Job ID = 6458199 SRX = SRX495308 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:21:04 prefetch.2.10.7: 1) Downloading 'SRR1198813'... 2020-06-21T12:21:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:29:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:29:56 prefetch.2.10.7: 1) 'SRR1198813' was downloaded successfully Read 55261147 spots for SRR1198813/SRR1198813.sra Written 55261147 spots for SRR1198813/SRR1198813.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:38 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1196442 (2.17%) aligned 0 times 45510172 (82.35%) aligned exactly 1 time 8554533 (15.48%) aligned >1 times 97.83% overall alignment rate Time searching: 00:14:38 Overall time: 00:14:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17943400 / 54064705 = 0.3319 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:59:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:59:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:59:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:59:46: 1000000 INFO @ Sun, 21 Jun 2020 21:59:52: 2000000 INFO @ Sun, 21 Jun 2020 21:59:57: 3000000 INFO @ Sun, 21 Jun 2020 22:00:03: 4000000 INFO @ Sun, 21 Jun 2020 22:00:08: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:00:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:00:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:00:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:00:14: 6000000 INFO @ Sun, 21 Jun 2020 22:00:18: 1000000 INFO @ Sun, 21 Jun 2020 22:00:20: 7000000 INFO @ Sun, 21 Jun 2020 22:00:24: 2000000 INFO @ Sun, 21 Jun 2020 22:00:26: 8000000 INFO @ Sun, 21 Jun 2020 22:00:31: 3000000 INFO @ Sun, 21 Jun 2020 22:00:32: 9000000 INFO @ Sun, 21 Jun 2020 22:00:37: 4000000 INFO @ Sun, 21 Jun 2020 22:00:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 22:00:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 22:00:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 22:00:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 22:00:44: 5000000 INFO @ Sun, 21 Jun 2020 22:00:44: 11000000 INFO @ Sun, 21 Jun 2020 22:00:47: 1000000 INFO @ Sun, 21 Jun 2020 22:00:50: 12000000 INFO @ Sun, 21 Jun 2020 22:00:51: 6000000 INFO @ Sun, 21 Jun 2020 22:00:54: 2000000 INFO @ Sun, 21 Jun 2020 22:00:57: 13000000 INFO @ Sun, 21 Jun 2020 22:00:57: 7000000 INFO @ Sun, 21 Jun 2020 22:01:00: 3000000 INFO @ Sun, 21 Jun 2020 22:01:03: 14000000 INFO @ Sun, 21 Jun 2020 22:01:04: 8000000 INFO @ Sun, 21 Jun 2020 22:01:06: 4000000 INFO @ Sun, 21 Jun 2020 22:01:09: 15000000 INFO @ Sun, 21 Jun 2020 22:01:11: 9000000 INFO @ Sun, 21 Jun 2020 22:01:12: 5000000 INFO @ Sun, 21 Jun 2020 22:01:16: 16000000 INFO @ Sun, 21 Jun 2020 22:01:17: 10000000 INFO @ Sun, 21 Jun 2020 22:01:19: 6000000 INFO @ Sun, 21 Jun 2020 22:01:22: 17000000 INFO @ Sun, 21 Jun 2020 22:01:24: 11000000 INFO @ Sun, 21 Jun 2020 22:01:25: 7000000 INFO @ Sun, 21 Jun 2020 22:01:28: 18000000 INFO @ Sun, 21 Jun 2020 22:01:30: 12000000 INFO @ Sun, 21 Jun 2020 22:01:31: 8000000 INFO @ Sun, 21 Jun 2020 22:01:35: 19000000 INFO @ Sun, 21 Jun 2020 22:01:37: 13000000 INFO @ Sun, 21 Jun 2020 22:01:38: 9000000 INFO @ Sun, 21 Jun 2020 22:01:41: 20000000 INFO @ Sun, 21 Jun 2020 22:01:44: 14000000 INFO @ Sun, 21 Jun 2020 22:01:44: 10000000 INFO @ Sun, 21 Jun 2020 22:01:48: 21000000 INFO @ Sun, 21 Jun 2020 22:01:50: 15000000 INFO @ Sun, 21 Jun 2020 22:01:51: 11000000 INFO @ Sun, 21 Jun 2020 22:01:54: 22000000 INFO @ Sun, 21 Jun 2020 22:01:57: 16000000 INFO @ Sun, 21 Jun 2020 22:01:57: 12000000 INFO @ Sun, 21 Jun 2020 22:02:00: 23000000 INFO @ Sun, 21 Jun 2020 22:02:03: 17000000 INFO @ Sun, 21 Jun 2020 22:02:04: 13000000 INFO @ Sun, 21 Jun 2020 22:02:06: 24000000 INFO @ Sun, 21 Jun 2020 22:02:10: 14000000 INFO @ Sun, 21 Jun 2020 22:02:10: 18000000 INFO @ Sun, 21 Jun 2020 22:02:13: 25000000 INFO @ Sun, 21 Jun 2020 22:02:17: 15000000 INFO @ Sun, 21 Jun 2020 22:02:17: 19000000 INFO @ Sun, 21 Jun 2020 22:02:19: 26000000 INFO @ Sun, 21 Jun 2020 22:02:23: 16000000 INFO @ Sun, 21 Jun 2020 22:02:23: 20000000 INFO @ Sun, 21 Jun 2020 22:02:26: 27000000 INFO @ Sun, 21 Jun 2020 22:02:29: 17000000 INFO @ Sun, 21 Jun 2020 22:02:30: 21000000 INFO @ Sun, 21 Jun 2020 22:02:32: 28000000 INFO @ Sun, 21 Jun 2020 22:02:36: 18000000 INFO @ Sun, 21 Jun 2020 22:02:36: 22000000 INFO @ Sun, 21 Jun 2020 22:02:38: 29000000 INFO @ Sun, 21 Jun 2020 22:02:42: 19000000 INFO @ Sun, 21 Jun 2020 22:02:43: 23000000 INFO @ Sun, 21 Jun 2020 22:02:45: 30000000 INFO @ Sun, 21 Jun 2020 22:02:49: 20000000 INFO @ Sun, 21 Jun 2020 22:02:49: 24000000 INFO @ Sun, 21 Jun 2020 22:02:51: 31000000 INFO @ Sun, 21 Jun 2020 22:02:55: 21000000 INFO @ Sun, 21 Jun 2020 22:02:55: 25000000 INFO @ Sun, 21 Jun 2020 22:02:57: 32000000 INFO @ Sun, 21 Jun 2020 22:03:01: 22000000 INFO @ Sun, 21 Jun 2020 22:03:02: 26000000 INFO @ Sun, 21 Jun 2020 22:03:03: 33000000 INFO @ Sun, 21 Jun 2020 22:03:07: 23000000 INFO @ Sun, 21 Jun 2020 22:03:08: 27000000 INFO @ Sun, 21 Jun 2020 22:03:09: 34000000 INFO @ Sun, 21 Jun 2020 22:03:13: 24000000 INFO @ Sun, 21 Jun 2020 22:03:15: 28000000 INFO @ Sun, 21 Jun 2020 22:03:15: 35000000 INFO @ Sun, 21 Jun 2020 22:03:20: 25000000 INFO @ Sun, 21 Jun 2020 22:03:21: 29000000 INFO @ Sun, 21 Jun 2020 22:03:22: 36000000 INFO @ Sun, 21 Jun 2020 22:03:23: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:03:23: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:03:23: #1 total tags in treatment: 36121305 INFO @ Sun, 21 Jun 2020 22:03:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:03:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:03:24: #1 tags after filtering in treatment: 36121303 INFO @ Sun, 21 Jun 2020 22:03:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:03:24: #1 finished! INFO @ Sun, 21 Jun 2020 22:03:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:03:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:03:26: 26000000 INFO @ Sun, 21 Jun 2020 22:03:26: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:03:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:03:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:03:28: 30000000 INFO @ Sun, 21 Jun 2020 22:03:32: 27000000 INFO @ Sun, 21 Jun 2020 22:03:34: 31000000 INFO @ Sun, 21 Jun 2020 22:03:38: 28000000 INFO @ Sun, 21 Jun 2020 22:03:41: 32000000 INFO @ Sun, 21 Jun 2020 22:03:44: 29000000 INFO @ Sun, 21 Jun 2020 22:03:47: 33000000 INFO @ Sun, 21 Jun 2020 22:03:51: 30000000 INFO @ Sun, 21 Jun 2020 22:03:53: 34000000 INFO @ Sun, 21 Jun 2020 22:03:57: 31000000 INFO @ Sun, 21 Jun 2020 22:04:00: 35000000 INFO @ Sun, 21 Jun 2020 22:04:03: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 22:04:06: 36000000 INFO @ Sun, 21 Jun 2020 22:04:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:04:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:04:07: #1 total tags in treatment: 36121305 INFO @ Sun, 21 Jun 2020 22:04:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:08: #1 tags after filtering in treatment: 36121303 INFO @ Sun, 21 Jun 2020 22:04:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:08: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:09: 33000000 INFO @ Sun, 21 Jun 2020 22:04:11: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:04:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 22:04:15: 34000000 INFO @ Sun, 21 Jun 2020 22:04:21: 35000000 INFO @ Sun, 21 Jun 2020 22:04:27: 36000000 INFO @ Sun, 21 Jun 2020 22:04:28: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 22:04:28: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 22:04:28: #1 total tags in treatment: 36121305 INFO @ Sun, 21 Jun 2020 22:04:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 22:04:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 22:04:29: #1 tags after filtering in treatment: 36121303 INFO @ Sun, 21 Jun 2020 22:04:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 22:04:29: #1 finished! INFO @ Sun, 21 Jun 2020 22:04:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 22:04:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 22:04:31: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 22:04:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 22:04:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495308/SRX495308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。