Job ID = 6458201 SRX = SRX495310 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:27:19 prefetch.2.10.7: 1) Downloading 'SRR1198815'... 2020-06-21T12:27:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:35:05 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:35:05 prefetch.2.10.7: 1) 'SRR1198815' was downloaded successfully Read 55261147 spots for SRR1198815/SRR1198815.sra Written 55261147 spots for SRR1198815/SRR1198815.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:56 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1196401 (2.16%) aligned 0 times 45510185 (82.35%) aligned exactly 1 time 8554561 (15.48%) aligned >1 times 97.84% overall alignment rate Time searching: 00:14:56 Overall time: 00:14:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17943783 / 54064746 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:04:28: 1000000 INFO @ Sun, 21 Jun 2020 22:04:33: 2000000 INFO @ Sun, 21 Jun 2020 22:04:39: 3000000 INFO @ Sun, 21 Jun 2020 22:04:44: 4000000 INFO @ Sun, 21 Jun 2020 22:04:49: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:04:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:04:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:04:55: 6000000 INFO @ Sun, 21 Jun 2020 22:04:58: 1000000 INFO @ Sun, 21 Jun 2020 22:05:00: 7000000 INFO @ Sun, 21 Jun 2020 22:05:04: 2000000 INFO @ Sun, 21 Jun 2020 22:05:06: 8000000 INFO @ Sun, 21 Jun 2020 22:05:09: 3000000 INFO @ Sun, 21 Jun 2020 22:05:12: 9000000 INFO @ Sun, 21 Jun 2020 22:05:15: 4000000 INFO @ Sun, 21 Jun 2020 22:05:17: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:05:21: 5000000 INFO @ Sun, 21 Jun 2020 22:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:05:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:05:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:05:23: 11000000 INFO @ Sun, 21 Jun 2020 22:05:26: 6000000 INFO @ Sun, 21 Jun 2020 22:05:28: 1000000 INFO @ Sun, 21 Jun 2020 22:05:28: 12000000 INFO @ Sun, 21 Jun 2020 22:05:32: 7000000 INFO @ Sun, 21 Jun 2020 22:05:34: 2000000 INFO @ Sun, 21 Jun 2020 22:05:34: 13000000 INFO @ Sun, 21 Jun 2020 22:05:38: 8000000 INFO @ Sun, 21 Jun 2020 22:05:39: 14000000 INFO @ Sun, 21 Jun 2020 22:05:40: 3000000 INFO @ Sun, 21 Jun 2020 22:05:44: 9000000 INFO @ Sun, 21 Jun 2020 22:05:45: 15000000 INFO @ Sun, 21 Jun 2020 22:05:45: 4000000 INFO @ Sun, 21 Jun 2020 22:05:49: 10000000 INFO @ Sun, 21 Jun 2020 22:05:51: 16000000 INFO @ Sun, 21 Jun 2020 22:05:51: 5000000 INFO @ Sun, 21 Jun 2020 22:05:55: 11000000 INFO @ Sun, 21 Jun 2020 22:05:56: 17000000 INFO @ Sun, 21 Jun 2020 22:05:57: 6000000 INFO @ Sun, 21 Jun 2020 22:06:01: 12000000 INFO @ Sun, 21 Jun 2020 22:06:02: 18000000 INFO @ Sun, 21 Jun 2020 22:06:03: 7000000 INFO @ Sun, 21 Jun 2020 22:06:06: 13000000 INFO @ Sun, 21 Jun 2020 22:06:07: 19000000 INFO @ Sun, 21 Jun 2020 22:06:08: 8000000 INFO @ Sun, 21 Jun 2020 22:06:12: 14000000 INFO @ Sun, 21 Jun 2020 22:06:13: 20000000 INFO @ Sun, 21 Jun 2020 22:06:14: 9000000 INFO @ Sun, 21 Jun 2020 22:06:17: 15000000 INFO @ Sun, 21 Jun 2020 22:06:18: 21000000 INFO @ Sun, 21 Jun 2020 22:06:20: 10000000 INFO @ Sun, 21 Jun 2020 22:06:23: 16000000 INFO @ Sun, 21 Jun 2020 22:06:24: 22000000 INFO @ Sun, 21 Jun 2020 22:06:26: 11000000 INFO @ Sun, 21 Jun 2020 22:06:29: 17000000 INFO @ Sun, 21 Jun 2020 22:06:29: 23000000 INFO @ Sun, 21 Jun 2020 22:06:31: 12000000 INFO @ Sun, 21 Jun 2020 22:06:34: 18000000 INFO @ Sun, 21 Jun 2020 22:06:35: 24000000 INFO @ Sun, 21 Jun 2020 22:06:37: 13000000 INFO @ Sun, 21 Jun 2020 22:06:40: 19000000 INFO @ Sun, 21 Jun 2020 22:06:40: 25000000 INFO @ Sun, 21 Jun 2020 22:06:42: 14000000 INFO @ Sun, 21 Jun 2020 22:06:45: 20000000 INFO @ Sun, 21 Jun 2020 22:06:46: 26000000 INFO @ Sun, 21 Jun 2020 22:06:48: 15000000 INFO @ Sun, 21 Jun 2020 22:06:51: 21000000 INFO @ Sun, 21 Jun 2020 22:06:52: 27000000 INFO @ Sun, 21 Jun 2020 22:06:53: 16000000 INFO @ Sun, 21 Jun 2020 22:06:56: 22000000 INFO @ Sun, 21 Jun 2020 22:06:57: 28000000 INFO @ Sun, 21 Jun 2020 22:06:59: 17000000 INFO @ Sun, 21 Jun 2020 22:07:01: 23000000 INFO @ Sun, 21 Jun 2020 22:07:03: 29000000 INFO @ Sun, 21 Jun 2020 22:07:05: 18000000 INFO @ Sun, 21 Jun 2020 22:07:07: 24000000 INFO @ Sun, 21 Jun 2020 22:07:09: 30000000 INFO @ Sun, 21 Jun 2020 22:07:10: 19000000 INFO @ Sun, 21 Jun 2020 22:07:13: 25000000 INFO @ Sun, 21 Jun 2020 22:07:15: 31000000 INFO @ Sun, 21 Jun 2020 22:07:16: 20000000 INFO @ Sun, 21 Jun 2020 22:07:18: 26000000 INFO @ Sun, 21 Jun 2020 22:07:21: 32000000 INFO @ Sun, 21 Jun 2020 22:07:22: 21000000 INFO @ Sun, 21 Jun 2020 22:07:24: 27000000 INFO @ Sun, 21 Jun 2020 22:07:26: 33000000 INFO @ Sun, 21 Jun 2020 22:07:27: 22000000 INFO @ Sun, 21 Jun 2020 22:07:29: 28000000 INFO @ Sun, 21 Jun 2020 22:07:32: 34000000 INFO @ Sun, 21 Jun 2020 22:07:32: 23000000 INFO @ Sun, 21 Jun 2020 22:07:35: 29000000 INFO @ Sun, 21 Jun 2020 22:07:38: 35000000 INFO @ Sun, 21 Jun 2020 22:07:38: 24000000 INFO @ Sun, 21 Jun 2020 22:07:40: 30000000 INFO @ Sun, 21 Jun 2020 22:07:44: 25000000 INFO @ Sun, 21 Jun 2020 22:07:44: 36000000 INFO @ Sun, 21 Jun 2020 22:07:45: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:07:45: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:07:45: #1 total tags in treatment: 36120963 INFO @ Sun, 21 Jun 2020 22:07:45: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:07:46: #1 tags after filtering in treatment: 36120962 INFO @ Sun, 21 Jun 2020 22:07:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:07:46: #1 finished! INFO @ Sun, 21 Jun 2020 22:07:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:07:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:07:46: 31000000 INFO @ Sun, 21 Jun 2020 22:07:48: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:07:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:07:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:07:49: 26000000 INFO @ Sun, 21 Jun 2020 22:07:52: 32000000 INFO @ Sun, 21 Jun 2020 22:07:54: 27000000 INFO @ Sun, 21 Jun 2020 22:07:57: 33000000 INFO @ Sun, 21 Jun 2020 22:08:00: 28000000 INFO @ Sun, 21 Jun 2020 22:08:03: 34000000 INFO @ Sun, 21 Jun 2020 22:08:05: 29000000 INFO @ Sun, 21 Jun 2020 22:08:08: 35000000 INFO @ Sun, 21 Jun 2020 22:08:11: 30000000 INFO @ Sun, 21 Jun 2020 22:08:14: 36000000 INFO @ Sun, 21 Jun 2020 22:08:15: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:08:15: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:08:15: #1 total tags in treatment: 36120963 INFO @ Sun, 21 Jun 2020 22:08:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:08:16: #1 tags after filtering in treatment: 36120962 INFO @ Sun, 21 Jun 2020 22:08:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:08:16: #1 finished! INFO @ Sun, 21 Jun 2020 22:08:16: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:08:16: 31000000 INFO @ Sun, 21 Jun 2020 22:08:18: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:08:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:08:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:08:22: 32000000 INFO @ Sun, 21 Jun 2020 22:08:27: 33000000 INFO @ Sun, 21 Jun 2020 22:08:33: 34000000 INFO @ Sun, 21 Jun 2020 22:08:38: 35000000 INFO @ Sun, 21 Jun 2020 22:08:43: 36000000 INFO @ Sun, 21 Jun 2020 22:08:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:08:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:08:44: #1 total tags in treatment: 36120963 INFO @ Sun, 21 Jun 2020 22:08:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:08:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:08:45: #1 tags after filtering in treatment: 36120962 INFO @ Sun, 21 Jun 2020 22:08:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:08:45: #1 finished! INFO @ Sun, 21 Jun 2020 22:08:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:08:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:08:48: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:08:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:08:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495310/SRX495310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。