Job ID = 6458193 SRX = SRX495303 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:24:04 prefetch.2.10.7: 1) Downloading 'SRR1198808'... 2020-06-21T12:24:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:30:52 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:30:52 prefetch.2.10.7: 1) 'SRR1198808' was downloaded successfully Read 32960289 spots for SRR1198808/SRR1198808.sra Written 32960289 spots for SRR1198808/SRR1198808.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:44 32960289 reads; of these: 32960289 (100.00%) were unpaired; of these: 5005224 (15.19%) aligned 0 times 22718063 (68.93%) aligned exactly 1 time 5237002 (15.89%) aligned >1 times 84.81% overall alignment rate Time searching: 00:07:44 Overall time: 00:07:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6831560 / 27955065 = 0.2444 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:14: 1000000 INFO @ Sun, 21 Jun 2020 21:47:19: 2000000 INFO @ Sun, 21 Jun 2020 21:47:25: 3000000 INFO @ Sun, 21 Jun 2020 21:47:30: 4000000 INFO @ Sun, 21 Jun 2020 21:47:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:47:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:47:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:47:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:47:41: 6000000 INFO @ Sun, 21 Jun 2020 21:47:44: 1000000 INFO @ Sun, 21 Jun 2020 21:47:47: 7000000 INFO @ Sun, 21 Jun 2020 21:47:50: 2000000 INFO @ Sun, 21 Jun 2020 21:47:52: 8000000 INFO @ Sun, 21 Jun 2020 21:47:56: 3000000 INFO @ Sun, 21 Jun 2020 21:47:58: 9000000 INFO @ Sun, 21 Jun 2020 21:48:02: 4000000 INFO @ Sun, 21 Jun 2020 21:48:04: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:48:08: 5000000 INFO @ Sun, 21 Jun 2020 21:48:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:48:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:48:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:48:10: 11000000 INFO @ Sun, 21 Jun 2020 21:48:14: 6000000 INFO @ Sun, 21 Jun 2020 21:48:14: 1000000 INFO @ Sun, 21 Jun 2020 21:48:16: 12000000 INFO @ Sun, 21 Jun 2020 21:48:20: 7000000 INFO @ Sun, 21 Jun 2020 21:48:20: 2000000 INFO @ Sun, 21 Jun 2020 21:48:22: 13000000 INFO @ Sun, 21 Jun 2020 21:48:26: 8000000 INFO @ Sun, 21 Jun 2020 21:48:26: 3000000 INFO @ Sun, 21 Jun 2020 21:48:28: 14000000 INFO @ Sun, 21 Jun 2020 21:48:31: 9000000 INFO @ Sun, 21 Jun 2020 21:48:32: 4000000 INFO @ Sun, 21 Jun 2020 21:48:34: 15000000 INFO @ Sun, 21 Jun 2020 21:48:37: 10000000 INFO @ Sun, 21 Jun 2020 21:48:38: 5000000 INFO @ Sun, 21 Jun 2020 21:48:39: 16000000 INFO @ Sun, 21 Jun 2020 21:48:43: 11000000 INFO @ Sun, 21 Jun 2020 21:48:43: 6000000 INFO @ Sun, 21 Jun 2020 21:48:45: 17000000 INFO @ Sun, 21 Jun 2020 21:48:49: 12000000 INFO @ Sun, 21 Jun 2020 21:48:49: 7000000 INFO @ Sun, 21 Jun 2020 21:48:51: 18000000 INFO @ Sun, 21 Jun 2020 21:48:55: 13000000 INFO @ Sun, 21 Jun 2020 21:48:55: 8000000 INFO @ Sun, 21 Jun 2020 21:48:57: 19000000 INFO @ Sun, 21 Jun 2020 21:49:01: 14000000 INFO @ Sun, 21 Jun 2020 21:49:01: 9000000 INFO @ Sun, 21 Jun 2020 21:49:03: 20000000 INFO @ Sun, 21 Jun 2020 21:49:06: 15000000 INFO @ Sun, 21 Jun 2020 21:49:07: 10000000 INFO @ Sun, 21 Jun 2020 21:49:09: 21000000 INFO @ Sun, 21 Jun 2020 21:49:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:49:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:49:09: #1 total tags in treatment: 21123505 INFO @ Sun, 21 Jun 2020 21:49:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:49:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:49:10: #1 tags after filtering in treatment: 21123502 INFO @ Sun, 21 Jun 2020 21:49:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:49:10: #1 finished! INFO @ Sun, 21 Jun 2020 21:49:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:49:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:49:11: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:49:11: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:49:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:49:12: 16000000 INFO @ Sun, 21 Jun 2020 21:49:12: 11000000 INFO @ Sun, 21 Jun 2020 21:49:18: 17000000 INFO @ Sun, 21 Jun 2020 21:49:18: 12000000 INFO @ Sun, 21 Jun 2020 21:49:23: 13000000 INFO @ Sun, 21 Jun 2020 21:49:23: 18000000 INFO @ Sun, 21 Jun 2020 21:49:29: 14000000 INFO @ Sun, 21 Jun 2020 21:49:29: 19000000 INFO @ Sun, 21 Jun 2020 21:49:34: 15000000 INFO @ Sun, 21 Jun 2020 21:49:34: 20000000 INFO @ Sun, 21 Jun 2020 21:49:39: 16000000 INFO @ Sun, 21 Jun 2020 21:49:40: 21000000 INFO @ Sun, 21 Jun 2020 21:49:41: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:49:41: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:49:41: #1 total tags in treatment: 21123505 INFO @ Sun, 21 Jun 2020 21:49:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:49:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:49:42: #1 tags after filtering in treatment: 21123502 INFO @ Sun, 21 Jun 2020 21:49:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:49:42: #1 finished! INFO @ Sun, 21 Jun 2020 21:49:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:49:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:49:43: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:49:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:49:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:49:45: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:49:50: 18000000 INFO @ Sun, 21 Jun 2020 21:49:56: 19000000 INFO @ Sun, 21 Jun 2020 21:50:01: 20000000 INFO @ Sun, 21 Jun 2020 21:50:06: 21000000 INFO @ Sun, 21 Jun 2020 21:50:07: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:50:07: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:50:07: #1 total tags in treatment: 21123505 INFO @ Sun, 21 Jun 2020 21:50:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:50:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:50:08: #1 tags after filtering in treatment: 21123502 INFO @ Sun, 21 Jun 2020 21:50:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:50:08: #1 finished! INFO @ Sun, 21 Jun 2020 21:50:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:50:09: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 21:50:09: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:50:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495303/SRX495303.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。