Job ID = 6458205 SRX = SRX495314 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:19:04 prefetch.2.10.7: 1) Downloading 'SRR1198819'... 2020-06-21T12:19:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:27:02 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:27:02 prefetch.2.10.7: 1) 'SRR1198819' was downloaded successfully Read 55261147 spots for SRR1198819/SRR1198819.sra Written 55261147 spots for SRR1198819/SRR1198819.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:55 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1196325 (2.16%) aligned 0 times 45510169 (82.35%) aligned exactly 1 time 8554653 (15.48%) aligned >1 times 97.84% overall alignment rate Time searching: 00:13:55 Overall time: 00:13:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17943874 / 54064822 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:55:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:55:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:55:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:55:56: 1000000 INFO @ Sun, 21 Jun 2020 21:56:01: 2000000 INFO @ Sun, 21 Jun 2020 21:56:06: 3000000 INFO @ Sun, 21 Jun 2020 21:56:12: 4000000 INFO @ Sun, 21 Jun 2020 21:56:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:56:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:56:20: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:56:20: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:56:22: 6000000 INFO @ Sun, 21 Jun 2020 21:56:27: 1000000 INFO @ Sun, 21 Jun 2020 21:56:28: 7000000 INFO @ Sun, 21 Jun 2020 21:56:33: 2000000 INFO @ Sun, 21 Jun 2020 21:56:34: 8000000 INFO @ Sun, 21 Jun 2020 21:56:40: 9000000 INFO @ Sun, 21 Jun 2020 21:56:40: 3000000 INFO @ Sun, 21 Jun 2020 21:56:46: 10000000 INFO @ Sun, 21 Jun 2020 21:56:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:56:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:56:50: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:56:50: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:56:51: 11000000 INFO @ Sun, 21 Jun 2020 21:56:53: 5000000 INFO @ Sun, 21 Jun 2020 21:56:57: 1000000 INFO @ Sun, 21 Jun 2020 21:56:57: 12000000 INFO @ Sun, 21 Jun 2020 21:57:00: 6000000 INFO @ Sun, 21 Jun 2020 21:57:03: 13000000 INFO @ Sun, 21 Jun 2020 21:57:04: 2000000 INFO @ Sun, 21 Jun 2020 21:57:06: 7000000 INFO @ Sun, 21 Jun 2020 21:57:09: 14000000 INFO @ Sun, 21 Jun 2020 21:57:10: 3000000 INFO @ Sun, 21 Jun 2020 21:57:13: 8000000 INFO @ Sun, 21 Jun 2020 21:57:15: 15000000 INFO @ Sun, 21 Jun 2020 21:57:17: 4000000 INFO @ Sun, 21 Jun 2020 21:57:19: 9000000 INFO @ Sun, 21 Jun 2020 21:57:21: 16000000 INFO @ Sun, 21 Jun 2020 21:57:23: 5000000 INFO @ Sun, 21 Jun 2020 21:57:25: 10000000 INFO @ Sun, 21 Jun 2020 21:57:26: 17000000 INFO @ Sun, 21 Jun 2020 21:57:30: 6000000 INFO @ Sun, 21 Jun 2020 21:57:32: 11000000 INFO @ Sun, 21 Jun 2020 21:57:32: 18000000 INFO @ Sun, 21 Jun 2020 21:57:36: 7000000 INFO @ Sun, 21 Jun 2020 21:57:38: 19000000 INFO @ Sun, 21 Jun 2020 21:57:38: 12000000 INFO @ Sun, 21 Jun 2020 21:57:42: 8000000 INFO @ Sun, 21 Jun 2020 21:57:44: 20000000 INFO @ Sun, 21 Jun 2020 21:57:45: 13000000 INFO @ Sun, 21 Jun 2020 21:57:49: 9000000 INFO @ Sun, 21 Jun 2020 21:57:50: 21000000 INFO @ Sun, 21 Jun 2020 21:57:51: 14000000 INFO @ Sun, 21 Jun 2020 21:57:55: 10000000 INFO @ Sun, 21 Jun 2020 21:57:56: 22000000 INFO @ Sun, 21 Jun 2020 21:57:57: 15000000 INFO @ Sun, 21 Jun 2020 21:58:01: 11000000 INFO @ Sun, 21 Jun 2020 21:58:02: 23000000 INFO @ Sun, 21 Jun 2020 21:58:04: 16000000 INFO @ Sun, 21 Jun 2020 21:58:07: 24000000 INFO @ Sun, 21 Jun 2020 21:58:07: 12000000 INFO @ Sun, 21 Jun 2020 21:58:10: 17000000 INFO @ Sun, 21 Jun 2020 21:58:13: 25000000 INFO @ Sun, 21 Jun 2020 21:58:14: 13000000 INFO @ Sun, 21 Jun 2020 21:58:16: 18000000 INFO @ Sun, 21 Jun 2020 21:58:19: 26000000 INFO @ Sun, 21 Jun 2020 21:58:20: 14000000 INFO @ Sun, 21 Jun 2020 21:58:22: 19000000 INFO @ Sun, 21 Jun 2020 21:58:25: 27000000 INFO @ Sun, 21 Jun 2020 21:58:27: 15000000 INFO @ Sun, 21 Jun 2020 21:58:29: 20000000 INFO @ Sun, 21 Jun 2020 21:58:31: 28000000 INFO @ Sun, 21 Jun 2020 21:58:33: 16000000 INFO @ Sun, 21 Jun 2020 21:58:35: 21000000 INFO @ Sun, 21 Jun 2020 21:58:37: 