Job ID = 6458194 SRX = SRX495304 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:14:04 prefetch.2.10.7: 1) Downloading 'SRR1198809'... 2020-06-21T12:14:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:19:04 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:19:04 prefetch.2.10.7: 1) 'SRR1198809' was downloaded successfully Read 35836077 spots for SRR1198809/SRR1198809.sra Written 35836077 spots for SRR1198809/SRR1198809.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:11 35836077 reads; of these: 35836077 (100.00%) were unpaired; of these: 3744228 (10.45%) aligned 0 times 27832427 (77.67%) aligned exactly 1 time 4259422 (11.89%) aligned >1 times 89.55% overall alignment rate Time searching: 00:08:11 Overall time: 00:08:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8717798 / 32091849 = 0.2717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:36:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:36:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:36:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:36:39: 1000000 INFO @ Sun, 21 Jun 2020 21:36:44: 2000000 INFO @ Sun, 21 Jun 2020 21:36:50: 3000000 INFO @ Sun, 21 Jun 2020 21:36:55: 4000000 INFO @ Sun, 21 Jun 2020 21:37:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:37:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:37:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:37:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:37:06: 6000000 INFO @ Sun, 21 Jun 2020 21:37:10: 1000000 INFO @ Sun, 21 Jun 2020 21:37:13: 7000000 INFO @ Sun, 21 Jun 2020 21:37:16: 2000000 INFO @ Sun, 21 Jun 2020 21:37:19: 8000000 INFO @ Sun, 21 Jun 2020 21:37:22: 3000000 INFO @ Sun, 21 Jun 2020 21:37:25: 9000000 INFO @ Sun, 21 Jun 2020 21:37:28: 4000000 INFO @ Sun, 21 Jun 2020 21:37:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:37:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:37:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:37:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:37:34: 5000000 INFO @ Sun, 21 Jun 2020 21:37:37: 11000000 INFO @ Sun, 21 Jun 2020 21:37:40: 1000000 INFO @ Sun, 21 Jun 2020 21:37:41: 6000000 INFO @ Sun, 21 Jun 2020 21:37:44: 12000000 INFO @ Sun, 21 Jun 2020 21:37:46: 2000000 INFO @ Sun, 21 Jun 2020 21:37:47: 7000000 INFO @ Sun, 21 Jun 2020 21:37:50: 13000000 INFO @ Sun, 21 Jun 2020 21:37:53: 3000000 INFO @ Sun, 21 Jun 2020 21:37:53: 8000000 INFO @ Sun, 21 Jun 2020 21:37:56: 14000000 INFO @ Sun, 21 Jun 2020 21:37:59: 4000000 INFO @ Sun, 21 Jun 2020 21:38:00: 9000000 INFO @ Sun, 21 Jun 2020 21:38:02: 15000000 INFO @ Sun, 21 Jun 2020 21:38:05: 5000000 INFO @ Sun, 21 Jun 2020 21:38:06: 10000000 INFO @ Sun, 21 Jun 2020 21:38:09: 16000000 INFO @ Sun, 21 Jun 2020 21:38:11: 6000000 INFO @ Sun, 21 Jun 2020 21:38:12: 11000000 INFO @ Sun, 21 Jun 2020 21:38:15: 17000000 INFO @ Sun, 21 Jun 2020 21:38:17: 7000000 INFO @ Sun, 21 Jun 2020 21:38:18: 12000000 INFO @ Sun, 21 Jun 2020 21:38:21: 18000000 INFO @ Sun, 21 Jun 2020 21:38:24: 8000000 INFO @ Sun, 21 Jun 2020 21:38:24: 13000000 INFO @ Sun, 21 Jun 2020 21:38:27: 19000000 INFO @ Sun, 21 Jun 2020 21:38:30: 9000000 INFO @ Sun, 21 Jun 2020 21:38:30: 14000000 INFO @ Sun, 21 Jun 2020 21:38:34: 20000000 INFO @ Sun, 21 Jun 2020 21:38:36: 10000000 INFO @ Sun, 21 Jun 2020 21:38:36: 15000000 INFO @ Sun, 21 Jun 2020 21:38:40: 21000000 INFO @ Sun, 21 Jun 2020 21:38:42: 11000000 INFO @ Sun, 21 Jun 2020 21:38:42: 16000000 INFO @ Sun, 21 Jun 2020 21:38:46: 22000000 INFO @ Sun, 21 Jun 2020 21:38:48: 12000000 INFO @ Sun, 21 Jun 2020 21:38:49: 17000000 INFO @ Sun, 21 Jun 2020 21:38:52: 23000000 INFO @ Sun, 21 Jun 2020 21:38:54: 13000000 INFO @ Sun, 21 Jun 2020 21:38:54: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:38:54: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:38:54: #1 total tags in treatment: 23374051 INFO @ Sun, 21 Jun 2020 21:38:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:38:55: 18000000 INFO @ Sun, 21 Jun 2020 21:38:55: #1 tags after filtering in treatment: 23374040 INFO @ Sun, 21 Jun 2020 21:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:38:55: #1 finished! INFO @ Sun, 21 Jun 2020 21:38:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:38:56: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:38:56: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:38:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:39:00: 14000000 INFO @ Sun, 21 Jun 2020 21:39:01: 19000000 INFO @ Sun, 21 Jun 2020 21:39:06: 15000000 INFO @ Sun, 21 Jun 2020 21:39:07: 20000000 INFO @ Sun, 21 Jun 2020 21:39:12: 16000000 INFO @ Sun, 21 Jun 2020 21:39:13: 21000000 INFO @ Sun, 21 Jun 2020 21:39:18: 17000000 INFO @ Sun, 21 Jun 2020 21:39:19: 22000000 INFO @ Sun, 21 Jun 2020 21:39:24: 18000000 INFO @ Sun, 21 Jun 2020 21:39:25: 23000000 INFO @ Sun, 21 Jun 2020 21:39:27: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:39:27: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:39:27: #1 total tags in treatment: 23374051 INFO @ Sun, 21 Jun 2020 21:39:27: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:39:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:39:28: #1 tags after filtering in treatment: 23374040 INFO @ Sun, 21 Jun 2020 21:39:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:39:28: #1 finished! INFO @ Sun, 21 Jun 2020 21:39:28: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:39:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:39:29: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:39:29: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:39:29: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 21:39:30: 19000000 cut: /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:39:35: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:39:41: 21000000 INFO @ Sun, 21 Jun 2020 21:39:46: 22000000 INFO @ Sun, 21 Jun 2020 21:39:52: 23000000 INFO @ Sun, 21 Jun 2020 21:39:54: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:39:54: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:39:54: #1 total tags in treatment: 23374051 INFO @ Sun, 21 Jun 2020 21:39:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:39:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:39:55: #1 tags after filtering in treatment: 23374040 INFO @ Sun, 21 Jun 2020 21:39:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:39:55: #1 finished! INFO @ Sun, 21 Jun 2020 21:39:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:39:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:39:56: #2 number of paired peaks: 6 WARNING @ Sun, 21 Jun 2020 21:39:56: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:39:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495304/SRX495304.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。