Job ID = 6458192 SRX = SRX495302 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T12:23:04 prefetch.2.10.7: 1) Downloading 'SRR1198807'... 2020-06-21T12:23:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T12:31:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T12:31:00 prefetch.2.10.7: 1) 'SRR1198807' was downloaded successfully Read 41603816 spots for SRR1198807/SRR1198807.sra Written 41603816 spots for SRR1198807/SRR1198807.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:11 41603816 reads; of these: 41603816 (100.00%) were unpaired; of these: 5464136 (13.13%) aligned 0 times 30564643 (73.47%) aligned exactly 1 time 5575037 (13.40%) aligned >1 times 86.87% overall alignment rate Time searching: 00:10:11 Overall time: 00:10:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9942069 / 36139680 = 0.2751 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:52:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:52:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:52:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:52:50: 1000000 INFO @ Sun, 21 Jun 2020 21:52:56: 2000000 INFO @ Sun, 21 Jun 2020 21:53:02: 3000000 INFO @ Sun, 21 Jun 2020 21:53:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:14: 5000000 INFO @ Sun, 21 Jun 2020 21:53:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:20: 6000000 INFO @ Sun, 21 Jun 2020 21:53:20: 1000000 INFO @ Sun, 21 Jun 2020 21:53:25: 2000000 INFO @ Sun, 21 Jun 2020 21:53:26: 7000000 INFO @ Sun, 21 Jun 2020 21:53:30: 3000000 INFO @ Sun, 21 Jun 2020 21:53:32: 8000000 INFO @ Sun, 21 Jun 2020 21:53:35: 4000000 INFO @ Sun, 21 Jun 2020 21:53:38: 9000000 INFO @ Sun, 21 Jun 2020 21:53:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 21:53:44: 10000000 INFO @ Sun, 21 Jun 2020 21:53:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 21:53:44: #1 read tag files... INFO @ Sun, 21 Jun 2020 21:53:44: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 21:53:46: 6000000 INFO @ Sun, 21 Jun 2020 21:53:49: 11000000 INFO @ Sun, 21 Jun 2020 21:53:50: 1000000 INFO @ Sun, 21 Jun 2020 21:53:51: 7000000 INFO @ Sun, 21 Jun 2020 21:53:55: 12000000 INFO @ Sun, 21 Jun 2020 21:53:56: 2000000 INFO @ Sun, 21 Jun 2020 21:53:57: 8000000 INFO @ Sun, 21 Jun 2020 21:54:01: 3000000 INFO @ Sun, 21 Jun 2020 21:54:01: 13000000 INFO @ Sun, 21 Jun 2020 21:54:02: 9000000 INFO @ Sun, 21 Jun 2020 21:54:07: 4000000 INFO @ Sun, 21 Jun 2020 21:54:07: 14000000 INFO @ Sun, 21 Jun 2020 21:54:08: 10000000 INFO @ Sun, 21 Jun 2020 21:54:12: 5000000 INFO @ Sun, 21 Jun 2020 21:54:13: 11000000 INFO @ Sun, 21 Jun 2020 21:54:13: 15000000 INFO @ Sun, 21 Jun 2020 21:54:17: 6000000 INFO @ Sun, 21 Jun 2020 21:54:18: 12000000 INFO @ Sun, 21 Jun 2020 21:54:19: 16000000 INFO @ Sun, 21 Jun 2020 21:54:22: 7000000 INFO @ Sun, 21 Jun 2020 21:54:24: 13000000 INFO @ Sun, 21 Jun 2020 21:54:25: 17000000 INFO @ Sun, 21 Jun 2020 21:54:28: 8000000 INFO @ Sun, 21 Jun 2020 21:54:29: 14000000 INFO @ Sun, 21 Jun 2020 21:54:31: 18000000 INFO @ Sun, 21 Jun 2020 21:54:33: 9000000 INFO @ Sun, 21 Jun 2020 21:54:34: 15000000 INFO @ Sun, 21 Jun 2020 21:54:37: 19000000 INFO @ Sun, 21 Jun 2020 21:54:38: 10000000 INFO @ Sun, 21 Jun 2020 21:54:40: 16000000 INFO @ Sun, 21 Jun 2020 21:54:43: 20000000 INFO @ Sun, 21 Jun 2020 21:54:44: 11000000 INFO @ Sun, 21 Jun 2020 21:54:45: 17000000 INFO @ Sun, 21 Jun 2020 21:54:49: 21000000 INFO @ Sun, 21 Jun 2020 21:54:49: 12000000 INFO @ Sun, 21 Jun 2020 21:54:50: 18000000 INFO @ Sun, 21 Jun 2020 21:54:54: 13000000 INFO @ Sun, 21 Jun 2020 21:54:55: 22000000 INFO @ Sun, 21 Jun 2020 21:54:56: 19000000 INFO @ Sun, 21 Jun 2020 21:55:00: 14000000 INFO @ Sun, 21 Jun 2020 21:55:01: 20000000 INFO @ Sun, 21 Jun 2020 21:55:01: 23000000 INFO @ Sun, 21 Jun 2020 21:55:05: 15000000 INFO @ Sun, 21 Jun 2020 21:55:06: 21000000 INFO @ Sun, 21 Jun 2020 21:55:07: 24000000 INFO @ Sun, 21 Jun 2020 21:55:10: 16000000 INFO @ Sun, 21 Jun 2020 21:55:12: 22000000 INFO @ Sun, 21 Jun 2020 21:55:13: 25000000 INFO @ Sun, 21 Jun 2020 21:55:15: 17000000 INFO @ Sun, 21 Jun 2020 21:55:17: 23000000 INFO @ Sun, 21 Jun 2020 21:55:19: 26000000 INFO @ Sun, 21 Jun 2020 21:55:20: 18000000 INFO @ Sun, 21 Jun 2020 21:55:21: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:21: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:21: #1 total tags in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:55:21: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:21: #1 tags after filtering in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:55:21: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:21: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:21: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:21: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:22: 24000000 INFO @ Sun, 21 Jun 2020 21:55:23: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:55:23: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:25: 19000000 INFO @ Sun, 21 Jun 2020 21:55:27: 25000000 INFO @ Sun, 21 Jun 2020 21:55:30: 20000000 INFO @ Sun, 21 Jun 2020 21:55:33: 26000000 INFO @ Sun, 21 Jun 2020 21:55:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:55:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:55:34: #1 total tags in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:55:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:55:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:55:35: #1 tags after filtering in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:55:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:55:35: #1 finished! INFO @ Sun, 21 Jun 2020 21:55:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:55:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:55:35: 21000000 INFO @ Sun, 21 Jun 2020 21:55:36: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:55:36: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:55:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 21:55:41: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 21:55:46: 23000000 INFO @ Sun, 21 Jun 2020 21:55:51: 24000000 INFO @ Sun, 21 Jun 2020 21:55:56: 25000000 INFO @ Sun, 21 Jun 2020 21:56:01: 26000000 INFO @ Sun, 21 Jun 2020 21:56:02: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 21:56:02: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 21:56:02: #1 total tags in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:56:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 21:56:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 21:56:03: #1 tags after filtering in treatment: 26197611 INFO @ Sun, 21 Jun 2020 21:56:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 21:56:03: #1 finished! INFO @ Sun, 21 Jun 2020 21:56:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 21:56:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 21:56:05: #2 number of paired peaks: 1 WARNING @ Sun, 21 Jun 2020 21:56:05: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 21:56:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX495302/SRX495302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。