29000000 INFO @ Sun, 21 Jun 2020 21:58:40: 17000000 INFO @ Sun, 21 Jun 2020 21:58:42: 22000000 INFO @ Sun, 21 Jun 2020 21:58:43: 30000000 INFO @ Sun, 21 Jun 2020 21:58:46: 18000000 INFO @ Sun, 21 Jun 2020 21:58:48: 23000000 INFO @ Sun, 21 Jun 2020 21:58:49: 31000000 INFO @ Sun, 21 Jun 2020 21:58:53: 19000000 INFO @ Sun, 21 Jun 2020 21:58:55: 24000000 INFO @ Sun, 21 Jun 2020 21:58:55: 32000000 INFO @ Sun, 21 Jun 2020 21:58:59: 20000000 INFO @ Sun, 21 Jun 2020 21:59:01: 33000000 INFO @ Sun, 21 Jun 2020 21:59:01: 25000000 INFO @ Sun, 21 Jun 2020 21:59:06: 21000000 INFO @ Sun, 21 Jun 2020 21:59:06: 34000000 INFO @ Sun, 21 Jun 2020 21:59:07: 26000000 INFO @ Sun, 21 Jun 2020 21:59:12: 35000000 INFO @ Sun, 21 Jun 2020 21:59:13: 22000000 INFO @ Sun, 21 Jun 2020 21:59:14: 27000000 INFO @ Sun, 21 Jun 2020 21:59:18: 36000000 INFO @ Sun, 21 Jun 2020 21:59:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:59:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:59:19: #1 total tags in treatment: 36120948 INFO @ Sun, 21 Jun 2020 21:59:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:59:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:59:19: 23000000 INFO @ Sun, 21 Jun 2020 21:59:20: #1 tags after filtering in treatment: 36120946 INFO @ Sun, 21 Jun 2020 21:59:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:59:20: #1 finished! INFO @ Sun, 21 Jun 2020 21:59:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:59:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:59:20: 28000000 INFO @ Sun, 21 Jun 2020 21:59:22: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 21:59:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:59:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:59:26: 24000000 INFO @ Sun, 21 Jun 2020 21:59:27: 29000000 INFO @ Sun, 21 Jun 2020 21:59:33: 25000000 INFO @ Sun, 21 Jun 2020 21:59:34: 30000000 INFO @ Sun, 21 Jun 2020 21:59:39: 26000000 INFO @ Sun, 21 Jun 2020 21:59:40: 31000000 INFO @ Sun, 21 Jun 2020 21:59:45: 27000000 INFO @ Sun, 21 Jun 2020 21:59:47: 32000000 INFO @ Sun, 21 Jun 2020 21:59:52: 28000000 INFO @ Sun, 21 Jun 2020 21:59:53: 33000000 INFO @ Sun, 21 Jun 2020 21:59:58: 29000000 INFO @ Sun, 21 Jun 2020 21:59:59: 34000000 INFO @ Sun, 21 Jun 2020 22:00:05: 30000000 INFO @ Sun, 21 Jun 2020 22:00:06: 35000000 INFO @ Sun, 21 Jun 2020 22:00:11: 31000000 INFO @ Sun, 21 Jun 2020 22:00:12: 36000000 INFO @ Sun, 21 Jun 2020 22:00:13: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:00:13: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:00:13: #1 total tags in treatment: 36120948 INFO @ Sun, 21 Jun 2020 22:00:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:00:14: #1 tags after filtering in treatment: 36120946 INFO @ Sun, 21 Jun 2020 22:00:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:00:14: #1 finished! INFO @ Sun, 21 Jun 2020 22:00:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:00:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:00:16: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:00:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:00:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:00:18: 32000000 INFO @ Sun, 21 Jun 2020 22:00:24: 33000000 INFO @ Sun, 21 Jun 2020 22:00:30: 34000000 INFO @ Sun, 21 Jun 2020 22:00:36: 35000000 INFO @ Sun, 21 Jun 2020 22:00:43: 36000000 INFO @ Sun, 21 Jun 2020 22:00:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:00:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:00:44: #1 total tags in treatment: 36120948 INFO @ Sun, 21 Jun 2020 22:00:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:00:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:00:45: #1 tags after filtering in treatment: 36120946 INFO @ Sun, 21 Jun 2020 22:00:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:00:45: #1 finished! INFO @ Sun, 21 Jun 2020 22:00:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:00:45: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 22:00:47: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:00:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:00:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495314/SRX495314.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling