Job ID = 6458200 SRX = SRX495309 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:13:19 prefetch.2.10.7: 1) Downloading 'SRR1198814'... 2020-06-21T12:13:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:19:29 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:19:29 prefetch.2.10.7: 1) 'SRR1198814' was downloaded successfully Read 50358398 spots for SRR1198814/SRR1198814.sra Written 50358398 spots for SRR1198814/SRR1198814.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:54 50358398 reads; of these: 50358398 (100.00%) were unpaired; of these: 1099653 (2.18%) aligned 0 times 41930476 (83.26%) aligned exactly 1 time 7328269 (14.55%) aligned >1 times 97.82% overall alignment rate Time searching: 00:11:54 Overall time: 00:11:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 15707087 / 49258745 = 0.3189 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:43:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:43:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:43:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:43:39: 1000000 INFO @ Sun, 21 Jun 2020 21:43:44: 2000000 INFO @ Sun, 21 Jun 2020 21:43:49: 3000000 INFO @ Sun, 21 Jun 2020 21:43:54: 4000000 INFO @ Sun, 21 Jun 2020 21:43:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:44:04: 6000000 INFO @ Sun, 21 Jun 2020 21:44:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:44:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:44:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:44:09: 7000000 INFO @ Sun, 21 Jun 2020 21:44:09: 1000000 INFO @ Sun, 21 Jun 2020 21:44:14: 8000000 INFO @ Sun, 21 Jun 2020 21:44:15: 2000000 INFO @ Sun, 21 Jun 2020 21:44:19: 9000000 INFO @ Sun, 21 Jun 2020 21:44:20: 3000000 INFO @ Sun, 21 Jun 2020 21:44:25: 10000000 INFO @ Sun, 21 Jun 2020 21:44:25: 4000000 INFO @ Sun, 21 Jun 2020 21:44:30: 11000000 INFO @ Sun, 21 Jun 2020 21:44:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:44:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:44:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:44:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:44:35: 12000000 INFO @ Sun, 21 Jun 2020 21:44:35: 6000000 INFO @ Sun, 21 Jun 2020 21:44:40: 1000000 INFO @ Sun, 21 Jun 2020 21:44:40: 13000000 INFO @ Sun, 21 Jun 2020 21:44:41: 7000000 INFO @ Sun, 21 Jun 2020 21:44:45: 2000000 INFO @ Sun, 21 Jun 2020 21:44:46: 14000000 INFO @ Sun, 21 Jun 2020 21:44:46: 8000000 INFO @ Sun, 21 Jun 2020 21:44:50: 3000000 INFO @ Sun, 21 Jun 2020 21:44:51: 15000000 INFO @ Sun, 21 Jun 2020 21:44:52: 9000000 INFO @ Sun, 21 Jun 2020 21:44:56: 4000000 INFO @ Sun, 21 Jun 2020 21:44:56: 16000000 INFO @ Sun, 21 Jun 2020 21:44:57: 10000000 INFO @ Sun, 21 Jun 2020 21:45:01: 5000000 INFO @ Sun, 21 Jun 2020 21:45:02: 17000000 INFO @ Sun, 21 Jun 2020 21:45:02: 11000000 INFO @ Sun, 21 Jun 2020 21:45:06: 6000000 INFO @ Sun, 21 Jun 2020 21:45:07: 18000000 INFO @ Sun, 21 Jun 2020 21:45:08: 12000000 INFO @ Sun, 21 Jun 2020 21:45:12: 7000000 INFO @ Sun, 21 Jun 2020 21:45:12: 19000000 INFO @ Sun, 21 Jun 2020 21:45:13: 13000000 INFO @ Sun, 21 Jun 2020 21:45:17: 8000000 INFO @ Sun, 21 Jun 2020 21:45:18: 20000000 INFO @ Sun, 21 Jun 2020 21:45:18: 14000000 INFO @ Sun, 21 Jun 2020 21:45:22: 9000000 INFO @ Sun, 21 Jun 2020 21:45:23: 21000000 INFO @ Sun, 21 Jun 2020 21:45:24: 15000000 INFO @ Sun, 21 Jun 2020 21:45:28: 10000000 INFO @ Sun, 21 Jun 2020 21:45:28: 22000000 INFO @ Sun, 21 Jun 2020 21:45:29: 16000000 INFO @ Sun, 21 Jun 2020 21:45:33: 11000000 INFO @ Sun, 21 Jun 2020 21:45:34: 23000000 INFO @ Sun, 21 Jun 2020 21:45:35: 17000000 INFO @ Sun, 21 Jun 2020 21:45:39: 12000000 INFO @ Sun, 21 Jun 2020 21:45:39: 24000000 INFO @ Sun, 21 Jun 2020 21:45:40: 18000000 INFO @ Sun, 21 Jun 2020 21:45:44: 13000000 INFO @ Sun, 21 Jun 2020 21:45:45: 25000000 INFO @ Sun, 21 Jun 2020 21:45:46: 19000000 INFO @ Sun, 21 Jun 2020 21:45:49: 14000000 INFO @ Sun, 21 Jun 2020 21:45:50: 26000000 INFO @ Sun, 21 Jun 2020 21:45:51: 20000000 INFO @ Sun, 21 Jun 2020 21:45:55: 15000000 INFO @ Sun, 21 Jun 2020 21:45:56: 27000000 INFO @ Sun, 21 Jun 2020 21:45:57: 21000000 INFO @ Sun, 21 Jun 2020 21:46:00: 16000000 INFO @ Sun, 21 Jun 2020 21:46:02: 28000000 INFO @ Sun, 21 Jun 2020 21:46:02: 22000000 INFO @ Sun, 21 Jun 2020 21:46:06: 17000000 INFO @ Sun, 21 Jun 2020 21:46:07: 29000000 INFO @ Sun, 21 Jun 2020 21:46:07: 23000000 INFO @ Sun, 21 Jun 2020 21:46:11: 18000000 INFO @ Sun, 21 Jun 2020 21:46:12: 30000000 INFO @ Sun, 21 Jun 2020 21:46:13: 24000000 INFO @ Sun, 21 Jun 2020 21:46:16: 19000000 INFO @ Sun, 21 Jun 2020 21:46:18: 31000000 INFO @ Sun, 21 Jun 2020 21:46:18: 25000000 INFO @ Sun, 21 Jun 2020 21:46:22: 20000000 INFO @ Sun, 21 Jun 2020 21:46:23: 32000000 INFO @ Sun, 21 Jun 2020 21:46:24: 26000000 INFO @ Sun, 21 Jun 2020 21:46:27: 21000000 INFO @ Sun, 21 Jun 2020 21:46:29: 33000000 INFO @ Sun, 21 Jun 2020 21:46:29: 27000000 INFO @ Sun, 21 Jun 2020 21:46:32: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:46:32: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:46:32: #1 total tags in treatment: 33551658 INFO @ Sun, 21 Jun 2020 21:46:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:46:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:46:33: 22000000 INFO @ Sun, 21 Jun 2020 21:46:33: #1 tags after filtering in treatment: 33551655 INFO @ Sun, 21 Jun 2020 21:46:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:46:33: #1 finished! INFO @ Sun, 21 Jun 2020 21:46:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:46:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:46:35: 28000000 INFO @ Sun, 21 Jun 2020 21:46:35: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:46:35: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:46:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:46:38: 23000000 INFO @ Sun, 21 Jun 2020 21:46:41: 29000000 INFO @ Sun, 21 Jun 2020 21:46:43: 24000000 INFO @ Sun, 21 Jun 2020 21:46:46: 30000000 INFO @ Sun, 21 Jun 2020 21:46:49: 25000000 INFO @ Sun, 21 Jun 2020 21:46:51: 31000000 INFO @ Sun, 21 Jun 2020 21:46:54: 26000000 INFO @ Sun, 21 Jun 2020 21:46:57: 32000000 INFO @ Sun, 21 Jun 2020 21:47:00: 27000000 INFO @ Sun, 21 Jun 2020 21:47:02: 33000000 INFO @ Sun, 21 Jun 2020 21:47:06: 28000000 INFO @ Sun, 21 Jun 2020 21:47:06: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:47:06: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:47:06: #1 total tags in treatment: 33551658 INFO @ Sun, 21 Jun 2020 21:47:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:47:07: #1 tags after filtering in treatment: 33551655 INFO @ Sun, 21 Jun 2020 21:47:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:47:07: #1 finished! INFO @ Sun, 21 Jun 2020 21:47:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:47:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:47:09: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:47:09: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:47:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:47:11: 29000000 INFO @ Sun, 21 Jun 2020 21:47:16: 30000000 INFO @ Sun, 21 Jun 2020 21:47:21: 31000000 INFO @ Sun, 21 Jun 2020 21:47:26: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:47:32: 33000000 INFO @ Sun, 21 Jun 2020 21:47:35: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:47:35: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:47:35: #1 total tags in treatment: 33551658 INFO @ Sun, 21 Jun 2020 21:47:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:47:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:47:36: #1 tags after filtering in treatment: 33551655 INFO @ Sun, 21 Jun 2020 21:47:36: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:47:36: #1 finished! INFO @ Sun, 21 Jun 2020 21:47:36: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:47:38: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:47:38: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:47:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495309/SRX495309.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。