:49:14: 12000000 INFO @ Sun, 21 Jun 2020 21:49:15: 24000000 INFO @ Sun, 21 Jun 2020 21:49:16: 18000000 INFO @ Sun, 21 Jun 2020 21:49:20: 13000000 INFO @ Sun, 21 Jun 2020 21:49:20: 25000000 INFO @ Sun, 21 Jun 2020 21:49:21: 19000000 INFO @ Sun, 21 Jun 2020 21:49:25: 14000000 INFO @ Sun, 21 Jun 2020 21:49:25: 26000000 INFO @ Sun, 21 Jun 2020 21:49:26: 20000000 INFO @ Sun, 21 Jun 2020 21:49:30: 15000000 INFO @ Sun, 21 Jun 2020 21:49:31: 27000000 INFO @ Sun, 21 Jun 2020 21:49:32: 21000000 INFO @ Sun, 21 Jun 2020 21:49:35: 16000000 INFO @ Sun, 21 Jun 2020 21:49:36: 28000000 INFO @ Sun, 21 Jun 2020 21:49:37: 22000000 INFO @ Sun, 21 Jun 2020 21:49:41: 17000000 INFO @ Sun, 21 Jun 2020 21:49:42: 29000000 INFO @ Sun, 21 Jun 2020 21:49:42: 23000000 INFO @ Sun, 21 Jun 2020 21:49:46: 18000000 INFO @ Sun, 21 Jun 2020 21:49:47: 30000000 INFO @ Sun, 21 Jun 2020 21:49:48: 24000000 INFO @ Sun, 21 Jun 2020 21:49:51: 19000000 INFO @ Sun, 21 Jun 2020 21:49:52: 31000000 INFO @ Sun, 21 Jun 2020 21:49:53: 25000000 INFO @ Sun, 21 Jun 2020 21:49:56: 20000000 INFO @ Sun, 21 Jun 2020 21:49:58: 32000000 INFO @ Sun, 21 Jun 2020 21:49:58: 26000000 INFO @ Sun, 21 Jun 2020 21:50:02: 21000000 INFO @ Sun, 21 Jun 2020 21:50:03: 33000000 INFO @ Sun, 21 Jun 2020 21:50:03: 27000000 INFO @ Sun, 21 Jun 2020 21:50:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:50:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:50:07: #1 total tags in treatment: 33552051 INFO @ Sun, 21 Jun 2020 21:50:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:07: 22000000 INFO @ Sun, 21 Jun 2020 21:50:07: #1 tags after filtering in treatment: 33552048 INFO @ Sun, 21 Jun 2020 21:50:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:07: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:09: 28000000 INFO @ Sun, 21 Jun 2020 21:50:10: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:50:10: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:50:12: 23000000 INFO @ Sun, 21 Jun 2020 21:50:14: 29000000 INFO @ Sun, 21 Jun 2020 21:50:18: 24000000 INFO @ Sun, 21 Jun 2020 21:50:19: 30000000 INFO @ Sun, 21 Jun 2020 21:50:23: 25000000 INFO @ Sun, 21 Jun 2020 21:50:25: 31000000 INFO @ Sun, 21 Jun 2020 21:50:28: 26000000 INFO @ Sun, 21 Jun 2020 21:50:30: 32000000 INFO @ Sun, 21 Jun 2020 21:50:33: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:50:36: 33000000 INFO @ Sun, 21 Jun 2020 21:50:39: 28000000 INFO @ Sun, 21 Jun 2020 21:50:39: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:50:39: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:50:39: #1 total tags in treatment: 33552051 INFO @ Sun, 21 Jun 2020 21:50:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:40: #1 tags after filtering in treatment: 33552048 INFO @ Sun, 21 Jun 2020 21:50:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:40: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:42: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:50:42: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:50:44: 29000000 INFO @ Sun, 21 Jun 2020 21:50:49: 30000000 INFO @ Sun, 21 Jun 2020 21:50:54: 31000000 INFO @ Sun, 21 Jun 2020 21:50:59: 32000000 INFO @ Sun, 21 Jun 2020 21:51:04: 33000000 INFO @ Sun, 21 Jun 2020 21:51:08: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:51:08: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:51:08: #1 total tags in treatment: 33552051 INFO @ Sun, 21 Jun 2020 21:51:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:51:08: #1 tags after filtering in treatment: 33552048 INFO @ Sun, 21 Jun 2020 21:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:51:08: #1 finished! INFO @ Sun, 21 Jun 2020 21:51:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:51:10: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:51:10: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:51:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495315/SRX495315.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。